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Epigenetic regulation of proliferation and differentiation of hematopoietic stem cells

Research Project

Project/Area Number 17390300
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionShinshu University

Principal Investigator

KOIKE Kenichi  Shinshu University, Department of Pediatrics, Professor (40143979)

Co-Investigator(Kenkyū-buntansha) SHIOHARA Masaaki  Shinshu University, Department of Pediatrics, Assistant Professor (00293514)
NAKAZAWA Yozo  Shinshu University, Department of Pediatrics, Senior Assistant Professor (60397312)
坂下 一夫  信州大学, 医学部, 助教 (10345746)
上條 岳彦  信州大学, 医学部附属病院, 講師 (90262708)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsStem cells / GM-CSF / p15 / methylation / histone acetylation / MO7F Cells / AML / p15遺伝子 / DNAメチル化 / ヒストン修飾 / アセチル化ヒストンH3 / メチル化ヒストンH3 / 造血幹細胞 / GM-CSF / MO7e細胞 / メチル化
Research Abstract

In the present study, to understand the process of GM-CSF-induced methylation in the CpG. island region of p15, we examined the methylation status of the gene in a human GM-CSF-dependent megakaryoblastic leukemic cell line, MO7_c, using bisulfite genomic sequencing. The process of p15 CpG island methylation induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) was investigated, using MO7_c cells. The cells proliferating in response to GM-CSF plus fetal bovine serum (FBS) were almost fully methylated in the p15 CpG island. The withdrawal of both GM-CSF and IBS for 48 hours reduced the cell viability, and increased the frequency of alleles with completely or partially demethylated CpG sites by approximately 50%. Viable cells were responsible for this epigenetic change. The add-back of GM-CSF restored the methylation. Seventy-two hours withdrawal of GM-CSF plus FBS followed by 24 hours exposure to inhibitors for DNA methyltransferase (DNMT) and histone deacctylase (HDAC) ca … More used the demethylation of nearly all CpG sites in the p15 CpG island on every allele sequenced. When GM-CSF was re-added after the 96-hour treatment, the cells exhibited p15 transcriptional silencing via the methylation. The initial methylation event encompassed the entire CpG island. No new methylated alleles appeared in the coexistence of the DNMT and HDAC inhibitors. Taken together, GM-CSF may be able to induce de novo methylation of the p15 gene, using 11DAC(s) as well as DNMT(s).
Histone modifications regulating expression of the genes which encode CDK inhibitors in normal peripheral blood/bone marrow cells and acute myeloblastic leukemia (AML) remain unclear. We attempted, for the first time, to elucidate histone modifications around the CpG island region of the p15 gene in AML cells, using the chromatin immunoprecipitation (ChIP) assay and methylation-specific PCR with bisulfite genomic sequencing. Seven of 11 patients with AML had allele(s) in which more than half of 27 CpG sites in the p15 gene were methylated. The p15 CpG island region was surrounded with both the acetylated histone H3 (A_cH3) and dimethylated histone H3-lysine 9 (M_eH3K9) in bone marrow cells of AML patients, whereas with A_cH3 alone in normal marrow cells. The p 15 CpG islands of DNA immunoprecipitated with anti-A_cH3 antibody and anti-M_eH3K9 antibody were not always unmethylated and methylated, respectively, in the patients. These results suggest perturbed modifications of histone H3 around the p15 CpG island region in AML. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (56 results)

All 2008 2007 2006 2005 Other

All Journal Article (30 results) (of which Peer Reviewed: 23 results) Presentation (26 results)

  • [Journal Article] Recent advances in the pathogenesis and management of juvenile mvelomonocytic leukemia.2008

    • Author(s)
      Koike K. Matsuda K.
    • Journal Title

      Br J Haematol. 141(In press)

      Pages: 567-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Anakinra improved sensory deafness in a Japanese patient with Muckle-Wells syndrome by Possibly inhibiting cryopyrininflammasome.2008

    • Author(s)
      Yamazaki T, Masumoto J, Agematsu K, Sawai N, Kobayashi S, Shigemura T, Yasui K, Koike K.
    • Journal Title

      Arthritis Rheum 2007 58(In press)

      Pages: 864-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Neurodegenerative central nervous system disease as late sequelae of Langerhans cell histiocytosis.2008

    • Author(s)
      Imashuku S, Koike K, 他12名, 4番目.
    • Journal Title

      Haematologica, 93(in press.)

      Pages: 615-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Acquirement of loss of the wild-type NRAS locus with aggres sive disease progression in a patient with juvenile myelomonocytic leukemia and a heterozygous NRAS mutation.2007

    • Author(s)
      Matsuda K, Nakazawa Y. Sakashita K, Shiohara M, Yamauchi K, Koike K.
    • Journal Title

      Haematologica. 92

      Pages: 1576-1578

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Treatment of neurodegenerative CNS disease in langerhans cell histiocytosis with a combination of intravenous immunoglobulin and chemotherapy. Pediatr2007

    • Author(s)
      Imashuku S, Okazaki NA, Nakayama M, Fujita N, Fukuyama T, Koike K, Minato T, Kobayashi R, Morimoto A
    • Journal Title

      Blood Cancer 50

      Pages: 308-11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Spontaneous improvement of hematologic abnormalities in patients having juvenile myelomonocytic leukemia with specific RAS mutations2007

    • Author(s)
      Matsuda K, Shimada A, Yoshida N. Ogawa A, Watanabe A, Yajima S, Iizuka S, Koike K, Yanai F, Kawasaki K, Yanagimachi M, Kikuchi A, Ohtsuka Y, Hidaka E, Yamauchi K, Tanaka M, Yanagisawa R, Nakazawa Y, Shiohara M, Manabe A. Kojima S, Koike K.
    • Journal Title

      blood, 109

      Pages: 5477-80

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A complex karyotype. including a three-way translocation generating a NUP98-HOXD13 transcript. in an infant with acute myeloid leukemia.2007

    • Author(s)
      Hidaka E, Tanaka M, Matsuda K, Ishikawa-Matsumura M, Yamauchi K, Sano K, Honda T, Wakui K, Yanagisawa R, Nakazawa Y, Sakashita K, Shiohara M, Ishii E, Koike K.
    • Journal Title

      Cancer Genet Cytogenet. 176

      Pages: 137-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Late-onset cases of familial hemophagocytic lymphohistiocytosis with missense perforin gene mutations.2007

    • Author(s)
      Ueda I, Kurokawa Y, Koike K, Ito S, Sakata A, Matsumora T, Fukushima T, Morimoto A, Ishii E, Imashuku S.
    • Journal Title

      Am J Hematol 82

      Pages: 427-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analysis of histone modification around the CpG island region of the pl5 gene in acute myeloblastic leukemia2007

    • Author(s)
      Ogawa M, Sakashita K, Zhao XY, Hayakawa A, Kubota T, Koike K
    • Journal Title

      Leukemia Res 31

      Pages: 611-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A possible role for the production of multiple HLA antibodies in fatal platelet transfusion refractoriness after peripheral blood stem cell transplantation from the mother in a patient with relapsed leukemia2007

    • Author(s)
      Nakazawa Y, Saito S, Hasegawa Y, Yanagisawa Y, Sakashita K, Kamijo T, Miyazaki T, Sato S, Ikeda H, Ikebuchi K, Koike K
    • Journal Title

      Transfusio 47

      Pages: 326-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Acquirement of loss of the wild-type NRAS locus with aggressive disease progression in a patient. with juvenile myelomonocytic leukemia and a heterozygous NRAS mutation.2007

    • Author(s)
      Matsuda K. Nakazawa Y. Sakashita K. Shiohara M. Yamauchi K. Koike K.
    • Journal Title

      Haematologica. 92

      Pages: 1576-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Treatment of neurodegenerative CNS disease in langerhans cellhistiocytosis with a combination of intravenous immunoglobulin and chemotherapy2007

    • Author(s)
      Imashuku S, Okazaki NA, Nakayama M, Fujita N, Fukuyama T. Koike K. Minato T, Kobayashi R, Morimoto A.
    • Journal Title

      Pediatr Blood Cancer. 50

      Pages: 308-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous improvement of hematologic abnormalities in patients having juvenile myelomonocytic leukemia with specific RAS mutations.2007

    • Author(s)
      Matsuda K, Shimada A, Yoshida N, Ogawa A, Watanabe A, Yajima S, Iizuka S, Koike K, Yanai F, Kawasaki K, Yanagimachi M, Kikuchi A, Ohtsuka Y, Hidaka E, Yamauchi K, Tanaka M, Yanagisawa R, Nakazawa Y, Shiohara M, Manabe A, Kojima S, Koike K.
    • Journal Title

      Blood 109

      Pages: 5477-80

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A complex karyotype, including a three-way translocation generating a NUP98-HOXD13 transcript, in an infant with acute myeloid leukemia.2007

    • Author(s)
      Hidaka E, Tanaka M, Matsuda K, Ishikawa-Matsumura M, Yamauchi K, Sano K, Honda T, Wakui K, Yanagisawa R, Nakazawa Y, Sakashita K, Shiohara M, Ishii E, Koike K.
    • Journal Title

      Cancer Genet Cytogenet 176

      Pages: 137-43

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Late-onset cases of familial hemophagocytic lymphohistiocytosis with missense perforin gene mutations.2007

    • Author(s)
      Ueda I, Kurokawa Y, Koike K, Ito S, Sakata A, Matsumora T, Fukushima T, Morimoto A, Ishii E, Imashuku S.
    • Journal Title

      Am J Hematol. 82

      Pages: 427-32

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of histone modification around the CpG island region of the p15 gene in acute myeloblastic leukemia.2007

    • Author(s)
      Ogawa M. Sakashita K. Zhao XY. Hayakawa A. Kubota T. Koile K
    • Journal Title

      Leukemia Res 31

      Pages: 611-21

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A possible role for the production of multiple HLA antibodies in fatal platelet transfusion refractoriness after peripheral blood stem cell transplantation from the mother in a patient with relapsed leukemia2007

    • Author(s)
      Nakazawa Y, Saito S. Hasegawa Y. Yanagisawa Y. Sakashita K. Kamijo T. Miyazaki T. Sato S. Ikeda H. Ikebuchi K. Koike K
    • Journal Title

      Transfusion 47

      Pages: 326-34

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromosomal change during 6-mercaptopurine(6-MP)therapy in juvenile myelomonocytic leukemia: the growth of a 6-MP-refractory clone that already exists at onset.2006

    • Author(s)
      Matsuda K, Matsuzaki S, Miki J, Hidaka E, Yanagisawa R, Nakazawa Y, Sakashita K, Kamijo T, Asami K, Sano K, Koike K
    • Journal Title

      Leukemia 20

      Pages: 485-490

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Allogeneic haematopoietic cell transplantation from alternative donors with a conditioning regimen of low-dose irradiation. fludarabine and cyclophosphamide in Fanconi anaemia.2006

    • Author(s)
      Yabe H. Inoue H. Matsumoto M. Hamanoue S. Koike T. Ishiguro H. Koike H. Suzuki K, Kato S. Kojima S. Tsuchida M. Mori T. Adachi S. Tsuji K. Koike K. Morimoto A. Sako M. Yabe M
    • Journal Title

      Br J Haematol. 134

      Pages: 208-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Chromosomal change during 6-mercaptopurine (6-MP) therapy in juvenile myelomonocytic leukemia : the growth of a 6-MP-refractory clone that already exists at onset.2006

    • Author(s)
      Matsuda K, Matsuzaki S, Miki J, Hidaka E, Yanagisawa R, Nakazawa Y, Sakashita K, Kamijo T, Asami K, Sano K, Koike K
    • Journal Title

      Leukemia 20

      Pages: 485-485

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Allogeneic haematopoietic cell transplantation from alternative donors with a conditioning regimen of low-dose irradiation, fludarabine and cyclophosphamide in Fanconi anaemia.2006

    • Author(s)
      Yabe H, Inoue H, Matsumoto M, Hamanoue S, Koike T, Ishiguro H, Koike H, Suzuki K, Kato S, Kojima S, Tsuchida M, Mori T, Adahi S, Tsuji K, Koike K, Morimoto A, Sako M, Yabe M
    • Journal Title

      Br J Haematol 134

      Pages: 208-208

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic subtypes of familial hemophagocytic lymphohistiocytosis: correlations with clinical features and cytotoxic T lymphocyte/natural killer cell functions2005

    • Author(s)
      Ishii E, Ueda I, Shirakawa R, Yamamoto K, Morimoto A, Imayoshi M, Ogata Y, Zaitsu M, Sako M, Koike K, Sakata A, Takada H, Hara T, Imashuku S, Sasazuki T, Yasukawa M.
    • Journal Title

      Blood 105

      Pages: 3442-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Granulocyte-macrophage colony-stimulating factor induces de novo methylation of the p15 CpG island in hematopoietic cells2005

    • Author(s)
      Zhao XY, Sakashita K, Kamijo T, Hidaka E, Sugane, K, Kubota T, Koike K
    • Journal Title

      Cytokine, 31

      Pages: 203-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of two cytogenetically abnormal clones from multipotential hematopoietic stem cells in a patient with juvenile myelomonocytic leukemia.2005

    • Author(s)
      Matsuzaki S. Matsuda K, Miki J, Nakazawa Y, Sakashita K, Kamijo T, Hidaka E, Koike K
    • Journal Title

      Leukemia Res, 29

      Pages: 1069-1072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Methylation status of the ρ15 and ρ16 genes in paediatric myelodysplastic syndrome and juvenile myelomonocytic leukaemia2005

    • Author(s)
      Hasegawa D, Manabe A, Kubota T, Kawasaki H, Hirose I, Ohtsuka Y, Tsuruta T, Ebihara Y, Goto Y, Zhao XY. Sakashita K, Koike K, Isomura M, Kojima S, Hoshika A, Tsuji K, and Nakahata T
    • Journal Title

      Br J Heamatol 128

      Pages: 805-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Granulocyte-macrophage colony-stimulating factor induces de novo methylation of the p15 CpG island in hematopoietic cells2005

    • Author(s)
      Zhao XY, Sakashita K, Kamijo T, Hidaka E, Sugane K, Kubota T, Koike K
    • Journal Title

      Cytokine 31

      Pages: 203-212

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of two cytogenetically abnormal clones from multipotential hematopoietic stem cells in a patient with juvenile myelomonocytic leukemia.2005

    • Author(s)
      Matsuzaki S, Matsuda K, Miki J, Nakazawa Y, Sakashita K, Kamijo T, Hidaka E, Koike K
    • Journal Title

      Leukemia Res 29

      Pages: 1069-1072

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Methylation status of the p15 and p16 genes in paediatric myelodysplastic syndrome and juvenile myelomonocytic leukaemia.2005

    • Author(s)
      Hasegawa D, Manabe A, Kubota T, Kawasaki H, Hirose I, Ohtsuka Y, Tsuruta T, Ebihara Y, Goto Y, Zhao XY, Sakashita K, Koike K, Isomura M, Kojima S, Hoshika A, Tsuji K, Nakahata T
    • Journal Title

      Br J Haematol 128

      Pages: 805-812

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic subtypes of familial hemophagocytic lymphohistiocytosis : correlations with clinical features and cytotoxic T lymphocyte/natural killer cell functions.2005

    • Author(s)
      Ishii E, Ueda I, Shirakawa R, Yamamoto K, Horiuchi H, Ohga S, Furuno K, Morimoto A, Imayoshi M, Ogata Y, Zaitsu M, Sako M, Koike K. Sakata A, Takada H, Hara T, Imashuku S, Sasazuki T, Yasukawa M.
    • Journal Title

      Blood 105

      Pages: 3442-3448

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Analysis of histone modification around the CpG island region of the p15 gene in acute myeloblastic leukemia

    • Author(s)
      Ogawa M, Sakashita K, Zhao XY, Hayakawa A, Kubota T, Koike K
    • Journal Title

      Leukemia Res (印刷中)(未定)

    • Related Report
      2006 Annual Research Report
  • [Presentation] Recent advances in the pathogenesis and management of juvenile myelomonocytic leukemia2008

    • Author(s)
      Koike, K., Matsuda, K
    • Organizer
      Br J Haematol
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Anakinra improved sensory deafness in a Japanese patient with Muckle-Wells syndrome by possibly inhibiting cryopyrin inflammasome2008

    • Author(s)
      Yamazaki, T., Masumoto, J., Agematsu, K., Sawai, N., Kobayashi, S., Shigemura, T., YasuiK, Koike, K
    • Organizer
      Arthritis Rheum 2007
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Neurodegenerative central nervous system disease as late sequelae of Langerhans cell histiocytosis2008

    • Author(s)
      Imashuku, S., Koike, K
    • Organizer
      Haematologica
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] t(8;21)急性骨髄性白血病に対する化学療法および同種骨髄移植後の微少残存病変の解析2007

    • Author(s)
      田中美幸、柳沢 龍、中沢洋三、塩原正明、小池健一
    • Organizer
      第110回日本小児科学会学術集会
    • Place of Presentation
      京都市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 高IgMを合併したSLEの一例2007

    • Author(s)
      山崎崇志、日高義彦、重村倫成、石田岳史、上松一永、江原孝史、小池健一
    • Organizer
      第29回日本小児腎不全学会総会
    • Place of Presentation
      松江市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 腸管型ベーチェット病を併発しtrisomy 8とPTPN11遺伝子異常を認めた骨髄異形成症候群(RAEB-M6)の1例2007

    • Author(s)
      重村倫成、高山和生、齋藤章治、田中美幸、柳沢 龍、坂下一夫、山崎崇志、塩原正明、上松一永、小池健一、松田和之、樋口由美子
    • Organizer
      第49回日本小児血液学会
    • Place of Presentation
      仙台市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Acquirement of loss of the wild-type NRASlocus with aggressive disease progression in a patient with juvenile myelomonocytic leukemia and a heterozygous NRAS mutation2007

    • Author(s)
      Matsuda, K., Nakazawa, Y., Sakashita, K., Shiohara, M., Yamauchi, K., Koike, K
    • Organizer
      Pediatr Blood Cancer
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Spontaneous improvement of hematologic abnormalities in patients having juvenile myelomonocytic leukemia with specific RAS mutations2007

    • Author(s)
      Matsuda, K., Shimada, A., Yoshida, N., Ogawa, A., Watanabe, A., Yajima, S., Iizuka, S., Koike, K., Yanai, F., Kawasaki, K., Yanagimachi, M., Kikuchi, A., Ohtsuka, Y., Hidaka, E., Yamauchi, K., Tanaka, M., Yanagisawa, R., Nakazawa, Y., Shiohara, M., Manabe, A., Kojima, S., Koike, K
    • Organizer
      Blood
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A complex karyotype, including a three-way translocation generating a NUP98-HOXD13 transcript, in an infant with acute myeloid leukemia2007

    • Author(s)
      Hidaka, E., Tanaka, M., Matsuda, K., Ishilawa-Matsumura, M., Yamauchi, K., Sano, K., Honda, T., Wakui, K., Yanagisawa, R., Nakazawa, Y., Sakashita, K., Shiohara, M., Ishii, E., Koike, K
    • Organizer
      Cancer Genet Cytogenet
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Late-onset cases of familial hemophagocytic lymphohistiocytosis with missense perforin gene mutations.Am J Hematol2007

    • Author(s)
      Ueda, I., Kurokawa, Y., Koike, K., Ito, S., Sakata, A., Matsumora, T., Fukushima, T., Morimoto, A., Ishii, E., Imashuku, S
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of histone modification around the CpG island region of the p 15 gene in acute myeloblastic leukemia2007

    • Author(s)
      Ogawa, M., Sakashita, K., Zhao, XY., Hayakawa, A., Kubota, T., Koike, K
    • Organizer
      Leukemia Res
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A possible role for the production of multiple HLA antibodies in fatal platelet transfusion refractoriness after peripheral blood stem cell transplantation from the mother in a patient with relapsed leukemia2007

    • Author(s)
      Nakazawa, Y., Saito, S., Hasegawa, Y., Yanagisawa, Y., Sakashita, K., Kamijo, T., Miyazaki, T., Sato, S., Ikeda, H., Ikebuchi, K., Koike, K
    • Organizer
      Transfusion
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 造血機構の解析と臨床への応用2007

    • Author(s)
      小池 健一
    • Organizer
      第110回日本小児科学会学術集会
    • Place of Presentation
      京都市
    • Related Report
      2007 Annual Research Report
  • [Presentation] ★t(8;21)急性骨髄性白血病に対する化学療法および同種骨髄移植後の微少残存病変の解析2007

    • Author(s)
      田中美幸、柳沢 龍、中沢洋三、塩原正明、小池健一
    • Organizer
      第110回日本小児科学会学術集会
    • Place of Presentation
      京都市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 治療に難渋している抗Jo-1抗体陽性の多発性筋炎2007

    • Author(s)
      重村倫成、山崎崇志、上松一永、小池健一
    • Organizer
      第110回日本小児科学会学術集会
    • Place of Presentation
      京都市
    • Related Report
      2007 Annual Research Report
  • [Presentation] 小児科領域におけるITの活用2006

    • Author(s)
      小池 健一
    • Organizer
      第109回日本小児科学会学術集会
    • Place of Presentation
      金沢市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 全身放射線照射量を減量した前処置による造血幹細胞移植の治療成績2006

    • Author(s)
      柳沢 龍、田中美幸、中沢洋三、塩原正明、上條岳彦、小池健一
    • Organizer
      第109回日本小児科学会学術集会
    • Place of Presentation
      金沢市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 急性骨髄性白血病に対する同種骨髄移植後のAML1-ETOキメラ遺伝子による微少残存病変の解析2006

    • Author(s)
      田中美幸、柳沢龍、中沢洋三、塩原正明、小池健一、松田和之、鈴木剛史、川久保雅友
    • Organizer
      第68回日本血液学会・第48回日本臨床血液学会
    • Place of Presentation
      福岡市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Chromosomal change during 6-mercaptopurine (6-MP) therapy in juvenile myelomonocytic leukemia : the growth of a 6-MP-refractory clone that already exists at onset2006

    • Author(s)
      Matsuda, K., Matsuzaki, S., Mild, J., Hidaka, E., Yanagisawa, R., Nakazawa, Y., Sakashita, K., Kamijo, T., Asami, K., Sano, K., Koike, K
    • Organizer
      Leukemia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Allogeneic haematopoietic cell transplantation from alternative donors with a conditioning regimen of low-dose irradiation, fludarabine and cyclophosphamide in Fanconi anaemia2006

    • Author(s)
      Yabe, H., Inoue, H., Matsumoto, M., Hamanoue, S., Koike, T., Ishiguro, H., Koike, H., Suzuki, K., Kato, S., Kojima, S., Tsuchida, M., Mod, T., Adachi, S., Tsuji, K., Koike, K., Morimoto, A., Sako, M., Yabe, M
    • Organizer
      Br J Haematol
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 若年性慢性骨髄性白血病におけるp15遺伝子のDNAメチル化とヒストン修飾2005

    • Author(s)
      小川美奈、坂下一夫、小池健一
    • Organizer
      第108回日本小児科学会学術集会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Three-wayおよびfour-way translocationを示した乳児白血病の2例2005

    • Author(s)
      田中美幸, 柳沢龍, 中沢洋三, 塩原正明, 上條岳彦, 小池健一, 松田和之、鈴木剛史、日高恵以子
    • Organizer
      臨床血液学会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic subtypes of familial hemophagocytic lymphohistiocytosis : correlations with clinical features and cytotoxic T lymphocyte/natural killer cell functions2005

    • Author(s)
      Ishii, E., Ueda, I., Shirakawa, R., Yamamoto, K., Horiuchi, H., Ohga, S., Furuno, K., Morimoto, A., Imayoshi, M., Ogata, Y., Zaitsu, M., Sako, M., Koike, K., Sakata, A., Takada, H., Hara, T., Imashuku, S., Sasazuki, T., Yasukawa, M
    • Organizer
      Blood
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Granulocyte-macrophage colony-stimulating factor induces de novo methylation of the p15 CpG island in hematopoietic cells2005

    • Author(s)
      Zhao, XY., Sakashita, K., Kamijo, T., Hidaka, E., Sugane, K., Kubota, T., Koike, K
    • Organizer
      Cytokine
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of two cytogenetically abnormal clones from multipotential hematopoietic stem cells in a patient with juvenile myelomonocytic leukemia2005

    • Author(s)
      Matsuzaki, S., Matsuda, K., Miki, J., Nakazawa, Y., Sakashita, K., Kamijo, T., Hidaka, E., Koike, K
    • Organizer
      Leukemia Res
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Methylation status of the p15 and p16 genes in paediatric myelodysplastic syndrome and juvenile myelomonocytic leukaemia2005

    • Author(s)
      Hasegawa, D., Manabe, A., Kubota, T., Kawasaki, H., Hirose, I., Ohtsuka, Y., Tsuruta, T., Ebihara, Y., Goto, Y., Zhao, XY., Sakashita, K., Koike, K., Isomura, M., Kojima, S.,Hoshika, A., Tsuji, K., Nakahata, T
    • Organizer
      Br J Heamatol
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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