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Studies on radiopharmaceuticals with mitochondria membrane potential dependent tumor accumulation

Research Project

Project/Area Number 17390330
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

SAKAHARA Harumi  Hamamatsu University School of Medicine, Faculty of Medicine, Department of Radiology, Professor (10187031)

Co-Investigator(Kenkyū-buntansha) MAGATA Yasuhiro  Hamamatsu University School of Medicine, Photon Medical Research Center, Laboratory of Genome Bio-Photonics, Professor (20209399)
OGAWA Mikako  Hamamatsu University School of Medicine, Photon Medical Research Center, Laboratory of Genome Bio-Photonics, Assistant Professor (20344351)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥9,530,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥630,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsTumor / Nuclear Medicine / PET / SPECT / Membrane Potential / adiopharmaceuticals
Research Abstract

It is well known that lipophilic cation radiopharmaceuticals such as T1-201 or Tc-99m-MIBI (MIBI) accumulate in some tumors and they are useful for tumor diagnosis. Both T1-201 and MIBI are currently used for myocardial blood flow scintigraphy. The uptake mechanism and kinetics in myocaridium cells have been reported to be different in these two radiopharmaceuticals. According to these reports, almost MIBI in the cells exist in the mitochondria fraction and the uptake of MIBI by myocardium cells depends on mitochondria membrane potential. Actually, the membrane potential of the myocardium is higher than that of other organs. In addition, the mitochondria membrane potential of some tumor cells is higher than that of normal cells_ It is expected that MIBI accumulates in the tumor cells depending on the membrane potential. On these basis, the uptake kinetics and patho-physiological studies of such cationic radiopharmaceuticals in the tumor cells was evaluated using in vitro cell culture e … More xperimental system in this research.
First of all, we prepared some tumor cell lines for in vitro experiments. Relative mitochondria membrane potential of these cells was evaluated with JC-1 and a large variation of the membrane potential was indicated. In in vitro experiments, a high relationship between the membrane potential and MIBI uptake in the tumor cells was observed. In addition, similar relationship was shown in case of FDG. Because mitochondria membrane potential relates to the activity of energy metabolism, these results suggested that the uptake of both compounds can be used as index of the tumor cells activity. Next, we perforthed MIBI and FDG uptake studies in the tumor cells after photo dynamic therapy (PDT). About 50 % decrease per cell at 1-3 hours post PDT irradiation was observed in only MIBI uptake. It is suggested this phenomenon is derived from the alteration of mitochondria function because of apoptosis induced by PDT. This discrepancy between MIBI and FDG uptake interests us for diagnosis of tumor cell condition after therapy. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (2 results)

  • [Journal Article] Visualization of nasolacrimal drainage system after radioiodine therapy in patients with thyroid cancer.2007

    • Author(s)
      Sakahara H
    • Journal Title

      Ann Nucl Med 21(9)

      Pages: 525-527

    • NAID

      10020001112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Visualization of nasolacrimal drainage system after radioiodine therapy in patients with thyroid cancer2007

    • Author(s)
      Sakahara, H., et. al.
    • Journal Title

      Ann Nucl Med 21(9)

      Pages: 525-527

    • NAID

      10020001112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Visualization of nasolacrimal drainage system after radioiodine therapy in patients with thyroid cancer2007

    • Author(s)
      Sakahara H
    • Journal Title

      Ann Nucl Med 21

      Pages: 525-527

    • NAID

      10020001112

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Applicability of ahigh-resolution small semiconductor gamma camera to small animal imaging2007

    • Author(s)
      Kiyono Y
    • Journal Title

      Nucl Med Biol 23

      Pages: 736-741

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rhemium-186-monoaminemonoamidedithiol-conjugated bisphosphonate derivatives for bone pain palliation.2006

    • Author(s)
      Ogawa K
    • Journal Title

      Nucl Med Biol 33(4)

      Pages: 513-520

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of hemodynamics in a silicon aneurysm model using time-resolved, 3D, phase-contrast MRI.2006

    • Author(s)
      Isoda H
    • Journal Title

      AJNR Am J Neuroradiol 27(5)

      Pages: 1119-1122

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rhemium-186-monoaminemonoamidedithiol-conjugated bisphosphonate derivatives for bone pain palliation2006

    • Author(s)
      Ogawa, K., et. al.
    • Journal Title

      Nucl Med Biol 33(4)

      Pages: 613-520

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Visualization of hemodynamics in a silicon aneurysm model using time-resolved, 3D, phase-contrast MRI2006

    • Author(s)
      Isoda, H., et. al.
    • Journal Title

      AJNR Am J Neuroradiol 27(5)

      Pages: 1119-1122

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Application of 18F-FDG PET for monitoring the therapeutic effect of antiinflammatory drugs on stabilization of vulnerable atherosclerotic plaques.2006

    • Author(s)
      Ogawa M
    • Journal Title

      J Nucl Med 47(11)

      Pages: 1845-1850

    • Related Report
      2006 Annual Research Report
  • [Presentation] こころとからだの分子が見える2007

    • Author(s)
      間賀田泰寛
    • Organizer
      第12回静岡健康・長寿学術フォーラム
    • Place of Presentation
      静岡
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vivo imaging to explore living human body2007

    • Author(s)
      Yasuhiro, Magata
    • Organizer
      The 12th Shizuoka Forum on Health and Longevity
    • Place of Presentation
      Shizuoka Japan
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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