Development of isoform-specific amino acid transporter imaging agent
| Project/Area Number |
17390336
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Ibaraki Prefectural University of Health Science |
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Principal Investigator |
SHIKANO Naoto Ibaraki Prefectural University of Health Science, Department of Radiological Sciences, Assistant Professor (80295435)
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| Co-Investigator(Kenkyū-buntansha) |
SHIKAWA Nobuyoshi Ibaraki Prefectural University of Health Sciences, Department of Radiological Sciences, Professor (10026932)
KAWAI Keiichi Kanazawa University, Faculty of Medicine, Professor (30204663)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥8,940,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | radiopharamaceutical / artificial amino acid / L-type amino acid transporter / 4F2hc / neutral amino acid / functional imaging / system L / isoform selectivity / 放射性医薬品 / 人工アミノ酸 / アミノ酸輸送 / 機能診断薬 / 画像診断薬 / L系 / アイソフォーム選択性 |
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| Research Abstract |
Human L-type amino acid transporter 1 (hLAT1), a component of system L, is expressed in a wide variety of organs. Its highest expression levels are in the brain, placenta, testis, bone marrow and fetal liver, followed by peripheral leukocytes, lymph nodes and the thymus. In a previous study, LAT1 was found to act as a system L transporter at the blood-brain barrier and tumor tissue. On the other hand, hLAT2, an isoform of hLATi, involves in the trans-cellular transport of neutral amino acids in epithelia blood-tissue barriers. Development of isoform-specific amino acid transporter agent is helpful about clarification of amino acid metabolic disease. We examined system-selectivity of 3-[^ (125) I]iodo-α-methyl-L-tyrosine (^<125>IMT), 4-[^<125>I]iodo-L-meta-tyrosine (4-[^<125>I]-mTyr) and 3-[^<125>I]iodo-L-tyrosine (3-[^<125>I]-Tyr) transport in Chinese hamster ovary cell K1 (CHO-K1) and those isoform-selectivity of the two human L-type amino acid transporters, hLAT1 and hLAT2 with human 4F2hc co-expressed Xenopus laevis oocytes. CHO-Klcell line studies have revealed that 4-[^<125>I]-mTyr exhibited the greatest system L specificity (93.46±0.13%) of all the amino acids tested, and therefore shows promise as a system-L-selective amino acid transport marker. [^<125>I]IMT, 4-[^<125>I]-mTyr and 3- [^<125>I] -Tyr transport was hLAT1-selective. Animal studies with these compounds are anticipated in further studies.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Pharmacokinetics of 3-[^<125>I]iodo-alpha-methyl-L-tyrosine, a tumor imaging agent, after probenecid loading in mice implanted with colon cancer DLD-1 cells2007
Author(s)
S. Nakajima, N. Shikano, T. Kotani, M. Ogura, R. Nishii, M. Yoshimoto, N. Yamaguchi, Y. Iwamura, N. Kubota, N. Ishikawa, K. Kawai
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Journal Title
Nucl Med Biol 34
Pages: 1003-1008
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Fluorescent X-ray imaging with synchrotron radiation for the evaluation of probes for biomedical functions : A pilot study on 4-bromo-DL-phenylalanine for cancer detection in mice2007
Author(s)
N. Shikano, T. Kotani, S. Nakajima, M. Ogura, K. Hyodo, H. Sugiyama, M. Monma, K. Kawai, T. Takeda, N. Kubota, K. Nishimura, K. Mori, N. Ishikawa
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Journal Title
NAID
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Assessment of D-[1-^<14>C]-amino acids transport using Chinese hamster ovary cells : implications for use of labeled D-amino acids as molecular imaging agents2007
Author(s)
N. Shikano, N. Nakajima, T. Kotani, M. Ogura, R. Nishii, K. Nishi, M. Yoshimoto, N. Kubota, N. Ishikawa, K. Kawai
Organizer
Joint Molecular Imaging Conference
Place of Presentation
Providence, Rhode Island USA
Description
「研究成果報告書概要(欧文)」より
Related Report
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