| Project/Area Number |
17390352
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
TAGUCHI Tomoaki Kyushu University, Graduate School of Medical Sciences, Professor (20197247)
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| Co-Investigator(Kenkyū-buntansha) |
SUITA Sachiyo Kyushu University, Hospital, Professor (30038856)
NISHIMOTO Yuko Kyushu University, Hospital, Assistant Professor (00419574)
MATSUZAKI Akinobu Kyushu University, Graduate School of Medical Sciences, Professor (90238999)
SUMINOE Aiko Kyushu University, Hospital, Assistant Professor (80335968)
TAJIRI Tatsuro Kyushu University, Graduate School of Medical Sciences, Associate Professor (80304806)
荻田 桂子 九州大学, 大学院・医学研究院, 助手 (40346771)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,620,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | small intestinal transplantation / non-myeloablating stem cell transplantation / chimerism / immunotolerance / MHC / large animal / miniature swine / living-related transplantation |
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| Research Abstract |
Survival rate after Small intestinal transplantation has been improved gradually in recent 10 years. However, incidence of acute as well as chronic rejection is still high, and late graft loss is sometimes happen. In order to overcome immunological obstacles, induction of tolerance is called for.
Non-myeloablating stem cell transplantation has been introduced as treatment tool for leukemia. In living-related organ transplantation, donor specific tolerance can be induced by this method. After tolerance induction by stem cell transplantation, small intestinal graft from the same donor can be accepted without immunosuppression. We have starting to establish this method using mini-swine model of small intestinal transplantation.
In the first year, operion technique of small intestinal transplantation, using miniature swine was established. Ileal graft was harvested with pedicle. Cutting site of each superior mesenteric vessel was radix of celiac trunks and portal vein. Then graft was anastosed to Aorta and graft vein was anastomosed to IVC by end-to-side fashion.
In the second year, non-myeloablating stem cell transplantation was started. Bone marrow cells were harvested from iliac bone. However the number of cells were not enough for tstem cell transplantation. Then we started irrigation of bone marrow, using two needle. After that the number of cells were increased. The numbers were identified by CD34 immunostaining.
In the third year, miniature swine, SLA established syngenic chrone, cannot be supplied by animal farm. Especially, combination of SLA 1 mismatch is impossible now. We have proposed the protochol of chemotherapy which can establish the non-myelloablating protochol.
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