| Project/Area Number |
17390378
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Mie University |
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Principal Investigator |
SHIMONO Takatsugu Mie University, Graduate School of Medicine, Associate Professor (80206242)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Noriyuki Mie University, Graduate School of Medicine, Associate Professor (40214390)
MIYAMOTO Keigi Mie University, Graduate School of Engineering, Associate Professor (70252343)
HIRATA Hitoshi Nagoya University, Graduate School of Medicine, Professor (80173243)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,670,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | Aortic aneurysm / Endovascular surgery / Stent-Graft / Cell transplantation / Fibroblast / Myoblast / Drug Delivery System |
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| Research Abstract |
We demonstrated previously that transplantation of a cell combination(myoblasts and fibroblasts) promoted thrombus organization in a rat model. We also developed basic fibroblast growth factor(bFGF) slow-delivery stent grafts(S/Gs) coated with elastin and impregnated with bFGF. In this project, the effects of cell transplantation combined with bFGF slow release on canine thoracic aortic aneurysmal sacs after S/Gs repair were evaluated.
Thoracic aortic aneurysms were surgically created with jugular vein patches in 15 beagles. Myoblasts and fibroblasts of autologous skeletal muscle were isolated for cell transplantation. The S/Gs with 6 holes were used in this experiment as endoleaking models. Collagen gel alone(control group n=5) or a mixture of skeletal and fibroblasts with collagen gel(cell group n=5) were injected into the aneurysmal sac excluded S/Gs. The combined therapy of bFGF slow-release S/Gs and cell therapy(hybrid group n=5) was also evaluated. After 14 days, histological analyses revealed that the excluded aneurysmal cavities of the control group were filled with fresh thrombus, whereas the excluded cavities in the cell group and the hybrid group were occupied by organized tissue. The percentages of the organized areas relative to the excluded cavities, evaluated by Masson's trichrome staining, were 18.1%, 52.6% and 77.1% in the control, cell, and hybrid groups, respectively. Also we evaluated some bio-gels for the injection into the anurysmal cavities with autolougus cells and concluded that autologus fibrin glue was suitable for this purpose.
Cell transplantations into excluded aneurysmal cavity with bFGF slow-release stent grafts may improve results of endovascular surgery for aortic aneurysms, especially against the type II endoleak
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