| Project/Area Number |
17390389
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
HIDA Kazutoshi Hokkaido University, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (10238305)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Satoshi Hokkaido University, HOSPITAL, LECTURER (10301904)
IWASAKI Yoshinobu Hokkaido Unive., GRADUATE SCHOOL OF MEDICINE, PROFESSOR (00113522)
YANO Shunsuke Hokkaido University, HOSPITAL, ASSOCLATE PROFESSOR (20374481)
小林 浩之 北海道大学, 病院, 助教 (70374478)
中山 若樹 北海道大学, 北海道大学病院, 助手 (40421961)
七戸 秀夫 北海道大学, 病院・医員 (80374479)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,670,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2006: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2005: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Cerebral infarction / Spinal cord injury / Bone marrow stromal cell / Transplantation / Diffuse axonal injury / Differentiation / Regenerative medicine / Functional restoration / Diffuse brain injury / Cognitive function / DNA microarray / Cerebral stroke / Migration / Biomaterial / Neuroprotection |
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| Research Abstract |
We isolated the BMSC from the green fluorescence protein (GFP)-transgenic mice and transplanted them into the mice subjected to spinal cord injury. We could visualize the transplanted cells through the dura mater in the living animals for 4 weeks after transplantation, using a novel fluorescence imaging technique. These data were published in J Neurotrauma (2005). Furthermore, we have confirmed that the transplanted BMSC could improve the GABA receptor function around cerebral infarction and spinal cord injury, using autoradiography and fluorescence immunostaining. These data were published in J Nucl Med (2006) and J Neurotrauma (2007). We also found that the BMSC actively repeat cell proliferation when transplanted into infarct brain, and published the data in Brain Res (2005). We assessed gene expression profile in the cultured BMSC and clarified that the BMSC have the potential to alter their gene expression profile in response to external stimuli. The data were published in Brain Res (2006). We have clarified that the BMSC have the potential to degrade the extracellular matrix in the spinal cord and produce the chemo-attractant factor for axon elongation of the neurons, using a novel "in vitro transplantation" model. The data have been submitted to Neurorehab and Neural Repair. We have also clarified that CXCR4/SDF-1 system is essential in the proliferation and migration of the transplanted BMSC, using the CXCR4-knock out mice. The data have been submitted to Brain Res. Using co-culture paradigm, we have also clarified that the BMSC have the potential to differentiate into the neural cells and protect the neurons exposed to glutamate. The data have been submitted to J Neurosci Res. We have also found that fibrin glue is a suitable biomaterial as a scaffold for the BMSC when transplanted into brain cold injury model or spinal cord hemi-section model of the rats. These data has been prepared for submission to Neurosurgery and J Neurotrauma.
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