Induction of dopaminergic neurons from human ES cells, and transplantation of the cells into monkey Parkinson's disease models
| Project/Area Number |
17390397
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
TAKAHASHI Jun Kyoto University, Institute for Frontier Medical Sciences, associate professor (10270779)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKATSUJI Norio KYOTO UNIVERSITYKYOTO UNIVERSITY, Institute for Frontier Medical Scienoes, professor (80237312)
SASAI Yoshiki Riken Kobe Institute, CDB, Group Director (20283616)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥16,750,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000)
|
|---|
| Keywords | embryonic stem cells / neural induction / cell transplantation / Parkinson's disease / dopaminergic neuron / tumor formation / ヒトES細胞 / 神経細胞移植 |
|---|
| Research Abstract |
We induced dopaminergic neurons from human ES cell lines, KhES-1, -2 -3, which were established in Kyoto University, Japan-When we grafted human ES cell-derived neural progenitors into the striatum of monkey Parkinson's disease, one of the three monkeys showed tumor formation. PET scan demonstrated an increase of glucose metabolism and an uptake of fluorothimidine at the tumor region. Immunofluorescence study showed accumulation of Oct3/4-, ES cell marker positive cells in the tumor. In to analysis of the grafted cells revealed that the cells which caused tumor formation contained Oct3/4-positive ES cells, but that the cells which did not tumors contained no such cells. These results suggest that contaminated ES cells in the graft lead to tumor formation.
This possibility was confirmed by using mouse ES cells. When ES cell-derived neural progenitor cells (NPCs) were selected. by fluorescence-activated cell sorting (FACS), these cells never famed any tumors in the mouse brain or subcutaneous space of immunodeficient mice.
These results indicate that purification of NPCs is important for the safe and effective cell transplantation, and we are now developing the method to sort out human ES cell-derived NPCs.
|
Report
(4 results)
Research Products
(42 results)
-
-
-
[Journal Article] Meningeal cells induce dopaminergic neurons from embryonic stem cells2008
Author(s)
Hayashi, H., Morizane, A., Koyanagi, M., Ono, Y., Sasai, Y., Hashimoto, N., Takahashi, J
-
Journal Title
Eur J Neurosci 27
Pages: 261-268
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors2008
Author(s)
Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., Hashimoto, N
-
Journal Title
J Neurosci Res 86
Pages: 270-280
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
[Journal Article] Generation of graftable progenitors of dopaminergic neurons from mouse ES cells by a combination of co-culture and neurosphere methods2006
Author(s)
Morizane, A., Takahashi, J., Shinoyama, M., Ideguchi, M., Takagi, Y., Fukuda, H., Koyanagi, M., Sasai, Y., Hashimoto, N
-
Journal Title
J Neurosci Res 83
Pages: 1015-1027
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] FACS-based purification of ES cell-derived neural precursors averts tumor formation after transplantation2006
Author(s)
Fukuda, H., Takahashi, J., Watanabe, K., Hayashi, H., Morizane, A., Koyanagi, M., Sasai, Y., Hashimoto, N
-
Journal Title
Stem Cells 24
Pages: 763-771
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Journal Article] Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells
Author(s)
Ideguchi, M., Shinoyama, M., Gomi, M., Hayashi, H., Hashimoto, N., Takahashi, J
-
Journal Title
J Neurosci Res (In press)
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] ES細胞を用いた神経再生医療2006
Author(s)
高橋 淳
Organizer
第9回日本組織工学会シンポジウム
Place of Presentation
京都
Year and Date
2006-09-08
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
