| Project/Area Number |
17390397
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAHASHI Jun Kyoto University, Institute for Frontier Medical Sciences, associate professor (10270779)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUJI Norio KYOTO UNIVERSITYKYOTO UNIVERSITY, Institute for Frontier Medical Scienoes, professor (80237312)
SASAI Yoshiki Riken Kobe Institute, CDB, Group Director (20283616)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,750,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | embryonic stem cells / neural induction / cell transplantation / Parkinson's disease / dopaminergic neuron / tumor formation / ヒトES細胞 / 神経細胞移植 |
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| Research Abstract |
We induced dopaminergic neurons from human ES cell lines, KhES-1, -2 -3, which were established in Kyoto University, Japan-When we grafted human ES cell-derived neural progenitors into the striatum of monkey Parkinson's disease, one of the three monkeys showed tumor formation. PET scan demonstrated an increase of glucose metabolism and an uptake of fluorothimidine at the tumor region. Immunofluorescence study showed accumulation of Oct3/4-, ES cell marker positive cells in the tumor. In to analysis of the grafted cells revealed that the cells which caused tumor formation contained Oct3/4-positive ES cells, but that the cells which did not tumors contained no such cells. These results suggest that contaminated ES cells in the graft lead to tumor formation.
This possibility was confirmed by using mouse ES cells. When ES cell-derived neural progenitor cells (NPCs) were selected. by fluorescence-activated cell sorting (FACS), these cells never famed any tumors in the mouse brain or subcutaneous space of immunodeficient mice.
These results indicate that purification of NPCs is important for the safe and effective cell transplantation, and we are now developing the method to sort out human ES cell-derived NPCs.
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