| Project/Area Number |
17390400
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Okayama University |
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Principal Investigator |
DATE Isao Okayama University, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, PROFESSOR (70236785)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIU Kenji OKAYAMA UNIVERSITY HOSPITALS, 医学部・歯学部附属病院, Senior, Assistan+Professor (40325105)
TOKUNAGA Kouji OKAYAMA UNIVERSITY HOSPITALS, 医学部・歯学部附属病院, Senior, Assistant+Professor (40294467)
MIYOSHI Yasuyuki OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, Senior Assistant Professor (00362997)
ONO Shigeki OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, ASSISTANT PROFESSOR (40335625)
ISHIKAWA Tomotsugu OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE DENTISTRY AND PHARMACEUTICAL SCIENCES, ASSISTANT PROFESSOR (10362964)
小野田 恵介 (小野田 惠介) 岡山大学, 大学院・医歯薬学総合研究科, 助手 (20379837)
松井 利浩 岡山大学, 大学院・医歯薬学総合研究科, 助手 (80362995)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,910,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | neural stem / progenitor cells / encapsulation / Dopamine neuron / Parkinson's disease / cerebral ischemia / neurotrophic factor / カプセル / 細胞外基質 / 腫瘍化 / 分化誘導 |
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| Research Abstract |
After confirmation of the therapeutic effects of encapsulated ES cell- or NT2 cell-derived dopaminergic neurons on Parkinson's diming= model of rats, adult rat-derived neural stem/progenitor cells were focused on. Encapsulated adult rat-derived neural stem/progenitor calls survived for 2 months with or without prospective neuronal differentiation without tumor formation. Then, neural stem/progenitor cells were isolated from adult macaque subventricular zone or hippocampus. The growth speed of the cells was so slow that it took at least one and a half months to he amplified for encapsulation. Type I collagen remarkably ameliorated the survival of encapsulated neural stem/progenitor rolls and the surviving rate of the encapsulated macaque cells were almost the same as that of rat cells. Neuronal differentiation of encapsulated undifferentiated macaque-derived neural stern/progenitor cells was little with much glial differentiation. Encapsulated undifferentiated cells survived for 1 month in a Parkinson's disease model of macaque, although it did not significantly ameliorate abnormal behavior On the other hand, macaque-derived neural stern/progenitor rolls could be encapsulated after neuronal differentiation. Dopaminergic differentiation with subsequent encapsulation resulted in dopamine-secretion from the capsule. Encapsulated differentiated rolls improved the behavioral scores, indicating that encapsulated dopaminergic neurons derived from adult macaque neural stem/progenitor cells had therapeutic effects on a Parkinson's disease model of macaque with no tumor formation by thstiff capsule wall Clinical application might be achievable in the figure, although several preclinical studies remain to be solved.
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