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Analysis of Bone Remodeling using Osteopetrotic and Osteosclerotic Mouse Models

Research Project

Project/Area Number 17390420
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKeio University

Principal Investigator

MATSUO Koichi  Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (40229422)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Toru  Keio University, School of Medicine, Instructor, 医学部, 助手 (50338092)
TAKADA Yasunari  Keio University, School of Medicine, Instructor, 医学部, 助手 (40407086)
TOYAMA Yoshiaki  Keio University, School of Medicine, Professor, 医学部, 教授 (40129549)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2005: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsOsteoimmunology / Bone remodeling / ephrin / c-Fos / Osteoclast / cytokine / OPG / Auditory ossicles
Research Abstract

1. Bone homeostasis requires a delicate balance between the activities of bone-resorbing osteoclasts and bone-forming osteoblasts. Various molecules coordinate osteoclast function with that of osteoblasts; however, molecules that mediate osteoclast-osteoblast interactions by simultaneous signal transduction in both cell types have not yet been identified. Here we show that osteoclasts express the NFATc 1 target gene ephrinB2, while osteoblasts express the receptor EphB4, along with other ephrin-Eph family members. Using gain-and loss-of-function experiments, we demonstrate that reverse signaling through ephrinB2 into osteoclast precursors suppresses osteoclast differentiation by inhibiting the osteoclastogenic c-Fos-NFATc 1 cascade. In addition, forward signaling through EphB4 into osteoblasts enhances osteogenic differentiation, and overexpression of EphB4 in osteoblasts increases bone mass in transgenic mice. These data demonstrate that ephrin-Eph bidirectional signaling links two major molecular mechanisms for cell differentiation-one in osteoclasts and the other in osteoblasts-thereby maintaining bone homeostasis (Zhao et al., 2006).
2. Fral transgenic (Tg) mice develop osteosclerosis and exhibit altered expression of bone matrix proteins. We found that expression of Thbsl and Thbs2 was reduced in Fral Tg osteoblasts. Fral Tg and non-osteosclerotic Thbsl-/-Thbs2-/-mice share an edge-to-edge bite. Therefore, reduced expression of thrombospondins may contribute to craniofacial dysmorphism independently of osteosclerosis (Nishiwaki et al., 2006). We also observed that inflammatory cytokine production is reduced in Fral Tg mice and initiation of osteoblastic differentiation is delayed after bone fracture (manuscript in preparation).

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (17 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Role of heterodimerization of Fos and Fra1 proteins in osteoclast differentiation2007

    • Author(s)
      Larifa Bakiri et al.
    • Journal Title

      Bone 40・4

      Pages: 867-875

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of heterodimerization of Fos and Fra1 proteins in osteoclast differentiation.2007

    • Author(s)
      Bakiri, L., Takada, Y., Radolf, M., Eferl, R., Yaniv, M., Wagner, E.F., Matsuo, K.
    • Journal Title

      Bone 40

      Pages: 867-875

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] c-Fos-deficient mice are susceptible to Salmonella enterica serovar Typhimurium infection.2007

    • Author(s)
      Kenta Maruyama et al.
    • Journal Title

      Infection and Immunity 75・3

      Pages: 1520-1523

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reduced expression of thrombospondins and craniofacial dysmorphism in mice overexpressing Fral2006

    • Author(s)
      Toru Nishiwaki et al.
    • Journal Title

      Journal of Bone and Mineral Research 21・4

      Pages: 596-604

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Resorption of auditory ossicles and hearing loss in mice lacking osteoprotegerin.2006

    • Author(s)
      Sho Kanzaki et al.
    • Journal Title

      Bone 39・2

      Pages: 414-419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis.2006

    • Author(s)
      Chen Zhao et al.
    • Journal Title

      Cell Metabolism 4・2

      Pages: 111-121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reduced expression of thrombospondins and craniofacial dysmorphism in mice overexpressing Fra1.2006

    • Author(s)
      Nishiwaki, T., Yamaguchi, T., Zhao, C., Amano, H., Hankenson, K.D., Bornstein, P., Toyama, Y., Matsuo, K.
    • Journal Title

      Journal of Bone and Mineral Research 21

      Pages: 596-604

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Resorption of auditory ossicles and hearing loss in mice lacking osteoprotegerin.2006

    • Author(s)
      Kanzaki, S., Ito, M., Takada, Y., Ogawa, K., Matsuo.K.
    • Journal Title

      Bone 39

      Pages: 414-419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis.2006

    • Author(s)
      Zhao, C., lrie, N., Takada, Y., Shimoda, K., Miyamoto, T., Nishiwaki, T., Suda, T., Matsuo, K.
    • Journal Title

      Cell Metabolism 4

      Pages: 111-121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] c-Fos suppresses systemic inflammatory response to endotoxin2006

    • Author(s)
      Neelanjan Ray et al.
    • Journal Title

      International Immunology 18・5

      Pages: 671-677

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bidirectional ephiinB2-EphB4 signaling controls bone homeostasis.2006

    • Author(s)
      Chen Zhao et al.
    • Journal Title

      Cell Metabolism 4・2

      Pages: 111-121

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Receptor activator of NF-kappa B ligand and osteoprotegerin regulate proinf lammatory cytokine production in mice.2006

    • Author(s)
      Kenta Maruyama et al.
    • Journal Title

      Journal of Immunology 177・6

      Pages: 3799-3805

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reduced expression of thrombospondins and craniofacial dysmorphism in mice overexpressing Fral2006

    • Author(s)
      Nishiwaki T.
    • Journal Title

      J.Bone Miner.Res. 21・4(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] c-Fos suppresses systemic inflammatory response to endotoxin2006

    • Author(s)
      Ray N.
    • Journal Title

      Int.Immunol. 17・(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Resorption of auditory ossicles and hearing loss in mice lacking osteoprotegerin.2006

    • Author(s)
      Kanzaki S.
    • Journal Title

      Bone 38・(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 破骨細胞分化にかかわる転写因子2005

    • Author(s)
      松尾光一
    • Journal Title

      日本臨牀 63・9

      Pages: 1541-1546

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 骨代謝制御因子としてのFosファミリー2005

    • Author(s)
      松尾光一
    • Journal Title

      The Bone 19・6

      Pages: 651-655

    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 炎症性疾患の検出並びに炎症性疾患の予防又は治療組成物2006

    • Inventor(s)
      丸山健太, 松尾光一, 保田尚孝
    • Industrial Property Rights Holder
      慶應義塾, オリエンタル酵母
    • Filing Date
      2006-05-12
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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