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Genetic variation in pain sensation and sensitivity to opioid

Research Project

Project/Area Number 17390431
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionShinshu University (2007)
Sapporo Medical University (2005-2006)

Principal Investigator

KAWAMATA Mikito  Shinshu University, School of Medicine, Professor (90315523)

Co-Investigator(Kenkyū-buntansha) KANAYA Noriaki  Sapporo Med. Univ, School of Med, Associate Professor (10244344)
YAMAUCHI Masahori  Sapporo Med. Univ, School of Med, Assistant Professor (00404723)
NARIMATSU Eichi  Sapporo Med. Univ, School of Med, Associate Professor (70295343)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥15,590,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥690,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥9,100,000 (Direct Cost: ¥9,100,000)
KeywordsSpinal cord dorsal horn / Substantia gelatinosa / Rostral Ventromedial Medulla / Pain sensitivity / Mouse / Patch-clamp / 延髄腹内側部 / 手術侵襲 / 系統 / 細胞外記録 / マウス系統 / μレセプタ
Research Abstract

Whole-cell patch clamp recordings were made from substantia gelatinosa neurons in mice under anesthesia with urethane. Both non-noxious air puff stimuli and noxious pinch stimuli with forceps were applied to the receptive field of each neuron on the hindpaw. A 5-mm-long incision was made through the skin, fascia and muscle of the center of the field. The skin was apposed with a 6-0 nylon suture. As a behavioral study, the highest score of spontaneous pain-related behavior was seen in CBA strain mice after incision, and the lowest score of the behavior was seen in A strain mice after incision. The spontaneous pain scores did not significantly increase in A strain mice after incision.
Rostral ventromedial medulla, RVM, neurons to mu agonist DAMGO was evaluated. As a behavioral study, an intracerebroventricular catheter was chronically implanted. The withdrawal latency to pinch stimuli was measured before and after intracerebroventricular injection of the mu receptor agonist DAMGO. A strai … More n mice were more sensitive to DAMGO than were CBA strain mice. In electrophysiological experiments, a tungsten microelectrode was advanced from the surface of the cerebellum into the ventral medulla to a depth of 400-500 〓m until action potentials from a single RVM neuron could be extracellularly isolated. An RVM neuron was classified as an ON, OFF or NEUTRAL cell by its responses to application of noxious stimuli. Spontaneous activity and evoked responses of the neurons to mechanical stimuli (pinch) of ON and OFF cells were recorded before and after intracerebroventricular injection of DAMGO. ON cells in A strain mice were more easily suppressed by application of DAMGO than in CBA strain mice. DAMGO more greatly suppressed the decrease in firing rates by pinch stimuli in A strain mice than were those in CBA strain mice. Thus, both ON and OFF cells in A strain mice were more sensitive to activation of mu receptors than were those in CBA strain mice.
Thus, response properties of neurons and networks of circuits in regions that are involved in pain processing are genetically determined. The difference in the response properties may be one of the mechanisms of variation in pain sensitivity and sensitivity to analgesics in humans. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (39 results)

All 2007 2006 2005

All Journal Article (29 results) (of which Peer Reviewed: 9 results) Presentation (9 results) Book (1 results)

  • [Journal Article] Lack in effects of therapeutic concentrations of dexmedetomidine and clonidine on the neuromuscular blocking action of rocuronium in isolated ratdiaphragms.2007

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, et. al.
    • Journal Title

      Anesth Analg 104

      Pages: 1116-1120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lack in effects of therapeutic concentrations of dexmedetomidine and clonidine on the neuromuscular blocking action of rocuronium in isolated rat diaphragms2007

    • Author(s)
      Narimatsu, E., Niiya, T., Kawamata, M., et. al.
    • Journal Title

      Anesth Analg 104

      Pages: 1116-20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lack in effects of therapeutic concentrations of dexmedetomidine and clonidine on the neuromuscular blocking action of rocuronium in isolated rat diaphragms2007

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, Namiki A.
    • Journal Title

      Anesth Analg 104

      Pages: 1116-20

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 手術侵襲による痛みのメカニズム2007

    • Author(s)
      川真田樹人
    • Journal Title

      麻酔 56

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] カンナビノイドによる鎮痛の機序と治療への応用2007

    • Author(s)
      川真田樹人, 木谷友洋
    • Journal Title

      医学のあゆみ 223

      Pages: 760-764

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in properties of substantia gelatinosa neurons following surgical incision in the rat: in vivo patch-clamp analysis.2006

    • Author(s)
      Kawamata M, Furue H, Kozuka Y, et. al.
    • Journal Title

      Anesthesiology 104

      Pages: 432-440

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of systemic administration of lidocaine and QX-314 onhyperexcitability of spinal dorsal horn neurons after incision in the rat.2006

    • Author(s)
      Kawamata M, Sugino K, Narimatsu, et. al.
    • Journal Title

      Pain 122

      Pages: 68-80

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Non-invasive positive pressure ventilation for laryngeal contraction disorderduring sleep in multiple system atrophy.2006

    • Author(s)
      Nonaka M, Imai T, Shintani T, Kawamata M, et. al.
    • Journal Title

      J Neurol Sciences 247

      Pages: 53-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Changes in properties of substantia gelatinosa neurons following surgical incision in the rat : in vivo patch-clamp analysis2006

    • Author(s)
      Kawamata, M., Furue, H., Kozuka, Y., et. al.
    • Journal Title

      Anesthesiology 104

      Pages: 432-440

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of systemic administration of lidocaine and QX-314 on hyperexcitability of spinal dorsal horn neurons after incision in the rat2006

    • Author(s)
      Kawamata, M., Sugino, K., Narimatsu, et. al.
    • Journal Title

      122

      Pages: 68-80

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Non-invasive positive pressure ventilation for laryngeal contraction disorder during sleep in multiple system atrophy2006

    • Author(s)
      Nonaka, M., Imai, T., Shintani, T., Kawamata, M., et. al.
    • Journal Title

      J Neurol Sciences 247

      Pages: 53-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in properties of substantial gelatinosa neurons after surgical incision in the rat : in vivo patch-clamp analysis2006

    • Author(s)
      Kawamata M, Furue H, Kotuku Y, Narimatsu E, Yoshimura M, Namiki A.
    • Journal Title

      Anesthesiology 104

      Pages: 432-40

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of systemic administration of lidocaine and QX-314 on hyperexcitability of spinal dorsal horn neurons after incision in the rat.2006

    • Author(s)
      Kawamata M, Sugino S, Narimatsu E, Yamauchi M, Kiya T, Furuse S, Namiki A.
    • Journal Title

      Pain 122

      Pages: 68-80

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recent advances in basic neurosciences and brain disease : from synapses to behavior.2006

    • Author(s)
      Bi GQ., Bolshakov V., Bu G., Cahill CM., Chen ZF., Collingridge GL., Cooper RL., Coorssen JR., El-Husseini A., Galhardo V., Gan WB., Gu J., Inoue K., Isaac J., Iwata K., Jia Z., Kaang BK., Kawamata M., Kida S., Klann E., Kohno T., Li M., Li XJ., MacDonald JF., Nader K., Kguyen PV., Oh U., Ren K., Roder JC., Salter MW., Song W., Sugita S., Tang SJ., Tao Y., Wang YT., Woo N., Woodin MA., Yan Z., Yoshimura M., Xu M., Xu ZC., Zhang X., Zhen M., Zhuo M.
    • Journal Title

      Mol. Pain 2

      Pages: 38-59

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Non-invasive positive pressure ventilation for laryngeal contraction2006

    • Author(s)
      Konaka M., Imai T, Shintani T, Kawamata M, Chiba S., Matsumoto H.
    • Journal Title

      J Neurol Sci 247

      Pages: 53-58

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 【麻酔科医療の現状と課題】ペインクリニックの現況2006

    • Author(s)
      並木昭義, 川真田樹人
    • Journal Title

      日本医師会雑誌 135

      Pages: 798-802

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 慢性疼痛に対するオピオイドの使用経験と問題点2006

    • Author(s)
      川真田樹人, 並木昭義
    • Journal Title

      ペインクリニック 28

      Pages: 347-354

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of halothane and isoflurane on hyperexcitability of spinal dorsal horn neurons after incision in the rat.2005

    • Author(s)
      Kawamata M, Narimatsu E, Kozuka Y, et. al.
    • Journal Title

      Anesthesiology 102

      Pages: 165-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Changes in properties of spinal dorsal horn neurons and their sensitivityto morphine after spinal cord injury in the rat.2005

    • Author(s)
      Wang J, Kawamata M, Namiki A
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Changes in response properties and receptive fields of spinal dorsal hornneurons in rats after surgical incision in hairy skin.2005

    • Author(s)
      Kawamata M, Koshizaki M, Narimatsu E, et. al.
    • Journal Title

      Anesthesiology 102

      Pages: 141-151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of halothane and isoflurane on hyperexcitability of spinal dorsal horn neurons after incision in the rat2005

    • Author(s)
      Kawamata, M., Narimatsu, E., Kozuka, Y., et. al.
    • Journal Title

      Anesthesiology 102

      Pages: 165-174

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Namiki A Changes in properties of spinal dorsal horn neurons and their sensitivity to morphine after spinal cord injury in the rat2005

    • Author(s)
      Wang, J., Kawamata, M
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Changes in response properties and receptive fields of spinal dorsal horn neurons in rats after surgical incision in hairy skin2005

    • Author(s)
      Kawamata, M., Koshizaki, M., Narimatsu, E., et. al.
    • Journal Title

      Anesthesiology 102

      Pages: 141-151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of halothane and isoflurane on hyperexcitability of spinal dorsal horn neurons after incision in the rat.2005

    • Author(s)
      Kawamata M, Narimatsu E, Kozuka Y, et al.
    • Journal Title

      Anesthesiology 102

      Pages: 165-174

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Changes in properties of spinal dorsal horn neurons and their sensitivity to morphine after spinal cord injury in the rat.2005

    • Author(s)
      Wang J, Kawamata M, Namiki A
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Changes in response properties and receptive fields of spinal dorsal horn neurons in rats after surgical incision in hairy skin.2005

    • Author(s)
      Kawamata M, Koshizaki M, Narimatsu E et al.
    • Journal Title

      Anesthesiology 102

      Pages: 141-151

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sepsis stage dependently and differentially attenuates the effects of nondepolarizing neuromuscular blockers on the rat diaphragm in vitro.2005

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, Namiki A
    • Journal Title

      Anesthesia and Analgsia 100

      Pages: 823-829

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 帝王切開-全身麻酔2005

    • Author(s)
      川真田樹人
    • Journal Title

      産婦人科の世界 57

      Pages: 299-305

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 骨盤痛のメカニズム2005

    • Author(s)
      川真田樹人, 並木昭義
    • Journal Title

      産婦人科の世界 57

      Pages: 299-305

    • Related Report
      2005 Annual Research Report
  • [Presentation] Changes in synaptic transmission in substantia gelatinosa neurons afterapplication of mustard oil.2007

    • Author(s)
      Kawamata M
    • Organizer
      Neuroscience
    • Place of Presentation
      San Diego
    • Year and Date
      2007-11-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nitrous oxide does not affec amplitude of motor-evoked potentials.2006

    • Author(s)
      Kawamata M, Narimatsu E, Yamauchi M, et. al.
    • Organizer
      アメリカ麻酔学会総会
    • Place of Presentation
      シカゴ、アメリカ
    • Year and Date
      2006-10-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Properties of substantia gelatinosa neruons in neonatal rats by in vivopatch-clamp analysis.2006

    • Author(s)
      Kawamata M, Sugino S, Narimatsu E, et. al.
    • Organizer
      アメリカ麻酔学会総会
    • Place of Presentation
      シカゴ、アメリカ
    • Year and Date
      2006-10-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Nitrous Oxide does not affect amplitude of motor-evoked potentials2006

    • Author(s)
      Kawamata, M., Narimatsu, E., Yamauchi, M., et. al.
    • Organizer
      Annual Meeting of the American Society of Anesthesiologists
    • Place of Presentation
      Chicago
    • Year and Date
      2006-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Properties of substantia gelatinosa neurons in neonatal rats by in vivo patch-clamp analysis2006

    • Author(s)
      Kawamata, M., Sugino, S., Narimatsu, E., et. al.
    • Organizer
      Annual Meeting of the American Society of Anesthesiologists
    • Place of Presentation
      Chicago
    • Year and Date
      2006-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differences in action potentials of spinal dorsal horn neurons after spinal cord ischemia in the rat2006

    • Author(s)
      Sugino S, Kawamata M, et. al.
    • Organizer
      アメリカ麻酔学会総会
    • Place of Presentation
      シカゴ、アメリカ
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differences in action potentials of spinal dorsal horn neurons after spinal cord ischemia in the rat2006

    • Author(s)
      Sugino, S., Kawamata, M., Yamauchi, M., et. al.
    • Organizer
      Annual Meeting of the American Society of Anesthesiologists
    • Place of Presentation
      Chicago
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic variation in response of spinal dorsal horn neurons and rostral ventromedial medulla neurons in different mouse strains.2006

    • Author(s)
      Kawamata M
    • Organizer
      シナプス記憶薬物依存国際カンファレンス
    • Place of Presentation
      トロント,カナダ
    • Year and Date
      2006-08-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic variation in response properties of spinal dorsal horn neurons and rostral ventromedial medulla neurons in different mouse strain2006

    • Author(s)
      Kawamata, M
    • Organizer
      International Conference-Synapses-Memory-Drug addiction-Pain
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2006-08-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 分娩痛とオピオイド.オピオイド(並木昭義, 表圭一編集)2005

    • Author(s)
      川真田樹人, 高橋稔之
    • Total Pages
      8
    • Publisher
      克誠堂出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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