Novel therapy for muscle invasive bladder cancer using glyco-biological approach

• Project/Area Number: 17390434
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Tohoku University
• Principal Investigator: SATOH Makoto Tohoku University, 大学院・医学系研究科, 非常勤講師 (70282134)
• Co-Investigator(Kenkyū-buntansha): SAITO Seiichi 琉球大学, 医学部, 教授 (80235043) ; ITO Akihiro 東北大学, 大学病院, 講師 (70344661) ; NUMAHATA Kenji 東北大学, 大学院・医学系研究科, 助手 (60359502)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,280,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2006: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
• Keywords: 浸潤性膀胱癌 / ガングリオシドGM3 / 増殖抑制 / 運動能抑制 / GM3 / 膀胱癌細胞 / Ga1NAcβ1-4糖転移酵素 / interference RNA / EGF-R / マウス膀胱癌モデル / 膀胱内注入療法 / アシアロGM3 / マウス表在性膀胱癌モデル / spreading assay / Glycosynapse分子

Research Abstract

浸潤性膀胱癌に対する新しい治療法の開発を目的として、ガングリオシド糖鎖GM3の膀胱癌細胞に対する影響を解析し、膀胱癌細胞およびマウス膀胱癌モデルに対するGM3の抗腫瘍効果を検討した。その結果、ガングリオシドGM3添加による膀胱癌細胞の増殖抑制効果、運動能抑制効果が判明し、さらに、マウス膀胱癌モデルに対するGM3の抗腫瘍効果が認められた。このことよりGM3による膀胱癌治療の可能性が示唆された。

Research Products

• [Journal Article] Haptoglobin-beta chain defined by monoclonal antibody RM2 as a novel serum marker for prostate cancer (2008)
  • Author(s): Saito S, Murayama Y, Pan Y, Taima T, Fujimura T, Murayama K, Sadilek M, Egawa S, Ueno S, Ito A, Ishidoya S, Nakagawa H, Kato M, Satoh M, Endoh M, Arai Y.
  • Journal Title: Int J Cancer.Aug 1 123(3) Pages: 633-640
  • Peer Reviewed
• [Journal Article] The Outcome of Prostate Cancer Screening in a Normal Japanese Population with PSA of 2-4 ng/ml and the Free/Total PSA Under 12% (2008)
  • Author(s): Ishidoya S, Ito A, Orikasa K, Kawamura S, Tochigi T, Kuwahara M, Yamanobe T, Tomita Y, Masumori N, Tsukamoto T, Shibuya D, Arai Y.
  • Journal Title: Jpn J Clin Onco 138 Pages: 844-848
  • Peer Reviewed
• [Journal Article] Survival of metastatic germ cell cancer patients assessed by international germ cell consensus classification in Japan (2008)
  • Author(s): Shintaku I, Satoh M, Okajima E, Fujimoto H, Kamoto T, Ogawa O, Kawai K, Akaza H, Tsukamoto T, Naito S, Miki T, Arai Y.
  • Journal Title: Jpn J Clin Oncol. Apr 38(4) Pages: 281-287
  • Peer Reviewed
• [Journal Article] Spontaneous regression of pulmonary metastases from testicular embryonal carcinoma (2008)
  • Author(s): Masanori Kato, Yoshihiro Ikeda, Shunichi Namiki, Seiichi Saito, Akihiro Ito and Yoichi Arai
  • Journal Title: Int J Urol 15 Pages: 265-266
  • NAID: 10021277243
  • Peer Reviewed
• [Journal Article] Sexual Function Reported by Japanese and American Men (2008)
  • Author(s): Shunichi Namiki, Lorna Kwan, Marjorie Kagawa-Singer, Seiichi Saito, Akito Terai, Takefumi Satoh, Shiro Baba, Yoichi Arai and Mark S. Litwin
  • Journal Title: THE JOURNAL OF UROLOGY 179 Pages: 245-249
  • Peer Reviewed
• [Journal Article] Haptoglobin-beta chain defined by monoclonal antibody RM2 as a novel serum marker for prostate cancer. I (2008)
  • Author(s): Saito S, Ito A, M, Satoh M
  • Journal Title: Int J Cancer 23 Pages: 633-640
  • Peer Reviewed
• [Journal Article] The Outcome of Prostate Cancer Screening in a Normal Japanese Population with PSA of 2-4 ng / ml and the Free / Tctal PSA Under 12% (2008)
  • Author(s): Ishidoya S, Ito A
  • Journal Title: Jon J Clin Onco1 38 Pages: 844-848
  • Peer Reviewed
• [Journal Article] Oncolytic virotherapy for prostate cancer by E1A, E1B mutant adenovirus (2007)
  • Author(s): Satoh M, Wang H, Ishidoya S, Abe H, Moriya T, Hamada H, Arai Y
  • Journal Title: Urology 70(6) Pages: 1243-1248
  • Peer Reviewed
• [Journal Article] and the Tohoku-Kyoto Urinary Reconstruction Study Group (2007)
  • Author(s): Masanori Kato, Atsushi Takeda,Seiichi Saito, Akito Terai, Yoji Taki, Shinnosuke Kato, Yusaku Okada, Osamu Ogawa, Yoichi Arai
  • Journal Title: Long-Term Functional Outcomes of Ileal and Sigmoid Orthotopic Neobladder Procedures Urology69(1) Pages: 74-77
  • Peer Reviewed
• [Journal Article] Impact of Unilateral Sural Nerve Graft on Recovery of Potency and Continence Following Radical Prostatectomy (2007)
  • Author(s): Shunichi Namiki, Seiichi Saito, Haruo Nakagawa, Takehiko Sanada, Atsushi Yamada and Yoichi Arai
  • Journal Title: THE JOURNAL OF UROLOGY 178 Pages: 212-216
  • Peer Reviewed
• [Journal Article] Impact of salvage therapy for biochemical recurrence on health-related quality of life following radical prostatectomy (2007)
  • Author(s): Shunichi Namiki, Seiichi Saito,Tatsuo Tochigi, Naomasa Ioritani, Akito Terai and Yoichi Arai
  • Journal Title: Int J Urol 14 Pages: 186-191
  • Peer Reviewed
• [Journal Article] Impact of unilateral interposition sural nerve graft on the recovery of sexual function after radical prostatectomy in Japanese men : a preliminary study (2007)
  • Author(s): Saito S, Namiki S, Numahata K, Satoh M, Ishidoya S, Ito A, Nakagawa H, Kaiho Y, Sanada T, Yamada A, Arai Y.
  • Journal Title: Int J Urol.Feb 14(2) Pages: 133-139
  • NAID: 10018849947
  • Peer Reviewed
• [Journal Article] Oncolytic virotherapy for prostate cancer by E1A,E1B mutant adenovirus. (2007)
  • Author(s): Makoto Satoh
  • Journal Title: Urology 70(6) Pages: 1243-8
  • Peer Reviewed
• [Journal Article] Long-Term Functional Outcomes of Ileal and Sigmoid Qrthotopic Neobladder Procedures (2007)
  • Author(s): Masanori Kato, Seiichi Saito
  • Journal Title: Urology 69(1) Pages: 74-77
  • Peer Reviewed
• [Journal Article] Impact of Unilateral Sural Nerve Graft on Recovery of Potency and Continence Following Radical Prostatectomy (2007)
  • Author(s): Shunichi Namiki, Seiichi Saito
  • Journal Title: THE JOURNAL OF UROLOGY 178 Pages: 212-216
  • Peer Reviewed
• [Journal Article] Relevance of postcatheter removal incontinence to postoperative urinary function after radical prostatectomy (2006)
  • Author(s): Saito S, Namiki S, Numahata K, Satoh M, Ishidoya S, Ito A, Arai Y.
  • Journal Title: Int J Urol. Sep 13(9) Pages: 1191-1196
  • NAID: 10018303353
  • Peer Reviewed
• [Journal Article] Plasma membrane-associated sialidase is up-regulated in renal cell carcinoma and promotes interleukin-6-induced apoptosis suppression and cell motility (2006)
  • Author(s): Ueno S, Saito S, Wada T, Yamaguchi K, Satoh M, Arai Y, Miyagi T
  • Journal Title: J Biol Chem.Mar 24 281(12) Pages: 7756-7764
  • Peer Reviewed
• [Journal Article] Health-Related Quality of Life after Intensity Modulated Radiation Therapy for Localized Prostate Cancer : Comparison with Conventional and Conformal Radiotherapy (2006)
  • Author(s): Shunichi Namiki, Shigeto Ishidoya, Tatsuo Tochigi, Sadafumi Kawamura, Masaaki Kuwahara, Akito Terai, Koji Yoshimura, Isao Numata, Makoto Satoh, Seiichi Saito, Yoshihiro Takai, Shogo Yamada and Yoichi Arai
  • Journal Title: Jpn J Clin Oncol 36(4) Pages: 224-230
  • Peer Reviewed
• [Journal Article] Oncolytic viral therapy by bladder instillation using an E1A, E1B double-restricted adenovirus in an orthotopic bladder cancer model (2006)
  • Author(s): Wang H, Satoh M, Abe H, Sunamura M, Moriya T, Ishidoya S, Saito S, Hamada H, Arai Y.
  • Journal Title: UROLOGY Sep 68(3) Pages: 674-681
  • Peer Reviewed
• [Journal Article] NATURAL HISTORY OF VOIDING FUNCTION AFTER RADICAL RETROPUBIC PROSTATECTOMY (2006)
  • Author(s): SHUNICHI NAMIKI, SHIGETO ISHIDOYA, SEIICHI SAITO, MAKOTO SATOH, TATSUO TOCHIGI, NAOMASA IORITANI, KOJI YOSHIMURA, AKITO TERAI, AND YOICHI ARAI
  • Journal Title: UROLOGY 68(1) Pages: 142-147
  • Peer Reviewed
• [Journal Article] Gene therapies for bladder cancer (2005)
  • Author(s): Satoh M, Wang H, Arai Y.
  • Journal Title: Nippon Rinsho.Dec ; 63 Suppl 12 Pages: 548-551
  • Peer Reviewed
• [Presentation] モノクローナル抗体RM2により規定されるGPXの前立腺癌患者血清中における増加 (2007)
  • Author(s): 斎藤誠一、荒井陽一
  • Organizer: 第45回日本癌治療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2007-10-25
• [Presentation] RM2 antigen as a new marker for early prostate cancer (2007)
  • Author(s): Saito S, Murayama Y, Ueno S, Ito A.
  • Organizer: The First International Symposium on Prostate Cancer
  • Place of Presentation: ChangChun, China
  • Year and Date: 2007-07-21
• [Presentation] ワークショップ泌尿器科領域の再生医療の進歩 : 海綿体神経再建術の現状と問題点 (2007)
  • Author(s): 斎藤誠一、沼畑健司、伊藤明宏
  • Organizer: 第95回日本泌尿器科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2007-04-15
• [Presentation] 総会特別企画1小切開手術 : 内視鏡下小切開前立腺全摘除術 (2007)
  • Author(s): 伊藤明宏、沼畑健司、斎藤誠一、荒井陽一
  • Organizer: 第95回日本泌尿器科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2007-04-14
• [Presentation] RM2 antigen as a new marker for prostate cancer (2007)
  • Author(s): Saito S, Egawa S, Hakomori S, Arai Y.
  • Organizer: Glycobiology and Sphingobiology 2007. Hakomori Commemorative Forum Tokushima
  • Place of Presentation: Japan
  • Year and Date: 2007-02-28
• [Presentation] 再燃前立腺癌に対する化学療法 (2007)
  • Author(s): 沼畑健司
  • Organizer: 第95回日本泌尿器科学会総会
  • Place of Presentation: 神戸
• [Presentation] 精巣腫瘍における後腹膜鏡視下後腹膜リンパ節郭清術 (2006)
  • Author(s): 伊藤明宏、佐藤信、荒井陽一
  • Organizer: 第19回日本内視鏡外科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2006-12-07
• [Presentation] 精巣癌・腎盂尿管癌の後腹膜リンパ節郭清術の工夫精巣腫瘍に対する後腹膜鏡視下後腹膜リンパ節郭清術 (2006)
  • Author(s): 伊藤明宏、佐藤信、斎藤誠一、荒井陽一
  • Organizer: 第20回日本E & E学会
  • Place of Presentation: 大阪
  • Year and Date: 2006-10-06
• [Presentation] シンポジウムII糖鎖研究が明らかにする疾患発症機構とその応用 : 前立腺癌の糖鎖マーカーRM2抗原(b 1,4-GalNAc-disialyl-Lc4) (2006)
  • Author(s): 斎藤誠一、頴川晋、伊藤明宏
  • Organizer: 第26回日本糖質学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2006-08-25
• [Presentation] External renal hypothermia using ice-cold saline for retroperitoneoscopic partial nephrectomy (2006)
  • Author(s): Ito A, Satoh M, Numahata K, Saito S.
  • Organizer: 24th World congress of Endourology. Cleveland, Ohaio
  • Place of Presentation: USA
  • Year and Date: 2006-08-19
• [Presentation] Extra-peritoneal laparoscopic radical cystourethrectomy with bilateral nephrouretectomy for the patient withmladder cancer and the maintenance hemodialysis (2006)
  • Author(s): Numahata K,Ito A, Satoh M, Saito S.
  • Organizer: 24th World congress of Endourology. Cleveland, Ohaio
  • Place of Presentation: USA
  • Year and Date: 2006-08-18
• [Presentation] シンポジウム「前立腺癌ノモグラムと予後因子としてのバイオマーカー」、前立腺癌の糖鎖マーカーRM2抗原(b 1,4-GalNAc- disialyl -Lc 4) : 組織および血清における発現 (2006)
  • Author(s): 斎藤誠一、頴川晋、伊藤明宏、荒井陽一
  • Organizer: 第33回尿路悪性腫瘍研究会
  • Place of Presentation: 東京
  • Year and Date: 2006-07-22
• [Presentation] 氷冷生食水を用いた局所冷却による腹腔鏡下腎部分切除術 (2006)
  • Author(s): 伊藤明宏、新宅一郎、佐藤信、荒井陽一
  • Organizer: 第88回泌尿器科談話会, 第8回東北泌尿器科EBMフォーラム
  • Place of Presentation: 仙台
  • Year and Date: 2006-06-17
• [Presentation] RM2 antigen(beta1,4-GalNAc-disialyl-Lc4) as a new serum marker for prostate cancer (2006)
  • Author(s): S Saito,S Ueno, A Ito, K Numahata
  • Organizer: AUA 101th Annual Meeting
  • Place of Presentation: Atlanta
  • Year and Date: 2006-03-22
• [Presentation] 制限増殖型アデノウイルスAxdAdB-3と塩酸Gemcitabineを併用した腎癌に対する治療効果の検討 (2006)
  • Author(s): 王華、佐藤信、伊藤明宏、斎藤誠一
  • Organizer: 第65回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Remarks] 東北大学大学院医学系研究科泌尿器科学教室
  • URL: http://www.uro.med.tohoku.ac.jp/
• [Remarks]
  • URL: http://www.uro.med.tohoku.ac.jp/