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Basic studies of three dimensionally stacked retinal prosthesis toward for clinical trials

Research Project

Project/Area Number 17390465
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTohoku University

Principal Investigator

TOMITA Hiroshi  Tohoku University, Tohoku University, Biomedical Engineering Research Organization, Associate professor (40302088)

Co-Investigator(Kenkyū-buntansha) MAKOTO Tamai  Tohoku University, Professor (90004720)
SUGANO Eriko  Tohoku University, Research Associate (70375210)
ISAGO Hitomi  Tohoku University, Technical Assistant (30400451)
小柳 光正  東北大学, 大学院工学研究科, 教授 (60205531)
佐藤 まなみ  東北大学, 先進医工学研究機構, 技術補佐員 (80375211)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,780,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2005: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsretinal prosthesis / retinal degeneration / three dimensional stacked technology / rabbits
Research Abstract

We have already decided appropriate material for stimulus electrode and parameters such as current and patterns to stimulate a retina. We reported the differences of behavioral activities in between normal rabbits and photoreceptor degenerated rabbits with experiments using a maze. The purpose in this year is to clarify the visual acuity in animals which was implanted our developed retinal prosthesis. For this purpose, we chose cynomorgus monkeys as experimental animals because we can apply the same method as evaluating the visual acuity for human. However there is no report to degenerate photoreceptors in monkeys. To degenerate photoreceptors in a monkey, we tested three chemical compounds, sodium iodate, N-methyl-nitrosourea (NMU), and sodium nitropruside (SNP) which have been reported to degenerate photoreceptors in rabbits and rats. When we injected sodium iodate intravenously or NMU intraperitonealy amplitude of visually evoked potential was decreased. But we could not induce the degeneration of photoreceptors completely. By using rabbits, we tried to investigate effects of stimulus current on the visual acuity. We implanted electrode array which has various size of each electrode onto a retina, and recorded evoked potentials from 64 points in primary visual cortex. The responses induced by high current stimulation as 500 uA were observed in a wide area in the primary visual cortex. These results may indicate that lower current is suitable for giving higher resolution.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (60 results)

All 2008 2007 2006 2005 Other

All Journal Article (32 results) (of which Peer Reviewed: 14 results) Presentation (24 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Brain-derived neurotrophic factor increases the phagocytic activity mediated through mitogen-activated protein kinase pathwayin cultured iris pigment epithelial cells2008

    • Author(s)
      Yoshida , et. al.
    • Journal Title

      Cell structure and Function (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Brain-derived neurotrophic factor increases the phagocytic activity mediated through mitogen-activated protein kinase pathway in cultured iris pigment epithelial cells2008

    • Author(s)
      Yoshida et. al.
    • Journal Title

      Cell structure and Function (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Retinal ganglion cell protection by 17-beta-estradiol in a mouse model of inherited glaucoma2008

    • Author(s)
      Zhou X et. al.
    • Journal Title

      Dev Neurobiol. 67

      Pages: 603-616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Brain-derived neurotrophic factor increases the phagocytic activity mediated through mitogen-activated protein kinase pathway in cultured iris pigment epithelial cells2008

    • Author(s)
      Yoshida, et. al.
    • Journal Title

      Cell structure and Function (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinal ganglion cell protection by 17-beta-estradiol in a mouse model of inherited glaucoma2007

    • Author(s)
      Zhou X , et. al.
    • Journal Title

      Dev Neurobiol. 67

      Pages: 603-616

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characteristics of Mitochondrial Calpains.2007

    • Author(s)
      Ozaki T. , et. al.
    • Journal Title

      J Biochem. 142

      Pages: 365-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Restoration of Visual responses in aged dystrophic RCS rats by AAV-mediated channelopsin-2 gene transfer.2007

    • Author(s)
      Tomita H. , et. al.
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 3821-3826

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Hypothermia protects cultured human retinal pigment epithelial cells against indocyanine green toxicity.2007

    • Author(s)
      Kunikata H. , et. al.
    • Journal Title

      J Ocul Pharmacol Ther. 23

      Pages: 35-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Evaluation of Platinum-Black (Pt-b) Stimulus Electrode Array for Electrical Stimulation to Retinal Cells in Retinal Prosthesis System.2007

    • Author(s)
      Watanabe T. , et. al.
    • Journal Title

      Japanese Journal of Applied Physics 46

      Pages: 2785-2791

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nonredundant role of Akt2 for neuroprotection of rod photoreceptor cells from light-induced cell death.2007

    • Author(s)
      Li G. , et. al.
    • Journal Title

      J. Neurosci. 27

      Pages: 203-211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characteristics of Mitochondrial Calpains2007

    • Author(s)
      Ozaki T. et. al.
    • Journal Title

      J Biochem. 142

      Pages: 365-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Restoration of Visual responses in aged dystrophic RCS rats by AAV-mediated channelopsin-2 gene transfer2007

    • Author(s)
      Tomita H. et. al.
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 3821-3826

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Evaluation of Platinum-Black (Pt-b) Stimulus Electrode Array for Electrical Stimulation to Retinal Cells in Retinal Prosthesis System2007

    • Author(s)
      Watanabe T. et. al.
    • Journal Title

      Japanese Journal of Applied Physics 46

      Pages: 2785-2791

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nonredundant role of Akt2 for neuroprotection of rod photoreceptor cells from light-induced cell death2007

    • Author(s)
      Li G. et. al.
    • Journal Title

      J. Neurosci. 27

      Pages: 203-211

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Retinal ganglion cell protection by 17-beta-estradiol in a mouse model of inherited glaucoma2007

    • Author(s)
      Zhou X, et. al.
    • Journal Title

      Dev Neurobiol. 67

      Pages: 603-616

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of Mitochondrial Calpains.2007

    • Author(s)
      Ozaki T., et. al.
    • Journal Title

      J Biochem. 142

      Pages: 365-376

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Restoration of Visual responses in aged dystrophic RCS rats by AAV-mediated channelopsin-2 gene transfer.2007

    • Author(s)
      Tomita H., et. al.
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 3821-3826

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypothermia protects cultured human retinal pigment epithelial cells against indocyanine green toxicity.2007

    • Author(s)
      Kunikata H., et. al.
    • Journal Title

      J Ocul Pharmacol Ther. 23

      Pages: 35-39

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of Platinum-Black(Pt-b)Stimulus Electrode Array for Electrical Stimulation to Retinal Cells in Retinal Prosthesis System.2007

    • Author(s)
      Watanabe T., et. al.
    • Journal Title

      Japanese Journal of Applied Physics 46

      Pages: 2785-2791

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nonredundant role of Akt2 for neuroprotection of rod photoreceptor cells from light-induced cell death.2007

    • Author(s)
      Li G., et. al.
    • Journal Title

      J. Neurosci. 27

      Pages: 203-211

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visual responses in aged dystrophic RCS rats were restored by AAV-mediated channelopsin-2 gene transfer.2007

    • Author(s)
      Tomita H., et al.
    • Journal Title

      Invest. Ophthalmol. Vis. Sci. (In Press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nonredundant role of Akt2 for neuroprotection of rodphotoreceptor cells from light-induced cell death.2007

    • Author(s)
      Li.G., et al.
    • Journal Title

      J. Neurosci. 27(1)

      Pages: 203-211

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hypothermia protects cultured human retinal pigment epithelial cells against trypan blue toxicity.2006

    • Author(s)
      Kunikata H., et al.
    • Journal Title

      Ophthalmologica 220(2)

      Pages: 114-117

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of indocyanine green toxicity to rat retinas.2006

    • Author(s)
      Sato, Y., et al.
    • Journal Title

      Ophthalmologica 220(3)

      Pages: 153-158

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitric oxide-induced accumulation of lipofuscin-like materials is caused by inhibition of cathepsin S.2006

    • Author(s)
      Sugano, E., et al.
    • Journal Title

      Curr Eye Res 31(7-8)

      Pages: 607-616

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recombinant AAV-transduced iris pigment epithelial cell transplantation may transfer vector to native RPE but suppress systemic dissemination.2006

    • Author(s)
      Yoshioka, Y., et al.
    • Journal Title

      Invest Ophthalmol Vis Sci 47(2)

      Pages: 745-752

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluation of indocyanine green toxicity on ra retinas.2006

    • Author(s)
      Sato, Y.
    • Journal Title

      Ophthalmologica (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Establishment of Effective Methods for Transducing Ggenes into Iris Pigment Epithelial Cells using Adeno-associated Virus, Type 2.2005

    • Author(s)
      Sugano E.
    • Journal Title

      Invest Ophthalmol Vis Sci. 46(9)

      Pages: 3341-3348

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of inflammation, degradation and apoptosis in a mouse model of glaucoma.2005

    • Author(s)
      Zhou X.
    • Journal Title

      J Biol Chem. 280(35)

      Pages: 31240-31248

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mechanism of protection from light-induced retinal degeneration by the synthetic antitoxidant phenyl-N-tert-butylnitrone.2005

    • Author(s)
      Tomita H.
    • Journal Title

      Invest Opthalmol Vis Sci. 46(2)

      Pages: 427-434

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Deleted in polyposis 1-like 1 gene (Dp1l1): a novel gene richly expressed in retinal ganglion cells.2005

    • Author(s)
      Sato H.
    • Journal Title

      Invest Ophthalmol Vis Sci. 46(3)

      Pages: 791-796

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hypothermia protects cultured human retinal pigment epithelial cells against indocyanine green toxicity

    • Author(s)
      Kunikata H. et. al.
    • Journal Title

      J Ocul Pharmacol Ther. 23

      Pages: 35-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 眼の機能にかかわる色素細胞の機能進化2007

    • Author(s)
      矢野 由希子, 他
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2007-08-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] 視機能回復への展望<光感受性遺伝子導入による視覚誘発電位の回復>2007

    • Author(s)
      富田浩史, 他
    • Organizer
      国際バイオEXPO
    • Place of Presentation
      東京ビックサイト
    • Year and Date
      2007-06-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Perspection for restoring vision2007

    • Author(s)
      Tomita H. ed. al.
    • Organizer
      International BioEXPO
    • Place of Presentation
      Tokyo Bigsite
    • Year and Date
      2007-06-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 眼球埋込み用人工網膜のための刺激電流の評価Evaluation of electrical stimulus current applied to retinal cells for implantable retinal prosthesis2007

    • Author(s)
      渡部泰一郎, 他
    • Organizer
      JAPAN NANO 2007
    • Place of Presentation
      東京大学
    • Year and Date
      2007-02-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Evaluation of electrical stimulus current applied to retinal cells for implantable retinal prosthesis2007

    • Author(s)
      Watanabe T, ed. al.
    • Organizer
      JAPAN NANO 2007
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-02-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光感受性遺伝子導入によるRCSラットの視覚誘発電位の回復2007

    • Author(s)
      富田浩史, 他
    • Organizer
      日本眼科学会
    • Place of Presentation
      大阪国際会議場
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 人工網膜に替わる新規視機能再建法の検討2007

    • Author(s)
      菅野江里子, 他
    • Organizer
      日本眼科学会
    • Place of Presentation
      大阪国際会議場
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 光障害による視細胞変性と光感受性遺伝子による光反応の回復2007

    • Author(s)
      菅野江里子, 他
    • Organizer
      日本眼科学会
    • Place of Presentation
      大阪国際会議場
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Behavioural response in the royal college of surgeons rat was improved by the channelopsin-2 gene transfer2007

    • Author(s)
      Tomita H., et. al.
    • Organizer
      Vision Research Conference, Retinal Degeneration and Gene Therapy (VRES)(網膜変性と遺伝子治療に関する視覚研究会議)
    • Place of Presentation
      ブロワードカントリーコンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differences of Visually Evoked Potentials Between in Young and Aged RCS Rats Transferred Channelopsin 2 Gene2007

    • Author(s)
      Tomita H., et. al.
    • Organizer
      ARVO(国際眼科研究会議)
    • Place of Presentation
      ブロワードカントリーコンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Stable Expression of Thioredoxins in RPE Cells Increases Cell Viabilities During Oxidative Stress2007

    • Author(s)
      Sugano E., et. al.
    • Organizer
      ARVO(国際眼科研究会議)
    • Place of Presentation
      ブロワードカントリーコンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Improvement of behavioural response in genetically blind rat transffered the channelopsin-2 gene.2007

    • Author(s)
      Tomita H., et. al.
    • Organizer
      Society for Neuroscience(北米神経科学会)
    • Place of Presentation
      サンディエゴコンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Restoration of visual responses in genetically blind rats using AAV-mediated channelopsin-2 gene transfer.2007

    • Author(s)
      Sugano E., et. al.
    • Organizer
      Society for Neuroscience(北米神経科学会)
    • Place of Presentation
      サンディエゴコンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Recovery of visually evoked potentials in the royal college of surgeons rat was improved by the channelopsin-2 gene transfer2007

    • Author(s)
      Tomita H., ed. al.
    • Organizer
      Congress of Japanese Ophthalmology
    • Place of Presentation
      Osaka Convention Center
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] New technique for restoring vision substituting for retinal prosthesis2007

    • Author(s)
      Sugano ed. al.
    • Organizer
      Congress of Japanese Ophthalmology
    • Place of Presentation
      Osaka Convention Center
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Recovery of light responses in photoreceptor degenerated rats by light sensitive gene transfer2007

    • Author(s)
      Sugano ed. al.
    • Organizer
      Congress of Japanese Ophthalmology
    • Place of Presentation
      Osaka Convention Center
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Behavioural response in the royal college of surgeons rat was improved by the channelopsin-2 gene transfer2007

    • Author(s)
      Tomita H., ed. al.
    • Organizer
      Vision Research Conference, Retinal Degeneration and Gene Therapy(VRES)
    • Place of Presentation
      Ft. Lauderdale, FL
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differences of Visually Evoked Potentials Between in Young and Aged RCS Rats Transferred Channelopsin 2 Gene2007

    • Author(s)
      Tomita H., ed. al.
    • Organizer
      ARVO
    • Place of Presentation
      Ft. Lauderdale, FL
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Stable Expression of Thioredoxins in RPE Cells Increases Cell Viabilities During Oxidative Stress2007

    • Author(s)
      Sugano E., ed. al.
    • Organizer
      ARVO
    • Place of Presentation
      Ft. Lauderdale, FL
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improvement of behavioural response in genetically blind rat transffered the channelopsin-2 gene2007

    • Author(s)
      Tomita H, ed. al.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      SanDiego, CA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Restoration of visual responses in genetically blind rats using AAV-mediated channelopsin-2 gene transfer2007

    • Author(s)
      Sugano E., ed. al.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      SanDiego, CA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Behavioural response in the royal college of surgeons rat was improved by the channelopsin-2 gene transfer2007

    • Author(s)
      Tomita H., et. al.
    • Organizer
      Vision Research Conference, Retinal Degeneration and Gene Therapy(VRES)(網膜変性と遺伝子治療に関する視覚研究会議)
    • Place of Presentation
      ブロワードカントリーコンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] 遺伝子導入による新しい視機能再建法2007

    • Author(s)
      富田浩史, 他
    • Organizer
      日本動物学会
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report
  • [Presentation] チャネルロドプシンー2遺伝子導入による遺伝盲ラットの視機能の回復2007

    • Author(s)
      菅野江里子, 他
    • Organizer
      日本動物学会
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report
  • [Book] 3次元システムインパッケージと材料技術バイオエレクトロニクスへの応用2007

    • Author(s)
      小柳光正、田中徹、富田浩史
    • Total Pages
      20
    • Publisher
      (株)シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Book] 臨床眼科2005

    • Author(s)
      富田 浩史
    • Total Pages
      5
    • Publisher
      医学書院
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 遺伝子導入による視覚機能の再建方法2007

    • Inventor(s)
      富田浩史、玉井信、菅野江里子
    • Industrial Property Rights Holder
      富田浩史、玉井信、菅野江里子
    • Filing Date
      2007-03-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 遺伝子改変による光感受性増大の方法2007

    • Inventor(s)
      富田浩史、菅野江里子
    • Industrial Property Rights Holder
      富田浩史、菅野江里子
    • Filing Date
      2007-12-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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