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Developmental maxillofacial morphogenesis and relevant molecular regulatory mechanisms in mice

Research Project

Project/Area Number 17390489
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

AOBA Takaaki  The Nippon Dental University, School of Life Dentistry at Tokyo, Professor (30028807)

Co-Investigator(Kenkyū-buntansha) TAYA Yuji  The Nippon Dental University, School of Life Dentistry at Tokyo, Associate Professor (30197587)
SHIMAZU Yoshihito  The Nippon Dental University, School of Life Dentistry at Tokyo, Lecturer (10297947)
SATO Kaori  The Nippon Dental University, School of Life Dentistry at Tokyo, Lecturer (90287772)
KANRI Yoriaki  The Nippon Dental University, School of Life Dentistry at Tokyo, Assistant Professor (40366761)
YAGISHITA Hisao  The Nippon Dental University, School of Life Dentistry at Tokyo, Associate Professor (50256989)
添野 雄一  日本歯科大学, 歯学部, 助手 (70350139)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,850,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥6,500,000 (Direct Cost: ¥6,500,000)
KeywordsOdontology / Pathology / Development / Morphogenesis / Process-fusion / Enithelial-mesenchymal interaction / Cleft formation / 3D reconstruction / 3次元解析
Research Abstract

The present study aimed at elucidating the molecular regulation of mandibular process-fusion and palatogenesis in mouse development. ICR mouse embryos were used. Specimens for gene expression and histomorphology were obtained from the medial region of the pharyngeal and mandibular arches at E9.5-11.5 and palatal processes at E13.5-14.5. Multifaceted events and phenotypic features of mouse craniofacial morphogenesis were investigated histologically and immunohistochemically. Gene expression profiles were analyzed in combination of microarray and real-time PCR. We also explored site-discrete gene expression profiles by comparing the median and lateral portions of the mandibular processes. Mesenchymal cell populations in the specified medial region were collected from cryosections using laser capture microdissection. cDNA microarray analysis disclosed distinct spatial-temporal expression patterns of transcription factors (e.g., Pax, Runx, Snaih, a bundle of growth factors and receptors (e … More .g., Shh, FGFs, TGF β, BMR ET1,), Rho families, and cell adhesion molecules (N-cadherin, CD44). Of interest, a large number of the genes (i.e., Ptx1, Dlx, dHand, eHand, Msx1, Bapx1, Barx1, Fgf2, Fgf8, ET1), which are supposed to be involved in epithelio-mesenchymal interaction, were identified as candidates that were differentially expressed at the medial region of mandibular arches during the fusion process. Genes relevant to skeletal muscle differentiation were also validated to be up- or down-regulated in accordance with the completion to medial fusion of mandibular processes and subsequent tongue development. In parallel studies, we developed organ culture models to assimilate in vivo events of molecular and cellular dynamics involved in mandibular fusion and tongue morphogenesis. In the funded period, we verified the effects of SHH and FGF signals on mandibular process fusions and myogenic cell proliferation, apoptosis and differentiation. The results including 3D constructs of developing mouse craniofacial structures in motion are presented in Web site (http://www.ndu.ac.jp/~pathhome/) as well as in forms of publications and abstracts. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (56 results)

All 2007 2006 2005 Other

All Journal Article (31 results) (of which Peer Reviewed: 13 results) Presentation (19 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Molecular mechanisms governing early myogenesis of mouse tongue2007

    • Author(s)
      Taya, Y.
    • Journal Title

      J of Oral Biosci 49

      Pages: 211-215

    • NAID

      10027102867

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Epigenetic inactivation of E-cadherin by promoter hypermethylation in oral carrinoma cells2007

    • Author(s)
      Maeda, G.
    • Journal Title

      Odontology 95

      Pages: 24-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Introduction of web mediated database for dental students'self-learning of histopathology and evaluation of learners'motivation and achievements2007

    • Author(s)
      Aoba, T.
    • Journal Title

      Journal of Multimedia Aided Education Research 3

      Pages: 115-124

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of web-mediated database including motion constructs as teaching and self-learning materials in the Pathology course2007

    • Author(s)
      Sato, K.
    • Journal Title

      J.Educational Application of Information Technologies 10

      Pages: 6-10

    • NAID

      40015744850

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms governing early myogenesis of mouse tongue. [Recent studies of craniofacial muscles MINI-REVIEW2007

    • Author(s)
      Taya, Y
    • Journal Title

      J of Oral Biosci 49

      Pages: 211-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Epigenetic inactivation of E-cadherin by promoter hypermethylation in oral carcinoma cells.2007

    • Author(s)
      Maeda, G
    • Journal Title

      Odontology 95

      Pages: 24-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Introduction of web-mediated database for dental students' self-learning of histopathology and evaluation of learners' motivation and achievements2007

    • Author(s)
      Aoba, T
    • Journal Title

      Journal of Multimedia Aided Education Research 3

      Pages: 115-124

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of web-mediated database including motion constructs as teaching and self-learning materials in the Pathology course.2007

    • Author(s)
      Sato, K
    • Journal Title

      J. Educational Application of Information Technologies 10

      Pages: 6-10

    • NAID

      40015744850

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Molecular mechanisms governing early myogenesis of mouse tongue2007

    • Author(s)
      Taya, Y.
    • Journal Title

      J of Oral Biosci. 49

      Pages: 211-215

    • NAID

      10027102867

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic inactivation of E-cadherin by promoter hypermethylation in oral carcinoma cells2007

    • Author(s)
      Maeda, G.
    • Journal Title

      Odontology 95

      Pages: 24-29

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯学部病理診断学におけるWeb教材の活用と学習効果の検証2007

    • Author(s)
      青葉孝昭
    • Journal Title

      メディア教育研究 3

      Pages: 115-124

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 病理学教科における動画教材コンテンツの開発と自学自習向けのWeb配信2007

    • Author(s)
      佐藤かおり
    • Journal Title

      IT活用教育方法研究 10

      Pages: 6-10

    • NAID

      40015744850

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fate of medial edge epithehum in mouse palatogenesis in vitro:apoptosis,migration,and epithelial-mesenchymal transformation2006

    • Author(s)
      Soeno, Y.
    • Journal Title

      J Oral Biosci 48

      Pages: 286-296

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Solubility properties of carbonatoapatites having discrete stoichiometric compositions2006

    • Author(s)
      Kurata, S.
    • Journal Title

      J Oral Biosci 48

      Pages: 114-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fate of medial edge epithelium in mouse palatogenesis in vitro : apoptosis, migration, and epithelial-mesenchymal transformation.2006

    • Author(s)
      Soeno, Y
    • Journal Title

      J Oral Biosci 48

      Pages: 286-296

    • NAID

      10027102347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Solubility properties of carbonatoapatites having discrete stoichiometric compositions2006

    • Author(s)
      Kurata, S
    • Journal Title

      J Oral Biosci 48

      Pages: 114-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fate of medial edge epithelium in mouse palatogenesis in vitro : apoptosis, migration, and epithelial-mesenchymal transformation2006

    • Author(s)
      Soeno, Y.
    • Journal Title

      J. Oral Biosci. 48・1

      Pages: 286-296

    • NAID

      10027102347

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 学内LANによる視覚教材の配信と病理診断学での学習効果2006

    • Author(s)
      佐藤かおり
    • Journal Title

      大学教育と情報 15・2

      Pages: 29-31

    • NAID

      40015275012

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Solubility properties of carbonatoapatites having discrete stoichiometric compositions2006

    • Author(s)
      Kurata, S.
    • Journal Title

      J. Oral Biosci. 48・2

      Pages: 114-125

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 日本歯科大学病理学教科での3次元動画教材に関するアンケート調査と使用経験2006

    • Author(s)
      島津徳人
    • Journal Title

      日歯教誌 21・1

      Pages: 78-82

    • NAID

      10017419200

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Contribution of sialic acid-binding adhesion to pathogenesis of experimental endocarditis caused by Streptococcus gordonii DL12006

    • Author(s)
      Takahashi, Y.
    • Journal Title

      Infect. Immun. 74・1

      Pages: 740-743

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Formation and properties of bioapatites in mammals2005

    • Author(s)
      Aoba, T.
    • Journal Title

      Current Topics in Crystal Growth 7

      Pages: 31-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2005 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinoma implications for E-cadherin expression and tumor progression2005

    • Author(s)
      Maeda, G.
    • Journal Title

      Int J Oncol 27

      Pages: 1535-1541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Epithelial-mesenchymal transformation during palatogenesis and cleft alate development2005

    • Author(s)
      Soeno, Y.
    • Journal Title

      Dentistry in Japan 41

      Pages: 13-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Formation and properties of bioapatites in mammals2005

    • Author(s)
      Aoba, T
    • Journal Title

      Current Topics in Crystal Growth 7

      Pages: 31-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinoma : implications for E-cadherin expression and tumor progression2005

    • Author(s)
      Maeda, G
    • Journal Title

      Int J Oncol 27

      Pages: 1535-1541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Epithelial-mesenchymal transformation during palatogenesis and cleft palate development2005

    • Author(s)
      Soeno, Y
    • Journal Title

      Dentistry in Japan 41

      Pages: 13-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Epithelial-mesenchymal transformation during palatogenesis and cleft palate development2005

    • Author(s)
      Soeno, Y.
    • Journal Title

      Dentistry in Japan 41

      Pages: 13-18

    • Related Report
      2005 Annual Research Report
  • [Journal Article] マウス舌初期発生での筋系譜細胞の移住現象と表現型の推移2005

    • Author(s)
      藤武貴尚
    • Journal Title

      小児歯誌 41

      Pages: 408-416

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinoma : implications for E-cadherin expression and tumor progression2005

    • Author(s)
      Maeda, G.
    • Journal Title

      Int.J.Oncol. 27

      Pages: 1535-1541

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular mechanism for connective tissue destruction by dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis2005

    • Author(s)
      Kumagai, Y.
    • Journal Title

      Infect.Immunol. 73

      Pages: 2655-2664

    • Related Report
      2005 Annual Research Report
  • [Presentation] マウス舌初期発生における外側舌隆起の形成とFGFシグナル2007

    • Author(s)
      鳥居 大祐
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      北海道・札幌市
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] マウス下顎突起の正中癒合部での遺伝子発現プロファイル2007

    • Author(s)
      田谷 雄二
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      北海道・札幌市
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Studies on mandibular process-fusion and tongue development in a culture model of mouse branchial arches : Effects of FGF sugnalling2007

    • Author(s)
      Torii, D
    • Organizer
      49th Japanese Association for Oral Biology
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Microarray analysis of gene expression profile at the medial region of the first branchial arch2007

    • Author(s)
      Taya, Y
    • Organizer
      49th Japanese Association for Oral Biology
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス口唇口蓋裂自然発症と口蓋棚間葉織の増殖との関係についての分子生物学的検索2007

    • Author(s)
      佐々木康成
    • Organizer
      第45回小児歯科学会大会
    • Place of Presentation
      東京都・江戸川区
    • Year and Date
      2007-07-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Epithelio-myogenic cell interaction and molecular regulation in mouse tongue development2007

    • Author(s)
      Taya, Y.
    • Organizer
      First Pan American Congress in Developmental Biology
    • Place of Presentation
      Cancun,Mexico
    • Year and Date
      2007-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Epithelio-myogenic cell interaction and molecular regulation in mouse tongue development2007

    • Author(s)
      Taya, Y
    • Organizer
      First Pan American Congress in Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2007-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Epithelio-myogenic cell interaction and molecular regulation in mouse tongue development2007

    • Author(s)
      Taya, Y.
    • Organizer
      First Pan American Congressin Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2007-06-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] Microarray analysis of molecular networks governing the mandibular fusion of mouse embryos2007

    • Author(s)
      Fujita, K.
    • Organizer
      First Pan American Congress in Developmental Biology
    • Place of Presentation
      Cancun,Mexico
    • Year and Date
      2007-06-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Microarray analysis of molecular networks governing the mandibular fusion of mouse embryos2007

    • Author(s)
      Fujita, K
    • Organizer
      First Pan American Congress in Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2007-06-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Microarray analysis of molecular networks governing the mandibular fusion of mouse embryos2007

    • Author(s)
      Fujita, K.
    • Organizer
      First Pan American Congressin Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2007-06-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] Signaling networks via the epithelio-mesenchymal interactions during early development of mouse tongue2006

    • Author(s)
      Taya, Y.
    • Organizer
      Society for Developmental Biology 65th Annual Meeting
    • Place of Presentation
      Michigan,USA
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Signaling networks via the epithelio-mesenchymal interactions during early development of mouse tongue2006

    • Author(s)
      Taya, Y
    • Organizer
      Society for Developmental Biology 65th Annual Meeting
    • Place of Presentation
      Michigan, USA
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interaction between epithelium and myogenic progenitor cells is required for tongue morphogenesis2005

    • Author(s)
      Taya, Y.
    • Organizer
      Society for Development Biology 64th Annual Meeting
    • Place of Presentation
      San Francisco,USA
    • Year and Date
      2005-07-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interaction between epithelium and myogenic progenitor cells is required for tongue morphogenesis2005

    • Author(s)
      Taya, Y
    • Organizer
      Society for Developmental Biology 64th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2005-07-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Migration and phenotype-transition of myogenic cell lineage in development of mouse tongue2005

    • Author(s)
      Taya, Y.
    • Organizer
      The 58th Annual Meeting of Japan Society for Cell Biolo
    • Place of Presentation
      Omiya,Japan
    • Year and Date
      2005-06-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Migration and phenotype-transition of myogenic cell lineage in development of mouse tongue2005

    • Author(s)
      Taya, Y
    • Organizer
      The 58th Annual Meeting of Japan Society for Cell Biology
    • Place of Presentation
      Omiya, Japan
    • Year and Date
      2005-06-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 3D-morphometric snalysis of degenerative TMJ changes in Klotho mice2005

    • Author(s)
      Kanri, Y.
    • Organizer
      83rd General Session&Exhibition of the IADR
    • Place of Presentation
      Baltimore,USA
    • Year and Date
      2005-03-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 3D-morphometric snalysis of degenerative TMJ changes in Klotho mice2005

    • Author(s)
      Kanri, Y
    • Organizer
      83rd General Session & Exhibition of the IADR
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2005-03-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 誰にでもできる立体構築と3次元計測-多組織要素の分画・統合・解析のための実験操作マニュアル-2007

    • Author(s)
      島津徳人編(青葉孝昭、南郷脩史監修)
    • Total Pages
      83
    • Publisher
      ラトックシステムエンジニアリング株式会社
    • Related Report
      2007 Annual Research Report
  • [Book] 新う蝕の科学2006

    • Author(s)
      青葉 孝昭 (浜田茂幸, 大嶋隆編)
    • Total Pages
      232
    • Publisher
      医歯薬出版
    • Related Report
      2006 Annual Research Report
  • [Book] 未来の歯科医師をめざして -集計データから浮かび上がる歯科学生の学び事情-2006

    • Author(s)
      青葉 孝昭
    • Total Pages
      158
    • Publisher
      医学晴報社
    • Related Report
      2006 Annual Research Report
  • [Book] 平成17年度病理学Web教材(学内LAN配信資料・冊子版)(青葉孝昭監修)2005

    • Author(s)
      日本歯科大学病理学講座編
    • Total Pages
      143
    • Publisher
      日本歯科大学出版
    • Related Report
      2005 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.ndu.ac.jp/~pathhome/index.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.ndu.ac.jp/~pathhome/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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