• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles of Toll-like receptors in bacterial phagocytosis

Research Project

Project/Area Number 17390498
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionHokkaido University

Principal Investigator

SHIBATA Ken-ichiro  Hokkaido University, Graduate School of Dental Medicine, Professor., 大学院歯学研究科, 教授 (50145265)

Co-Investigator(Kenkyū-buntansha) KUROKI Yoshio  Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (70161784)
YASUDA Motoaki  Hokkaido University, Graduate School of Dental Medicine, associate Professor., 大学院歯学研究科, 助教授 (90239765)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2006: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2005: ¥9,200,000 (Direct Cost: ¥9,200,000)
Keywordsphagocytosis / Toll-like receptor / FSL-1 / DC-SIGN / Dectin-1 / CD14 / Th2 response / 微生物貪食 / リポペプチドFSL-1 / 大腸菌由来LPS / TLR2
Research Abstract

Enormous lines of evidence have been accumulated that Toll-like receptors (TLRs) function as sensors for microbial invasion before uptake and degradation of bacteria. However, less is known about how signaling triggered by TLRs leads to phagocytosis of pathogens by phagocytes. This study was designed to determine whether stimulation of TLR2 with mainly the lipopeptide FSL-1 plays a role in phagocytosis of pathogens by macrophages. Diacylated lipopeptide FSL-1 markedly enhanced phagocytosis of S. aureus more strongly than that of E. coli; but did not enhance phagocytosis of latex beads. FSL-1 stimulation resulted in enhanced phagocytosis of bacteria by macrophages from TLR2+/+ mice but not those from TLR2-/-mice. Chinese hamster ovary cells stably expressing TLR2 failed to phagocytose these bacteria, but the cells expressing CD14 did. FSL-1 induced upregulation of the expression of phagocytic receptors including MSR1, CD36, DC-SIGN and Dectin-1 in THP-1 cells. Human embryonic kidney 293 cells transfected with DC-SIGN and MSR1 phagocytosed these bacteria. These results suggest that the FSL-1-induced enhancement of phagocytosis of bacteria by macrophages may be explained partially by the upregulation of scavenger receptors and the C-type lectins through TLR2-mediated signaling pathways and that TLR2 by itself does not function as a phagocytic receptor.
This study also demonstrated that CD14 and the C-type lectin DC-SIGN enhances the recognition of lignads by TLR2 and FSL-1 induced Th2 response in vivo.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (12 results)

All 2007 2006 2005

All Journal Article (12 results)

  • [Journal Article] The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway2007

    • Author(s)
      Mae M, Iyori M, Yasuda M, Shamsul H, Kataoka H, Hasebe A, Kiura K, Totsuka Y, Shibata K
    • Journal Title

      FEMS Immunology and Medical Microbiology 49

      Pages: 398-409

    • NAID

      120002451616

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The diacylated lipopeptide FSL-1 enhances phagocytosis of bacteria by macrophages through Toll-like receptor 2-mediated signaling pathway.2007

    • Author(s)
      Mae M, Iyori M, Yasuda M, Shamsul H, Kataoka H, Hasebe A, Kiura K, Totsuka Y, Shibata K.
    • Journal Title

      FEMS Immunol. Med. Microbiol 49

      Pages: 398-409

    • NAID

      120002451616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the receptor complex of Toll-like receptors 2 and 1 without binding to the complex2006

    • Author(s)
      Nakata, M.Yasuda, M.Fujita, H.Kataoka, K.Kiura, H.Sano, K.Shibata^*
    • Journal Title

      Cellular Microbiology 8

      Pages: 1899-1909

    • NAID

      120000955848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses2006

    • Author(s)
      K.Kiura, H.Kataoka, M.Yasuda, N.Inoue, K.Shibata
    • Journal Title

      FEMS Immunology and Medical Microbiology 48

      Pages: 44-55

    • NAID

      120002451618

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The synthetic analogue of mycoplasmal lipoprotein FSL-1 induces dendritic cell maturation through TLR22006

    • Author(s)
      Kiura K., Kataoka H., Nakata T., Into T., Yasuda M., Akira S., Inoue N., Shibata K.
    • Journal Title

      FEMS Immunology and Medical Microbiology 46

      Pages: 78-84

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of N-linked glycans in the recognition of microbial lipopeptides and lipoproteins by TLR22006

    • Author(s)
      Kataoka H., Yasuda M., Iyori M., Kiura K., Narita M., Nakata T., Shibata K.
    • Journal Title

      Cellular Microbiology 8

      Pages: 1199-1207

    • NAID

      120000955850

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the receptor complex of Toll-like receptors 2 and 1 without binding to the complex.2006

    • Author(s)
      T.Nakata, M.Yasuda, M.Fujita, H.Kataoka, K.Kiura, H.Sano, K.Shibata*.
    • Journal Title

      Cell. Microbiol 8

      Pages: 1899-1909

    • NAID

      120000955848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The diacylated lipopeptide FSL-1 induces TLR2-mediated Th2 responses.2006

    • Author(s)
      K.Kiura, H.Kataoka, M.Yasuda, N.Inoue, K.Shibata*.
    • Journal Title

      FEMS Immunol. Med. Microbiol 48

      Pages: 44-55

    • NAID

      120002451618

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The synthetic analogue of mycoplasmal lipoprotein FSL-1 induces dendritic cell maturation through TLR2.2006

    • Author(s)
      Kiura K., Kataoka H., Nakata T., Into T., Yasuda M.Akira S., Inoue N., Shibata K.
    • Journal Title

      FEMS Immunol. Med. Microbiol 46

      Pages: 78-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of N-linked glycans in the recognition of microbial lipopeptides and lipoproteins by TLR2.2006

    • Author(s)
      Kataoka H., Yasuda M., Iyori M., Kiura K., Narita M., Nakata T., Shibata K.
    • Journal Title

      Cell Microbiol 8

      Pages: 1199-1207

    • NAID

      120000955850

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] CD14 directly binds to triacylated lipopeptides and facilitates recognition of the lipopeptides by the receptor complex of Toll-like receptors 2 and 1 without binding to the complex2006

    • Author(s)
      T.Nakata, M.Yasuda, M.Fujita, H.Kataoka, K.Kiura, H.Sano, K.Shibata
    • Journal Title

      Cellular Microbiology 8

      Pages: 1899-1909

    • NAID

      120000955848

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Apoptosis signal-regulating kinase 1 (ASK1)-mediated sustained p38 MAPK activation regulates mycoplasmal lipoprotein- and staphylococcal peptidoglycan-triggered Toll-like receptor 2 signaling pathways.2005

    • Author(s)
      Into T, Shibata K.
    • Journal Title

      Cellular Microbiology 7・9

      Pages: 1305-1317

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi