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New Approach to the Study on Oral Biofilm Infectious Diseases by Metagenomic Analysis

Research Project

Project/Area Number 17390502
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionOkayama University

Principal Investigator

FUKUI Kazuhiro  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (70034171)

Co-Investigator(Kenkyū-buntansha) KOKEGUCHI Susumu  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor (10144776)
TANIMOTO Ichiro  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (00280686)
TAKASHIBA Shogo  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (50226768)
MAEDA Hiroshi  Okayama University, Hospital, Lecturer (00274001)
KARIYAMA Reiko  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (40112148)
玉木 直文  岡山大学, 大学院・医歯薬学総合研究科, 助手 (20335615)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,260,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2006: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 2005: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsBacteria / Metagenome / Biofilm / Infectious Disease / ゲノム
Research Abstract

Oral microflora has been investigated so far with cultivation-based techniques. Over 700 species of bacteria have been previously estimated in oral cavity but only about 50% of them have been cultivated. Any understanding of the oral environment requires knowledge of the entire bacterial community. The uncultivated and as-yet-uncharacterized bacterial species may participate in the etiology of oral diseases. To resolve this problem, we devised an approach by the metagenomic analysis based on the bacterial 16S ribosomal RNA gene (16S rDNA) .
In this study, we determined the profiles, composition and changes of various oral microflora using culture-independent molecular methods, i.e., clone library method, polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) method and terminal restriction fragment length polymorphism analysis (T-RFLP) method. We also established the high-resolution, real-time and three-dimensional imaging system on the biofilm structure and deve … More lopment in the modified capillary flow-cell using confocal laser scanning microscopy with fluorescence visualization supporting by Professor Philip S. Stewart (Center for Biofilm Engineering at Montana State University).
We analyzed the microbial profiles and composition of tonsilloliths, which are potential cause of oral malodor by 16S rDNAs based clone library method. The isolated partial 16S rDNA sequences (approximately 600bp) were analyzed. Anaerobic bacteria detected in tonsilloliths, belonged to the genera Fusobacterium, Eubacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, which appear to be associated with production of volatile sulfur compounds. We further examined the effectiveness of professional toothbrushing on microflora changes in subgingival plaques by PCR-DGGE analysis. PCR-DGGE analysis revealed that professional toothbrushing resulted in a decrease in the number of periodontal pathogens and the dramatic change of microflora in subgingival plaques.
We also revealed that Archaea was frequently isolated from deep periodontal pockets in Japanese patients with periodontitis and pathogenic and opportunistic bacterial species were frequently detected in the patients receiving bone marrow transplantation after chemotherapy. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (40 results)

All 2008 2007 2006 2005 Other

All Journal Article (26 results) (of which Peer Reviewed: 8 results) Presentation (9 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Rapid and simple detection of major eight periodontal pathogens by loop-mediated isothermal amplification method.2008

    • Author(s)
      Miyagawa J, Maeda H, Murauchi T, et. al.
    • Journal Title

      FEMS Immunology and Medical Microbiology (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Rapid and simple detection of major eight periodontal pathogens by loop-mediated isothermal amplification method2008

    • Author(s)
      Miyagawa, J., Maeda, H., Murauchi, T., Kokeguchi, S., Tanimoto, I., Nishimura, F., Fukui, K., Takashiba, S
    • Journal Title

      FEMS Immunology and Medical Microbiology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 緑膿菌性尿路感染症対策としての抗バイオフィルム剤探索とその基盤技術の開発2007

    • Author(s)
      狩山玲子, 門田晃一, 公文裕巳
    • Journal Title

      第41回緑膿菌感染症研究会講演記録 41

      Pages: 39-43

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease2007

    • Author(s)
      Yamazaki K, Honda T, et. al.
    • Journal Title

      Clinical and Experimental Immunology 149(3)

      Pages: 445-452

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 大腸菌性バイオフィルム形成抑制活性を有するクランベリー尿中代謝物の探索2007

    • Author(s)
      狩山玲子, 大西令子, 伊東秀之, 吉田隆志, 波多野力, 苔口進, 等
    • Journal Title

      Bacterial Adherence & Biofilm 20巻(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 歯学部学生のメチシリン耐性ブドウ球菌の保菌調査と院内感染に関する意識調査について2007

    • Author(s)
      渡辺朱理, 佐藤法仁, 苔口進, 福井一博
    • Journal Title

      日本歯科衛生学会雑誌 1巻・2号

      Pages: 25-33

    • NAID

      40015392692

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genome-wide screening of genes required for swarming motility in Escherichia coli K-122007

    • Author(s)
      Inoue T, Shingaki R, Hirose S, Waki K, Mori H, Fukui K
    • Journal Title

      J.Bacteriol. 189巻・3号

      Pages: 950-7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 大腸菌性バイオフィルム形成抑制活性を有するクランベリー尿中代謝物の探索2006

    • Author(s)
      狩山玲子, 大西令子, 伊東秀之等
    • Journal Title

      Bacterial Adherence & Biofilm 20

      Pages: 129-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Periodontal infection and dyslipidemia in type 2 diabetics: association with increased HMG-CoA reductase expression.2006

    • Author(s)
      Nishimura F, Taniguchi A, et. al.
    • Journal Title

      Horm Metab Res. 38(8)

      Pages: 530-535

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Composition of the bacterial flora in tonsilloliths.2006

    • Author(s)
      Tsuneishi M, Yamamoto T, Kokeguchi S, et. al.
    • Journal Title

      Microbes & Infection 8(9-10)

      Pages: 2384-2389

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of urinary metabolites of cranberry on biofilm formation by Eseherichia coil isolated from a patient with urinary tract infection2006

    • Author(s)
      Kariyama, R, Ohnishi, R., Ito, H., Yoshida, T., Hatano, T., Kokeguchi, S., Mitsuhata, R., Wada, K., Monden, K., Kumon, H
    • Journal Title

      Bacterial Adherence & Biofilm 20

      Pages: 129-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Periodontal infection and dyslipidemia in type 2 diabetics : association with increased HMG-CoA reductase expression.2006

    • Author(s)
      Nishimura, F., Taniguchi, A., Yamaguchi-Morimoto, M., Soga, Y., Iwamoto, Y., Kokeguchi, S., Kuroe, A., Fukushima, M., Nakai, Y., Seino, Y
    • Journal Title

      Horm Metab Res 38(8)

      Pages: 530-535

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Composition of the bacterial flora in tonsilloliths2006

    • Author(s)
      Tsuneishi, M., Yamamoto, T., Kokeguchi, S., Tamaki, N., Fukui, K., Watanabe, T
    • Journal Title

      Microbes Infect 8(9-10)

      Pages: 2384-2389

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Composition of the bacterial flora tonsilloliths2006

    • Author(s)
      Tsuneishi M, Yamamoto T, Kokeguchi S, Tamaki N, Fukui K, Watanabe T
    • Journal Title

      Microbes Infect. 8巻・9-10号

      Pages: 2384-9

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Periodontal infection and dyslipidemia in type 2 diabetics : association with increased HMG-CoA reductase expression2006

    • Author(s)
      Nishimura F, Taniguchi A, Yamaguchi-Morimoto M, Soga Y, Iwamoto Y, Kokeguchi S, et al.
    • Journal Title

      Horm Metab Res. 38巻・8号

      Pages: 530-5

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Leukotoxin production by Actinobacillus actionmycetemcomitans2006

    • Author(s)
      Inoue T, Fukui K, Ohta H
    • Journal Title

      Toxin Reviews 25巻・1号

      Pages: 1-18

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene polymorphisms in periodontal health and disease.2006

    • Author(s)
      Takashiba S, Naruishi K.
    • Journal Title

      Periodontology 2000 40巻

      Pages: 94-106

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 難培養微生物群集へのメタゲノム解析によるアプローチ2005

    • Author(s)
      苔口進, 前田博史, 玉木直文, 福井一博
    • Journal Title

      日本細菌学雑誌 60(3)

      Pages: 491-494

    • NAID

      130001386150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 口腔微生物フローラ研究と保健2005

    • Author(s)
      苔口進, 前田博史
    • Journal Title

      Food & Food Ingredients Journal of Japan 210(4)

      Pages: 348-360

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] New approach to uncultivable microbial communities by metagenomic analysis2005

    • Author(s)
      Kokeguchi, S., Maeda, H., Tamaki, N., Fukui, K
    • Journal Title

      Nippon Saikingaku Zasshi 60(3)

      Pages: 491-494

    • NAID

      130001386150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Oral Microflora and Their Relation to Health and Disease2005

    • Author(s)
      Kokeguchi, S., Maeda, H
    • Journal Title

      Food & Food Ingredients Journal of Japan 210(4)

      Pages: 348-360

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 難培微生物群集へのメタゲノム解析によるアプローチ2005

    • Author(s)
      苔口進, 前田博史, 玉木直文, 福井一博
    • Journal Title

      日本細菌学雑誌 60巻・3号

      Pages: 491-494

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 口腔微生物フローラ研究と保健2005

    • Author(s)
      苔口進, 前田博史
    • Journal Title

      FOOD & FOOD INGREDIENTS JOURNAL OF JAPAN 210巻・4号

      Pages: 348-360

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 腸球菌性尿路バイオフィルム形成に関する検討2005

    • Author(s)
      狩山玲子, 瀬野祐子, 光畑律子, 門田晃一, 公文裕巳
    • Journal Title

      Bacterial Adherence & Biofilm 19巻

      Pages: 60-65

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 走査型電子顕微鏡による膿栓細菌叢の観察2005

    • Author(s)
      恒石美登里, 玉木直文, 山本龍生, 渡邊達夫
    • Journal Title

      口腔衛生学会誌 55巻・4号

      Pages: 337-337

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 日本人歯周病患者からの古細菌の検出および同定2005

    • Author(s)
      山部こころ, 前田博史, 苔口進, 園井教裕, 吉住和歌子等
    • Journal Title

      日本歯科保存学雑誌 48巻・春季特別号

      Pages: 125-125

    • Related Report
      2005 Annual Research Report
  • [Presentation] Change in the Oral Bacterial Microflora of Patients Receiving Bone Marrow Transplantation.2007

    • Author(s)
      Hassan Wael、谷本一郎、前田博史等
    • Organizer
      日本歯科保存学会 2007年度 秋季学術大会(第127回)
    • Place of Presentation
      岡山コンベンションセンター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Archaeal Infection in Japanese Patients with Petiodontitis.2007

    • Author(s)
      K. Yamabe, H. Maeda, S. Kokeguchi, et. al.
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Up-regulation of IL-6 production from adipocytes by LPS-stimulated Macrophage.2007

    • Author(s)
      A. Yamashita, F. Nishimura, Y. Soga, et. al.
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Changes in Oral bacterial Microflora of patients Receiving Bone Marrow Transplantation.2007

    • Author(s)
      Hassan, Wael, Tanimoto, Ichiro, Maeda, Hiroshi, Sugiura, Yuko, Soga, Yoshihiko, Kokeguchi, Susumu, Takashiba, Shogo
    • Organizer
      Autumn Meeting in 2007 (the 127th) of The Japanese Society of Conservative Dentistry
    • Place of Presentation
      Okayama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Archaeal Infection in Japanese Patients with Periodontitis2007

    • Author(s)
      K., Yamabe, H., Maeda, S., Kokeguchi, T., Murauchi, I., Tanimoto, S., Takashiba
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Up-regulation of IL-6 production from adipocytes by LPS-stimulated Macrophage2007

    • Author(s)
      A., Yamashita, F., Nishimura, Y., Soga, Y., Iwamoto, S., Yoshizawa, H., Iwamoto, S., Kokeguchi, S., Takashiba
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      New Orleans, Louisiana, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Change in the Oral Bacterial Microflora of Patients Receiving Bone Marrow Transplantation2007

    • Author(s)
      Hassan Wael、谷本一郎、前田博史等
    • Organizer
      日本歯科保存学会 2007年度 秋季学術大会(第127回)
    • Place of Presentation
      岡山コンベンションセンター
    • Related Report
      2007 Annual Research Report
  • [Presentation] Biofilm in urinary tract infections-the search for antibiofilm agents.2005

    • Author(s)
      Kariyama, R.
    • Organizer
      24th International Congress of Chemotherapy
    • Place of Presentation
      Manila, Philippines
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] (Invited speaker) : Biofilm in urinary tract infections- the search for antibiofilm agents2005

    • Author(s)
      Kariyama, R
    • Organizer
      24th International Congress of Chemotherapy
    • Place of Presentation
      Manila, Philippines
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Preventive Periodontology臨床を支えるサイエンスを知る・唾液検査を活用する・2007

    • Author(s)
      前田博史、苔口 進、高柴正悟
    • Total Pages
      373
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.geocities.jp/okayama_u_dent_saikin/result.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.okayama-u.ac.jp/user/med/Gyoseki/index.html

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.geocities.jp/okayama_u_dent_saikin/result.html

    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.okayama-u.ac.jp/user/med/Gyoseki/index.html

    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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