Development of periodontal ligament regeneration therapy using novel extracellular matrix
| Project/Area Number |
17390510
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University (2006-2007)
Kanagawa Dental College (2005) |
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Principal Investigator |
SAITO Masahiro Osaka University, Dentistry, Assistant professor (40215562)
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| Co-Investigator(Kenkyū-buntansha) |
TERANAKA Toshio Kanagawa Dental College, Dentistry, Professor (60104460)
NOZAKI Naohito Kanagawa Dental College, Dentistry, Assistant Professor (70222198)
HATA Ryuichiro Kanagawa Dental College, Dentistry, Professor (10014276)
KIYONO Tohru Osaka University, Protein Institute, professor (10186356)
関口 清俊 大阪大学, 蛋白質研究所, 教授 (50187845)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥9,400,000 (Direct Cost: ¥9,400,000)
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| Keywords | Periodontal ligament / Development / extracellular matrix / Regeneration / Tissue engineering / ligament / Organogenesis / elastic fiber / 歯胚 / マルファン症候群 / 歯周病 / マイクロフィブリル / 微小線維 |
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| Research Abstract |
The periodontal ligament (PDL) is a strong connective tissue that surrounds the tooth root, absorbs occlusal forces, and functions as a sense organ. The structure of the PDL is often irreversibly damaged when chronic inflammation in the form of "periodontitis" develops, affecting the periodontium. Although various treatments are available for periodontitis, reliable regeneration of the PDL is not yet possible. A basic understanding of PDL development at the molecular level is required to develop methods to regenerate damaged PDL, since the regeneration process will need to mimic the cellular events of PDL development. However, since the specific marker genes for PDL is not available, molecular mechanisms of PDL development have not yet been clarified. Previously we found that the novel extracellular protein ADAMTSL-4 is specifically expressed in PDL. In the present study, we examined if ADAMTSL-4 involved in the PDL development. Expression patterning analysis revealed that adamts14□ mRNA is strongly expressed in the dental follicle, the origin of the PDL. Immunohistochemical and electron microscopy analysis revealed that oxytalan fibrillar-like localization of ADAMTSL4 in the adult PDL. Overexpression of ADAMTSL4 in PDL cells significantly induced formation of oxytalan fiber-like microfibril assembly, indicating that ADAMTSL4 involved in the formation of oxytalan fiber. Our results suggest that ADAMTSL4□ regulates microfibril assembly of fibrillin-1 during PDL development, and could be a novel therapeutic target for the periodontitis.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Neurotrophic factor NT-4 regulates ameloblastin expression via full-length TrkB.2008
Author(s)
K., Yoshizaki, S., Yamamoto, A., Yamada, K., Yuasa, T., Iwamoto, E., Fukumoto., H., Harada, M., Saito, A., Nakasima, K., Nonaka, Y., Yamada, S., Fukumoto
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Journal Title
J Biol Chem 283
Pages: 3385-3391
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] JB Uney, IA Phylactou, 2007 Twist induces reversal of myotube formation2007
Author(s)
E, Hjiantoniou, M, Anayasa, P, Nicolaou, I, Bantounas, M, Saito, S, Iseki
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Journal Title
Differentiation 76(2)
Pages: 182-92
Description
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[Journal Article] Association of TIMP-2 with extracellular matrix exposed to mechanical stress and its co-distribution with periostin during mouse mandible development2007
Author(s)
N., Yoshiba, K., Yoshiba, A., Hosoya, M., Saito, T., Yokoi, T., Okiji, N., Amizuka, H., Ozawa
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Journal Title
Cell Tissue Res 330(1)
Pages: 133-45
Description
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[Journal Article] PLAP-1/Asporin, a Novel Negative Regulator of Periodontal Ligament Mineralization2007
Author(s)
S., Yamada, M., Tomoeda, Y., Ozawa, S., Yoneda, Y., Terashima, K., Ikezawa, S., Ikezawa, M., Saito, S., Toyosawa, S., Murakami
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Journal Title
J Biol Chem 282(32)
Pages: 23070-23080
Description
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[Journal Article] Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis2007
Author(s)
T., Yamashiro, L., Zheng, Y., Shitaku, M., Saito, T., Tsubakimoto, K., Takada, T., Takano-Yamamoto, I., Thesleff
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Journal Title
Differentiation 75(5)
Pages: 452-62
Description
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[Journal Article] The development of a bioengineered organ germ method2007
Author(s)
K., Nakao, R., Morita, Y., Saji, K., Ishida, Y., Tomita, M., Ogawa, M., Saito, Y., Ibmooka, T., Tsuji
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Journal Title
Nature Method 4(3)
Pages: 227-30
Description
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[Journal Article] Establishment of Immortalized Dental Follicle Cells for Generating Periodontal Ligament In Viva2007
Author(s)
T., Yokoi, M., Saito, T., Kiyono, S., Iseki, K., Kosaka, E., Nishida, T., Tsubakimoto, H., Harada, K., Eto, T., Noguchi, T., Thranaka
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Journal Title
Cell Tissue Res 327(2)
Pages: 301-311
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcriptome Database KK-Periome for Periodontal Ligament Development : Expression Profiles of the Extracellular Matrix Genes2007
Author(s)
E., Nishida, T., Sasaki, S., Kazuko Ishikawa, K., Kosaka, M., Aino, T., Noguchi, T., Thranaka, N., Shimizu, M., Saito
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Journal Title
Gene 404(1-2)
Pages: 70-79
Description
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[Presentation] Establishment of transcriptome database for analyzing periodontal ligament development2007
Author(s)
Masahiro, Saito Eisaku, Nishida, Takashi, Sasaki, Sabine, K, Ishikawa, Nobuyoshi, Shimizu, Toshiyuki, Yoneda
Organizer
1st Asian Biomaterials Congress
Place of Presentation
Tsukuba
Year and Date
2007-12-06
Description
「研究成果報告書概要(欧文)」より
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