|Budget Amount *help
¥16,410,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥810,000)
Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2005: ¥10,200,000 (Direct Cost: ¥10,200,000)
In order to identify candidate genes associated with salivary gland tumors and to detect tumor bio-markers for clinical tumor characters, many tumor samples of pleomorphic adenoma, adeno-carcinoma, and adenoid cystic carcinoma were examined using combined comparative genomic hybridization (CGH), microarray (Affymetrix GeneChipTM), and two dimensional difference in-gel electrophoresis (2D-DIGE)-MALDI-TOF/MS- peptide mass fingerprinting (PMF) methods."Gain" and "loss" legion were detected on many sites of chromosomes and the pattern of those were specific to the pathological type of the tumors. Microarray analysis and proteomic approach revealed over-expression of stathmin, maspin, SIGLEC 8, SERPINB2, BOK, LGALS7, ANXA8, FAT3, CDKN1A, FBN2, and IGFBP2 genes and down-expression of ECHS 1, SOD 2, ALAD, Proapolipoprotein, SERPIN B1, NALP1, CASP8, CD28, and ATP2B2 genes in the salivary gland tumors. Then, we focused on stathmin and maspin, Examination of many clinical specimens confirmed the over-expression of those genes and proteins in the salivary gland tumors. These results suggested that stathmin is involved in the carcinogenesis of both squamous cell carcinoma and salivary gland tumors and that maspin is specifically associated with differenciation and pathologic type of salivary gland tumors.