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The study of molecular and gene analysis and bone metabolism-improving effect of propiomibacterium freudenreichii milk whey culture against osteoporosis.

Research Project

Project/Area Number 17390538
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

SUGAHARA Toshio  Okayama University, Oral and Maxillofacial Reconstructive Surgery, Professor and Chairman, 大学院医歯薬学総合研究科, 教授 (10116048)

Co-Investigator(Kenkyū-buntansha) MISHIMA Katsuaki  Okayama University, Hospital, Associate Professor, 医学部・歯学部附属病院, 講師 (60304317)
UENO Takaaki  Okayama University, Oral and Maxillofacial Reconstructive Surgery, Assistant Professor, 大学院医歯薬学総合研究科, 助手 (60252996)
MIZUKAWA Nobuyoshi  Okayama University, Hospital, Associate Professor, 医学部・歯学部附属病院, 講師 (00263608)
YAMADA Tomohiro  Okayama University, Oral and Maxillofacial Reconstructive Surgery, Assistant Professor, 大学院医歯薬学総合研究科, 助手 (60335619)
YAMACHIKA Eiki  Okayama University, Oral and Maxillofacial Reconstructive Surgery, Assistant Professor, 大学院医歯薬学総合研究科, 助手 (10294422)
福永 城司  岡山大学, 大学院・医歯薬学総合研究科, 助手 (10284069)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 2005: ¥9,500,000 (Direct Cost: ¥9,500,000)
KeywordsOsteoporosis / Bifidobacteria / Propiomibacterium freudenreichii / DHNA / T-cell / B-cell / Naphthoquinone / Bone mass recovery / 免疫抑制剤 / T, B細胞 / FACS
Research Abstract

To see the relationship between immune cell and osteoporosis occurrence by immunosuppresant, we have established the osteoporosis mouse model using immunosuppresant (FK506) injected nude mouse and wild type mouse. The results of wild type were amount of trabecula bone under enchondral ossification was decreased, and number of osteoclast, TRAP positive, was obviously increased in enchondral ossification. On the other hand, there was almost no bone resorption under enchondral ossification in the nude mouse' s result. Those results suggest that immune cell contributes to the osteoporosis occurrence by the immunosuppresant. We have found propiomibacterium freudenreichii milk whey culture (PC), starter of Swiss cheese, could rescue osteoporosis in our model. In further experiment, PC could inhibit the differentiation of osteoclast and prevent osteoporosis. But it is hard to simply say that PC rescued osteoporosis in our model by differential inhibition of osteoclast, because of the osteocla … More st differentiation and immune system participation. To see the details, we have gotten the following results, a) The number of osteoclast was significantly increased in the mouse osteoblast-like stroma cell and osteoblast mixed culture with supplementation of CD4 positive T-cell or B220 positive B-cell, extracted from bone marrow with magnet beads. b) FACS result was the immunosuppresant decreased the number of T-cell and increased the number of B-cell in spleen and mesentericlymph node of our model, c) DHNA, contained in PC, has naphthoquinone. The basic skeleton is the same as vitamin K2, which is a research attention of bone mass recovery in recent years. DHNA made the same results as PC's and was identified as a factor of inhibitory effect of bone resorption.
We elucidated the bone mass recovery with DHNA, and the relationship immune system and osteoclast. Furthermore, to identify osteoporosis-related gene, we have seen the alteration of gene expression in'bone marrow with DNA microarray. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (23 results)

All 2006 2005

All Journal Article (21 results) Book (2 results)

  • [Journal Article] Expression of transcription factor Sox9 in the cartilage formation from grafted periosteal cells.2006

    • Author(s)
      Takaaki Ueno
    • Journal Title

      Asian Journal Oral Maxillofac 18 (1)

      Pages: 46-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hypoxic Regulation of Stability of Connective Tissue Growth Factor/CCN2 mRNA by 31-Untranslated Region Interacting with a Cellular Protein in Human Chondrosarcoma Cells.2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Oncogene 25

      Pages: 1099-1110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel angiogenic inhibitor DN-9693 that inhibits post-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial growth factor (VEGF) in human endothelial cells.2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Molecular Cancer Therapeutics 5

      Pages: 129-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of a Range Image for Facial Motion Analysis : a Method for Analyzing Lip Motion.2006

    • Author(s)
      Mishima K
    • Journal Title

      Computerized Medical Imaging and Graphics 30(1)

      Pages: 53-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of bone formation in grafted periosteam harvested from tibia and calvaria.2006

    • Author(s)
      Fujii T
    • Journal Title

      Microsc Res Tech. (On line Pub)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells.2006

    • Author(s)
      Yamada, T
    • Journal Title

      Asian J Oral Maxillofac Surg 18

      Pages: 89-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of transcription factor sox9 in the cartilage formation from grafted periosteal cells2006

    • Author(s)
      Takaaki Ueno
    • Journal Title

      Asian journal of oral and maxillofacial surgery 18

      Pages: 35-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hypoxic Regulation of Stability of Connective Tissue Growth Factor/CCN2 mRNA by 3-Untranslated Region Interacting with a Cellular Protein in Human Chondrosarcoma Cells2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Oncogene 25

      Pages: 1099-1110

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Novel Angiogenic Inhibitor DN-9693 That Inhibits Posttranscriptional Induction of Connective Tissue Growth Factor (CTGF/CCN2) by Vascular Endothelial Growth Factor in Human Endothelial Cells2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Molecular Cancer Therapeutics 5(1)

      Pages: 129-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Production of a Range Image for Facial Motion Analysis : a Method for Analyzing Lip Motion2006

    • Author(s)
      Katsuaki Mishima
    • Journal Title

      Computerized Medical Imaging and Graphics 30(1)

      Pages: 53-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of bone formation in grafted periosteum harvested from tibia and calvaria2006

    • Author(s)
      Takashi Fujii
    • Journal Title

      Microsc Res. Tech, On line Pub

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells2006

    • Author(s)
      Tomohiro Yamada
    • Journal Title

      Asian journal of oral and maxillofacial surgery 18

      Pages: 89-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Kodomo wa sugarashii (otousan to Okaasan no kosodate no tameni) (in Japanese)2006

    • Author(s)
      Toshio Sugahara
    • Journal Title

      Kanehara & Co., Ltd 579

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of transcription factor Sox9 in the cartilage formation from grafted periosteal cells2006

    • Author(s)
      Takaaki Ueno
    • Journal Title

      Asian Journal Oral Maxillofac 18(1)

      Pages: 46-51

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hypoxic Regulation of Stability of Connective Tissue Growth Factor/CCN2 mRNA by 3ユ-Untranslated Region Interacting with a Cellular Protein in Human Chondrosarcoma Cells.2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Oncogene 25

      Pages: 1099-1110

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Novel angiogenic inhibitor DN-9693 that inhibits Past-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial grow factor (VEGF) in human endothelial cells.2006

    • Author(s)
      Seiji Kondo
    • Journal Title

      Molecular Cancer Therapeutics 5

      Pages: 129-137

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Production of a Range Image for facial Motion Analysis : a Method for Analyzing Lip Motion2006

    • Author(s)
      Mishima K
    • Journal Title

      Computerized Medical Imaging and Graphics 30(1)

      Pages: 53-59

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of bone formation in grafted periosteum Harvested from tibia and calvaria2006

    • Author(s)
      Fujii T
    • Journal Title

      Microsc Res Tech. (On line Pub)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of 2,3,7,8-teracholrldibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells.2006

    • Author(s)
      Yamada T
    • Journal Title

      Asian J Oral axillrfac Surg 18

      Pages: 89-94

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Various immunostaining patterns of CD31,CD34 and endoglin and their relationship with lymph node metastasis in oral squamous cell carcinomas2005

    • Author(s)
      Hitoshi Nagatsuka
    • Journal Title

      J Oral Pathol Med 34

      Pages: 70-76

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Heparanase gene and protein expression in ameloblastoma : possible role in local invasion of tumor cells2005

    • Author(s)
      Hitoshi Nagatsuka
    • Journal Title

      Oral Oncology 41

      Pages: 542-548

    • Related Report
      2005 Annual Research Report
  • [Book] 子どもは素晴らしい(お父さんとお母さんの子育てのために)2006

    • Author(s)
      菅原 利夫 共著
    • Total Pages
      579
    • Publisher
      金原出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 子どもは素晴らしい (お父さんとお母さんの子育てのために)2006

    • Author(s)
      菅原 利夫 共著
    • Total Pages
      579
    • Publisher
      金原出版株式会社
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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