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Mechanical stress-generated expression of MMPs in human PDL cell

Research Project

Project/Area Number 17390557
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionOsaka University (2007)
Matsumoto Dental University (2005-2006)

Principal Investigator

UEMATSU Setsuko  Osaka University, Dental Hospital, Senior lecturer (80271378)

Co-Investigator(Kenkyū-buntansha) HIRAOKA Yukihiro  Matsumoto Dental University, Graduate School, Professor (20097512)
UEMATSU Takashi  Matsumoto Dental University, Graduate School, Associate professor (40203476)
USUI Yohei  Matsumoto Dental University, School ofDentistry, Assistant professor (00387424)
大嶋 嘉久  松本歯科大学, 歯学部, 助手 (70340057)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,750,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsIL-17 / T cell / periodontal ligament cell / MMP-1 / mechanical stress
Research Abstract

T cell were subjected to mechanical stress (MS) for periods ranging from 0-24h, via the use of a Flexell Strain Unit at 37℃ in a humidified 5% CO_2 atmosphere. After the stimulation, cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. IL-17 secretion did not differ significantly between MS treated cells and control cells, but amount of IL-17 was slightly increased in MS cells. The effects of MS on IL-17A mRNA expressions were investigated by RT-PCR analysis. Mechanical stress with a Flexell Strain Unit is a useful form of mechanical stress to simulate T cell functioning under normal metabolism, but the amount of MS applied might not be suitable for detecting the expression of T cell cytokines, which was realized to have important functions in inflammatory responses.
The effects of MS on periodontal ligament (PDL) cells were indicated. Th … More e mRNA expressions of other cytokines and mediators related to bone remodeling during orthodontic treatment were investigated by RT-PCR analysis. IL-1β mRNA expression was showed steady state level on 0-4h, but was increased after 6hr and keep the expression until 24hr. MMP-1 mRNA expression was not changed under control condition and investigated on 2-24hr. IL-17RA mRNA increased the expression after addition of MS.
Intermittent tension force induced IL-1β mRNA expression but synthesis. This mechanical force also stimuli MMP-1 mRNA expression. MMP-1 mRNA expression was not induced by IL-1β, and it may be affected by intermittent mechanical force alone or other cytokines which were induced synthesis by mechanical forces. After the stimulation of IL-17, PDL cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. MMP-1 and IL-1β secretion differ between IL-17 treated cells and controls. MMP-1 mRNA was increased after 24 hours and kept steady state level until 72hr. IL-1β mRNA was found to be increased after 24 hours and decreased at 72hr. This point will be investigated in detail. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 2005 Other

All Journal Article (16 results) (of which Peer Reviewed: 7 results) Presentation (7 results)

  • [Journal Article] Inorganic polyphosphate: a possible stimulant of bone formation.2007

    • Author(s)
      Hacchou Y, et. al.
    • Journal Title

      J Dent Res 86

      Pages: 893-897

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Change in maximum occlusal force in association with maxillofacial growth2007

    • Author(s)
      Usui T, et. al.
    • Journal Title

      Orthod Craniofac Res 10

      Pages: 226-234

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inorganic polyphosphate : a possible stimulant of bone formation2007

    • Author(s)
      Hacchou, Y, et. al.
    • Journal Title

      J Dent Res 86

      Pages: 893-897

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Change in maximum occlusal force in association with maxillofacial growth2007

    • Author(s)
      Usui, T, et. al.
    • Journal Title

      Orthod Craniofac Res 10

      Pages: 226-236

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inorganic polyphosphate: a possible stimulant of bone formation.2007

    • Author(s)
      Hacchou Y.
    • Journal Title

      J Dent Res 86

      Pages: 893-897

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Change in maximum occlusal force in association with maxillofacial growth.2007

    • Author(s)
      Usui T.
    • Journal Title

      Orthod Craniofac Res 10

      Pages: 226-234

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Refractory factors in head and neck cancer: ATP binding cassette trans porters expressed in head and neck cancer cell lines.2006

    • Author(s)
      Uematsu T, et. al.
    • Journal Title

      Oral Sci Int 3

      Pages: 72-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Multidrug resistance-associated protein 7 expression is involved in cross-resistance to docetaxel in salivary gland adenocarcinoma cell lines.2006

    • Author(s)
      Naramoto H, et. al.
    • Journal Title

      Int J Oncol 30

      Pages: 393-401

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Refractory factors in head and neck cancer : ATP binding cassette transporters expressed in head and neck cancer cell lines2006

    • Author(s)
      Uematsu, T, et. al.
    • Journal Title

      Oral Sci Int 3

      Pages: 72-83

    • NAID

      10020357283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Multidrug resistance-associated protein 7 expression is involved in cross-resistance to docetaxel in salivary gland adenocarcinoma cell lines2006

    • Author(s)
      Naramoto, H, et. al.
    • Journal Title

      Int J Oncol 30

      Pages: 393-341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Refractory factors in head and neck cancer : ATP binding cassette transporters expressed in head and neck cancer cell lines.2006

    • Author(s)
      Uematsu T et al.
    • Journal Title

      Oral Sci Int 3・2

      Pages: 72-83

    • NAID

      10020357283

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Multidrug resistance-associated protein 7 expression is involved in cross-resistance to docetaxel in salivary gland adenocarcinoma cell lines.2006

    • Author(s)
      Naramoto H et al.
    • Journal Title

      Int J Oncol 30・2

      Pages: 393-401

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prostaglandin E2 strongly inhibits human osteoclast formation.2005

    • Author(s)
      Take I, et. al.
    • Journal Title

      Endocrinology 146

      Pages: 5204-5214

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prostaglandin E2 strongly inhibits human osteoclast formation2005

    • Author(s)
      Take, I, et. al.
    • Journal Title

      Endocrinology 146

      Pages: 5204-5214

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prostaglandin E2 strongly inhibits human osteoclast formation.2005

    • Author(s)
      Take I et al.
    • Journal Title

      Endocrinology 146・12

      Pages: 5204-5214

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 顎外固定装置を下顎大臼歯の遠心移動に用いた成人骨格性下顎前突症例2005

    • Author(s)
      薄井 他
    • Journal Title

      松本歯学 31

      Pages: 256-262

    • NAID

      120004428324

    • Related Report
      2005 Annual Research Report
  • [Presentation] Effect of inorganic polyphosphate on osteoblastic and periodontal ligament cells2006

    • Author(s)
      S. UEMATSU, et. al.
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      ボルチモア、アメリカ合衆国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Replantation with or without cryopreservation of rat molars2006

    • Author(s)
      S. KURIHARA, et. al.
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      ボルチモア、アメリカ合衆国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanical stress-generated expression of MMPs and IL-1β in ratfibroblasts2006

    • Author(s)
      S. UEMATSU, et. al.
    • Organizer
      85th General Session & Exhibition of the IADR
    • Place of Presentation
      ボルチモア、アメリカ合衆国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ポリリン酸は骨形成を促進する2006

    • Author(s)
      内橋, ら
    • Organizer
      日本骨代謝学会学術集会(25回)
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of inorganic polyphosphate on osteoblastic and periodontal ligament cells

    • Author(s)
      S., Uematsu, Y., Usui, H., NAKAYAMA, T., UEMATSU, K., FURUSAWA, S., KURIHARA, M., YAMAOKA
    • Organizer
      85th General Session & Exhibition of the IADR
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Replantation with or without cryopreservation of rat molars

    • Author(s)
      S., KURIHARA, T, TANAKA, Y., USUI, Y., OSHIMA, S, UEMATSU, N., OKAFUJI
    • Organizer
      85th General Session & Exhibition of the IADR
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanical stress-generated expression of MMPs and IL-1b in rat fibroblasts

    • Author(s)
      S., UEMATSU, Y., OSHIMA, T., UEMATSU, K., FURUSAWA, S., KURIHARA
    • Organizer
      85th General Session & Exhibition of the IADR
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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