| Project/Area Number |
17390557
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Osaka University (2007)
Matsumoto Dental University (2005-2006) |
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Principal Investigator |
UEMATSU Setsuko Osaka University, Dental Hospital, Senior lecturer (80271378)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Yukihiro Matsumoto Dental University, Graduate School, Professor (20097512)
UEMATSU Takashi Matsumoto Dental University, Graduate School, Associate professor (40203476)
USUI Yohei Matsumoto Dental University, School ofDentistry, Assistant professor (00387424)
大嶋 嘉久 松本歯科大学, 歯学部, 助手 (70340057)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,750,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2005: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | IL-17 / T cell / periodontal ligament cell / MMP-1 / mechanical stress |
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| Research Abstract |
T cell were subjected to mechanical stress (MS) for periods ranging from 0-24h, via the use of a Flexell Strain Unit at 37℃ in a humidified 5% CO_2 atmosphere. After the stimulation, cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. IL-17 secretion did not differ significantly between MS treated cells and control cells, but amount of IL-17 was slightly increased in MS cells. The effects of MS on IL-17A mRNA expressions were investigated by RT-PCR analysis. Mechanical stress with a Flexell Strain Unit is a useful form of mechanical stress to simulate T cell functioning under normal metabolism, but the amount of MS applied might not be suitable for detecting the expression of T cell cytokines, which was realized to have important functions in inflammatory responses.
The effects of MS on periodontal ligament (PDL) cells were indicated. Th
… More
e mRNA expressions of other cytokines and mediators related to bone remodeling during orthodontic treatment were investigated by RT-PCR analysis. IL-1β mRNA expression was showed steady state level on 0-4h, but was increased after 6hr and keep the expression until 24hr. MMP-1 mRNA expression was not changed under control condition and investigated on 2-24hr. IL-17RA mRNA increased the expression after addition of MS.
Intermittent tension force induced IL-1β mRNA expression but synthesis. This mechanical force also stimuli MMP-1 mRNA expression. MMP-1 mRNA expression was not induced by IL-1β, and it may be affected by intermittent mechanical force alone or other cytokines which were induced synthesis by mechanical forces. After the stimulation of IL-17, PDL cells were collected for RNA extraction and condition culture medium was collected and stored until use in enzyme-linked immune sorbent assay (ELISA). Control samples were also collected in the same way. MMP-1 and IL-1β secretion differ between IL-17 treated cells and controls. MMP-1 mRNA was increased after 24 hours and kept steady state level until 72hr. IL-1β mRNA was found to be increased after 24 hours and decreased at 72hr. This point will be investigated in detail. Less
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