Development of customized periodontal tissue regenerative therapy by transtiptome analysis of periodontal ligament
| Project/Area Number |
17390560
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Shinya Osaka University, Graduate School ofDentistry, Professor (70239490)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMABUKURO Yoshio Osaka University, Graduate School of Dentistry, Associate Professor (50231361)
YAMADA Satoru Osaka University, Dental Hospital, Associate Professor (40359849)
HASHIKAWA Tomoko Osaka University, Graduate School ofDentistry, Assistant Professor (00362682)
HATTORI Masahira Kitasato University, Kitasato Institute for Life Sciences, Professor (70175537)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,180,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | periodontal ligament / periodontal tissue / periodontal tissue regeneration / genome science / transcriptome / 完全長cDNAライブラリ / PLAP-1 |
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| Research Abstract |
Utilizing human periodontal ligament full-length cDNA library, which was prepared by G-cap method, we have determined DNA sequences of approximately 20,000 clones, which had been randomly selected. Basset on the information we have established the full-length cDNA database of human periodontal ligament, which was named FuLPerioGen. Among 87 genes whose expression frequencies were more than 39, we have identified 9 novel genes.
We also carried out the functional analysis of Periodontal ligament associated protein (PLAP)-1/asporin, one of periodontal ligament specific genes identified in the above-mentioned database. As a result, it was revealed that PLAP-1/asporin negatively regulated the BMR-2-induced expression of osterix and alkaline phosphatase activity and that this inhibition was caused by molecular interaction between BMP-2 and PLAP-1/asporin. Interestingly, we have clarified that leucine rich repeat (LRR) motif, especially LRR5, in PLAP-1/asporin protein played an important role
… More
in the interaction.
Focusing on the biological activities of basic fibroblast growth factor (FGF-2), we analyzed exhacellular matrix production regulated by FGF-2. We clarified that stimulation with FGF-2 upregulated the expression of hyaluronan synthase (HAS) and osteopontin. Interestingly, we revealed that those molecules play an important role for cell migration of periodontal ligament rolls in early phase of periodontal tissue regeneration process.
In conclusion, we succeeded in establishing FuLPerioGen database, which was the full-length cDNA database of human periodontal ligament. This database included highly expressed novel genes, which may be closely associated with the biological events of periodontal tissue regeneration. In addition, we revealed that same molecules in FuLPerioGen such as PLAP-1/asporin and osteopontin played crucial roles during the course of periodontal tissue regeneration. The results obtained in this study would provide valuable intimation on molecular basis of periodontal tissue regeneration. Less
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Basic fibroblast growth factor regulates expression of heparan sulfate in human periodontal ligament cells2008
Author(s)
Y., Shimabukuro, T., Ichikawa, Y., Terashima, T., lwayama, H., Oohara, T., Kajikawa, R., Kobayashi, H., Terashima, M., Takedachi, M., Terakura, T., Hashikawa, S., Yamada, S., Murakami
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Journal Title
Matrix Biol 27
Pages: 232-241
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells2008
Author(s)
Y., Terashima, Y., Shimabukuro, H., Terashima, M., Ozasa, M., Terakura, K., Ikezawa, T., Hashikawa, M., Takedachi, H., Oohara, S., Yamada, S., Murakami
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Journal Title
J. Cell. Physiol (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif2008
Author(s)
M., Tomoeda, S., Yamada S, H., Shirai, Y., Ozawa, M., Yanagita S., Murakami
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Journal Title
Biochem Biophys Res Commun 371
Pages: 197-196
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] PLAP-1/asporin : A novel negative regulator of periodontal ligament mineralization. J. Biol. Chem, 282 : 23070-23080, 20072007
Author(s)
S., Yamada, M., Tomoeda, Y., Ozawa, S., Yoneda, Y., Terashima, K., Ikezawa. S., Ikegawa, M., Saito, S., Toyosawa, S., Murakami
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Journal Title
J. Biol. Chem 262
Pages: 23070-23080
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Regulation of PLAP-1 expression in periodontal ligament cells2006
Author(s)
S., Yamada, Y., Ozawa Y., M., Tomoeda, R., Matoba, K., Matsubara, S., Murakami
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Journal Title
J. Dent. Res 85
Pages: 447-451
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Regulation of PLAP-1 Expression in Periodontal Ligament Cell.2006
Author(s)
Yamada, S., Ozawa, Y, Tomoeda, M., Matoba, R., Matsubara, K., Murakami, S.
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Journal Title
Journal of Dental Research 85(in press)
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