• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular basis of periodontal ligament stem cells by bioinformatics

Research Project

Project/Area Number 17390561
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

YAMADA Satoru  Osaka University, Dental Hospital, Assistant Professor (40359849)

Co-Investigator(Kenkyū-buntansha) NOZAKI Takenori  Osaka University, Graduate Pcbceil of Dentistry, Assistant Professor (30263304)
HASHIKAWA Tomoko  Osaka University, Graduate School of Dentisay, Assistant Professor (00362682)
YANAGITA Manabu  Osaka University, Graduate School of Dentistty, Assistant Professor (80379081)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsPLAP-1 / DNA chip analysis / periodontal ligament stem cells / IDI / 歯根膜細胞 / 硬組織形成分化 / オリゴDNAチップ
Research Abstract

We took three different approaches to reveal molecular and genetical mechanisms of periodontal ligament stem cell functions in this study. First approach is to investigate molecular mechanisms of homeostasis in in vivo periodontal ligament tissue. We focused on PLAP-1, which is periodontal ligament specific protein. In this study we demonstrated that PLAP-1 acts as an negative regulator of cytodifferenfiation and mineralization by regulating BMP-2 activity to prevent periodontal ligament from developing non-physiological mineralization, such as ankylosis. These results help us to understand one of complicated mechanisms of homeostasis in periodontal ligament tissue.
Second approach is bioinformatic analysis utilizing large scale transcriptome experiments by DNA chip. We analyzed gene expression during the cytodifferentiation and mineralization of periodontal ligament cells in vitro by DNA chip. We found that more than 3,000 genes were regulated during the cybdifthrentiation of periodont … More al ligament cells. We constructed gene expression profile database in which we can perform gene ontology search and see gene pathway analysis. As a result of data mining, we found that Idl gene of which function in periodontal ligament was unknown, was down-regulated during the cytodifferentiation. We performed functional analysis about Idl and revealed that Idl regulated cytodifferentiation and mineralization of periodontal ligament cells.
Third approach is transcriptome analysis of periodontal ligament stem cells. We isolated SP (side population) cells from primary cultured human periodontal ligament cells by Fluorescence-activated cell sorting (FACS). We then, performed DNA chip analysis of gene expression in SP cells. We foud several genes were differentially expressed in SP cells derived from periodontal ligament compared to MP (major population) cells which were thought to be non-stem cell population. This gene expression profiling of SP cells derived from periodontal ligament helps us to understand more details of molecular characteristics of periodontal stem cell functions. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2008 2007 2006 2005 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Book (1 results)

  • [Journal Article] PLAP-1/asporin inhibits activation of BMP receptor via its leucine rich repeat motif2008

    • Author(s)
      Tomoeda, M, Yamada, S, Shirai, S, Ozawa, IT, Yanagita, M, Murakami, S
    • Journal Title

      Biochem. Biophys. Res. Commun. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] PLAP-1/asporin, a novel negative regulator of periodontal ligament mineralization.2007

    • Author(s)
      Yamada, et. al.
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 23070-23080

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] PLAP-1/asporin, a novel negative regulator of periodontal ligament mineralization2007

    • Author(s)
      Yamada, S, Tomoeda, M, Ozawa, Y, Yoneda, S, Terashima, Y, Ikezawa, K, Ikegawa, S, Saito, M, Toyosawa, S, Murakami, S
    • Journal Title

      J Biol Chem 282(32)

      Pages: 23070-80

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] PLAP-1/asporin: A novel negative regulator of periodontal ligament mineralization.2007

    • Author(s)
      S. Yamada
    • Journal Title

      J. Biol. Chem. 282

      Pages: 23070-23080

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of PLAP-1 expression in periodontal ligament cells.2006

    • Author(s)
      Yamada, et. al.
    • Journal Title

      Journal of Dental Research 85

      Pages: 447-451

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Regulation of PLAP-1 expression in periodontal ligament cell2006

    • Author(s)
      Yamada, S, Ozawa, Y, Tomoeda, M, Matoba, R, Matsubara, K, Murakami, S
    • Journal Title

      J Dent Res 85(5)

      Pages: 447-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Regulation of PLAP-1 expression in periodontal ligament cells2006

    • Author(s)
      S.YAMADA, Y.OZAWA, M.TOMOEDA, R.MATOBA, K.MATSUBARA, S.MURAKAMI
    • Journal Title

      Journal of Dental Research 85

      Pages: 447-451

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Regulation of PLAP-1 Expression in Periodontal Ligament Cell.2006

    • Author(s)
      Yamada, S., Ozawa, Y, Tomoeda, M, Matoba, R., Matsubara, K., Murakami, S.
    • Journal Title

      Journal of Dental Research 85(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] PLAP-1/asporin, inhibits activation of BMP receptor via its leucine rich repeat motif.

    • Author(s)
      Tomoeda, et. al.
    • Journal Title

      Biochem. Biophys. Res. Commun. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Presentation] 歯根膜特異的遺伝子PLAP-1の単離と解析2007

    • Author(s)
      山田 聡
    • Organizer
      第30回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanical stress-induced glutamate signaling in periodontal ligament cells2007

    • Author(s)
      Fujihara, C, Yamada, S, Yoneda, S, Tauchi, T, Kajikawa, T, Ozawa, Y, Murakami, S
    • Organizer
      55th JADR
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-11-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヒト歯根膜細胞の全遺伝子発現プロファイリング解析により同定された遺伝子の機能解析2007

    • Author(s)
      米田晋也
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      大宮
    • Year and Date
      2007-06-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] PLAP-1 : a novel negative regulator of mineralization of periodontal ligament2006

    • Author(s)
      Yamada, S, Tomoeda, M, Ozawa, Y, Saito, M, Murakami, S
    • Organizer
      Annual Meeting of American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2006-09-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification and characterization of periodontal ligament specific genes2006

    • Author(s)
      Yamada, S, Murakami, S
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Pisa, Italy
    • Year and Date
      2006-06-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Regulation of cytodifferentiation of periodontal ligament cells by SFRP42005

    • Author(s)
      Yamada, S, Suzuki, S, Tomoeda, M, Yoneda, S, Murakami, S
    • Organizer
      53rd JADR
    • Place of Presentation
      Okayama, Japan
    • Year and Date
      2005-11-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular basis of periodontal ligament : identification and characterization of periodontal ligament specific genes2005

    • Author(s)
      Yamada, S, Murakami, S
    • Organizer
      Osaka University- Kyungpook University international symposium
    • Place of Presentation
      Tegu, Korea
    • Year and Date
      2005-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 歯の再生-歯の発生生物学から歯の再生研究まで-2006

    • Author(s)
      山田 聡、村上伸也
    • Publisher
      真興交易(株)医書出版部
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi