Epidemiological survey and classification of genotypes of intestinal parasitic protozoa Blastocystis
| Project/Area Number |
17406007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Nara Women's University |
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Principal Investigator |
YOSHIKAWA Hisao Nara Women's University, Faculty of Science, Associate Professor (50191557)
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| Co-Investigator(Kenkyū-buntansha) |
WU Zhiliang Gifu University, Graduate school of Medicine, Assistant Professor (90313874)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Blastocystis / epidemiology / intestinal parasite / protozoa / genotype / polymolphism / 疫学 / ゲノム / 人畜共通感染性 |
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| Research Abstract |
Although, pathogenic potential of an intestinal parasite, Blastocystis hominis, had been proposed on the basis of clinical symptom, the true nature of the parasite infection in humans is still controversial. Since B. hominis is known to be genetically polymorphic, it is generally assumed that the certain genotype of the parasite may contribute to its pathogenic potential. Based on these backgrounds, this project was performed on the survey of human Blastocystis infection and determined genotypes among Blastocystis populations. Since the prevalence of human Blastocystis infection in Japan was lower than other developing Asian countries, this project was performed in Thailand, China and Singapore. Finally, a total 47 isolates were obtained from 2 different epidemiological setting in Thailand. In China, 270 isolates were obtained from 4 different epidemiological setting. In Singapore, only 9 isolates were obtained from a major hospital in Singapore. When 270 isolates in China was screened by PCR with our developed sequenced tagged-site primers, all of the isolates except for an isolate were classified into the known genotypes. Since an isolate was negative with any STS primers, entire sequence of the small subunit rRNA gene was sequence and phylogenetically analyzed with the known genotypes. Interestingly, an human isolates was positioned in an additional new clade with other 2 isolates reported in GenBank.
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Report
(4 results)
Research Products
(57 results)
