| Project/Area Number |
17406013
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Virology
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| Research Institution | Oita University (2006-2007)
Nagasaki University (2005) |
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Principal Investigator |
KAMRUDDIN Ahmed Oita University, Institute of Scientific Research, Associate Professor (00398140)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIZONO Akira Oita University, Faculty of Medicine, Professor (70218155)
中込 治 長崎大学, 大学院・医歯薬学総合研究科, 教授 (70143047)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥13,120,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | rotavirus / enidemiolocy / serotvne / reassortant / Turkey / rotavirus / epidemiology / serotype / reassortant / Turkey |
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| Research Abstract |
In the present project we found that the epidemiology of rotavirus gastroenteritis in Turkey has both components of developed and developing countries. During the 2004-2005 period serotype G1 was dominant and in that period emergence of serotype G9 was first detected in Turkey. A new phylogenetic lineage of G9 rotaviruses has been detected in a rotavirus from Turkey. During the 2005 2007 period G1 was still dominant however G9 was eliminated and G2 has been emerging in Turkey.
The exact epidemiology of other group of rotavirus infection is not known in Turkey. We found that the prevalence of group C rotavirus is 0.4% only. The nucleotide sequences of VP7, VP6 and NSP4 genes showed that the Turkish group C rotavirus is a human virus and not an animal virus.
We experienced a patient with intussusception caused by G9 rotavirus. The case was resolved naturally without surgical intervention. This report confirming that natural rotavirus infection may cause intussusception.
Viremia during rotavirus. infection in human is an unresolved issue. Five serum samples from rotavirus infected patients with antigenemia were subjected to ultracentrifugation in the anticipation that if rotavirus is present in serum it should be in the precipitate. We did electron microscopy of this precipitates however no' virus was observed. Different cytokines and chemokines were determined in the serum of rotavirus infected patient with and without antigenemia in the anticipation that if there is viremia during infection there should be a difference between the two groups, however no difference was detected. These results indicate that possibly viremia does not occur during rotavirus gastroenteritis. Comparison of serum viral RNA load with the level of antigen in the serum might confirm our findings.
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