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Live cell imaging of radial glial cells of the cerebral cortex

Research Project

Project/Area Number 17500200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionNara Institute of Science and Technology (2007)
University of Tsukuba (2005-2006)

Principal Investigator

HATANAKA Yumiko  Nara Institute of Science and Technology, Graduate School of Material Sciences, Researcher (40271548)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsNeurosicence / Cell and Tissue / Neuroanatomy / Development and Differentication / Cell-cell interaction / 神経発生 / ラジアルグリア / 大脳皮質 / 神経細胞移動 / 可視化 / 細胞形態 / タイムラプス
Research Abstract

Radial glial cells arise early from the neuroepithelium and show a highly polarized structure. Their cell bodies are located in the ventricular or subventricular zone, and their long radial processes span the neural tube, ending at the pial surface with conical endfeet. In the developing cerebral cortex, they serve as neuronal progenitors and as scaffolds for migration of post-mitotic neurons. Although it is supposed that cell-to-cell interactions of radial glial cells with the neurons are important for guiding, even more promoting the migration, dynamic behavior of radial glial cells during this period remains largely unknown. To address this issue, we developed a system for live cell imaging for radial glial cells. We examined a several types of membrane anchored eYFP (enhanced yellow fluorescent protein), and found that eYFP-CAAX, which has k-Ras farnesylation sequence, uniformly labeled radial glial cell surface. According to the method for Morin et al. (Nat Neurosci 10, 1440, 2007), we applied the Cre-loxP system using a combination of plasmid vectors to label a limited number of radial glial cells by electroporation. With a 10^<-4>-10^<-5>: 1 ratio of Cre to loxP plasmids enabled us to examine each radial glial cell morphology. Analyses of labeled radial glial cells in fixed preparations at embryonic day 13.5, 16.5, or 19 (= postnatal day 0) indicated their smooth cell surface with occasional protrusions or branches throughout the radial process except near the pial surface. The terminal branches of radial fiber emerged, extended and increased in number as development proceeded. High concentration of oxygen facilitated the survival of labeled cell in organotypic slice culture and enabled us to examine their behavior over 16 hours under a confocal microscope. These technical improvements will allowed us to analyze detailed behavior of radial glial cells during neuronal migration.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (16 results)

All 2009 2008 2006 2005

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (8 results)

  • [Journal Article] Distinct Roles of Neuropilin 1 Signaling for Radial and Tangential Exttension of Callosal Axons2009

    • Author(s)
      Hatanaka et. al.
    • Journal Title

      Journal of Comparative Neurology 514

      Pages: 214-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Distinct Roles of Neuropilin 1 Signaling for Radial and Tangential Extension of Callosal Axons.2009

    • Author(s)
      Hatanaka Y., Matsumoto T., Yanagawa Y., Fujisawa H., Murakami F., Masu M.
    • Journal Title

      Journal of Comparative Neurology 514

      Pages: 214-229

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation agter midline crossing2008

    • Author(s)
      Tanama, da et. al.
    • Journal Title

      Neural Development 3

      Pages: 29-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of RhoA in activity-dependent cortical axon branching2008

    • Author(s)
      Ohnami, et. al.
    • Journal Title

      Journal of Neuroscience 28

      Pages: 9117-9121

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradul up-regulated proteolytic activities2008

    • Author(s)
      Tamura, et. al.
    • Journal Title

      Journal of Neuroscience Methods 173

      Pages: 114-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing.2008

    • Author(s)
      Tamada A., Kumada T., Zhu Y., Matsumoto T., Hatanaka Y., Muguruma K., Chen Z., Tanabe Y., Torigoe M., Yamauchi K., Oyama H., Murakami F.
    • Journal Title

      Neural Development 3

      Pages: 29-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of RhoAin activity-dependent cortical axon branching.2008

    • Author(s)
      Ohnami S., Endo M., Hirai S., Uesaka N., Hatanaka Y, Yamashita T., Yamamoto N.
    • Journal Title

      Journal of Neuroscience 28

      Pages: 9117-9121

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities.2008

    • Author(s)
      Tamura H., Ng D. C., Tokuda T., Honda N., Nakagawa T., Mizuno T., Hatanaka Y., Ishikawa Y., Ohta J., Shiosaka S.
    • Journal Title

      Journal of Neuroscience Methods 173

      Pages: 114-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 大脳皮質錐体細胞の軸索ガイダンスにおけるニューロピリン1の役割について2008

    • Author(s)
      畠中由美子
    • Organizer
      基礎生物学研究所研究会「大脳皮質の発生と可塑性」
    • Place of Presentation
      岡崎
    • Year and Date
      2008-03-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A role of neuropilinl in axonal guidance of cortical pyramidal neurons.2008

    • Author(s)
      Hatanaka, Y.
    • Organizer
      Cortical Development and Plasticity II
    • Place of Presentation
      Okazaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 大脳皮質神経上皮細胞の増殖に対するR-spondin2の役割の解析2006

    • Author(s)
      畠中由美子, 他3名
    • Organizer
      第30回日本神経科学大会
    • Place of Presentation
      京都
    • Year and Date
      2006-07-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of a role of Rspondin2 on proliferation of the cortical neuroepithelium.2006

    • Author(s)
      Hatanaka, Y., Yamaguchi, M., Murakami, F., Masu, M.
    • Organizer
      The 30th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-07-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of gene expression changes in terminal phase of radial migration in the cerebral cortex2006

    • Author(s)
      畠中由美子
    • Organizer
      基礎生物学研究所研究会「大脳皮質の発生と機能構築」
    • Place of Presentation
      岡崎
    • Year and Date
      2006-03-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of gene expression changes in terminal phase of radial migration in the cerebral cortex.2006

    • Author(s)
      Hatanaka, Y.
    • Organizer
      Cortical Development and Plasticity I
    • Place of Presentation
      Okazaki
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 発生過程における海馬特異的遺伝子R-spondm2の機能解析2005

    • Author(s)
      畠中由美子, 他3名
    • Organizer
      第29回日本神経科学大
    • Place of Presentation
      横浜
    • Year and Date
      2005-07-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] R-spondin2 potentially regulates the size of the hippocampus.2005

    • Author(s)
      Hatanaka Y., Yamaguchi, M., Murakami, F., Masu, M.
    • Organizer
      The 29th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2005-07-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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