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Spontaneous depolarization waves of multiple origins in the embryonic rat CNS : Optical imaging with voltage-sensitive dyes.

Research Project

Project/Area Number 17500206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SATO Yoko  Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (70251501)

Co-Investigator(Kenkyū-buntansha) SATO Kastushige  Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (80291342)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsoptical imaging / voltage-sensitive dye / central nervous system / functiogenesis / rat embryo / chick embryo / spontaneous activity / depolarization wave
Research Abstract

During development, correlated neuronal activity plays an important role in the establishment of the central nervous system (CNS). We recently reported that in the embryonic rat CNS, a widely-propagating correlated neuronal activity, termed the depolarization wave, is evoked by various sensory nerve inputs. A remarkable feature of the depolarization wave is how it spread broadly through the brain and spinal cord. In the present study, we examined whether the depolarization wave occurs spontaneously in the embryonic rat CNS, and if so, where it originates. In E15-E16 rat embryos, spontaneous optical signals appeared in association with the rhythmic discharges of cranial motoneurons, and propagated through the brain and spinal cord with similar characteristics to the evoked depolarization wave. The spontaneous wave originated in multiple locations including two restricted regions located in the dorsomedial pons and rostral ventrolateral medulla as well as the media/caudal medulla and spinal cord. When the spinal cord was intact, the wave at E16 mainly originated in the lumbosacral region, although a wave associated with the oscillatory event was also generated in the cervical cord/caudal medulla. In preparations in which the spinal cord was transected, the contribution of the pons and medulla was more significant. These results show that the depolarization wave is triggered by the spontaneous activity of multiple neuronal populations which distribute widely from the pons to the lumbosacral cord. The network possibly behaves as a self-distributing system that maintains the incidence and complicated patterns of the correlated activity in the developing CNS.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (37 results)

All 2007 2006 2005 Other

All Journal Article (37 results)

  • [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., et al.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      European Journal of Neuroscience (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki, T. et al.
    • Journal Title

      Brain Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M, Sato, K.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., Kinoshita, M., Momose-Sato, Y.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GABA-induced intracellular Ca~<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., et al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      European Journal of Neuroscience (,in press.)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki, T. et al.
    • Journal Title

      Brain Research (,in press.)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1949-1962

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission.2006

    • Author(s)
      Ren, J., et al.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Optical recording of vagal pathway formation in the embryonic brainstem.2006

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical 126-127

      Pages: 39-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F. et al.
    • Journal Title

      BMC Neuroscience 7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission.2006

    • Author(s)
      Ren, J., Momose-Sato, Y., Sato, K., Greer, J.J.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y., Glover, J.C., Sato, K.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1949-1962

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F., Komine, O., Sato, K., Kanamatsu, T., Uchimura, M., Tanaka, K., Ohki-Hamazaki, H.
    • Journal Title

      BMC Neuroscience 7

      Pages: 75-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y., et al.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1949-1962

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F., et al.
    • Journal Title

      BMC Neuroscience 7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission2006

    • Author(s)
      Ren, J.et al.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Optical recording of vagal pathway formation in the embryonic brainstem2006

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging.2005

    • Author(s)
      Sato, K., et al.
    • Journal Title

      NeuroImage 25

      Pages: 1292-1301

    • NAID

      130007039125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS.2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 94

      Pages: 1606-1622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery.2005

    • Author(s)
      Nariai, T. et al.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe.2005

    • Author(s)
      Wang, S. et al.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording.2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Membrane Biology 208

      Pages: 183-191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging.2005

    • Author(s)
      Sato, K., 10 T., Tanaka, Y., Maehara, T., Miyakawa, N., Sasaki, S., Momose-Sato, Y., Ohno, K.
    • Journal Title

      NeuroImage 35

      Pages: 1292-1301

    • NAID

      130007039125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS.2005

    • Author(s)
      Momose-Sato, Y., Honda, Y., Sasaki, H., Sato, K.
    • Journal Title

      Journal of Neurophysiology 94

      Pages: 1606-1622

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery.2005

    • Author(s)
      Nariai, T., Sato, T., Hirakawa, K., Ohta, Y., Tanaka, Y., Ishiwata, K., Ishii, K., Kamino, K., Ohno, K.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe.2005

    • Author(s)
      Wang, S., Zhang, S., Sato, K., Srinivasan, M.V.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording.2005

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Journal of Membrane Biology 208

      Pages: 183-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 94

      Pages: 1606-1622

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging2005

    • Author(s)
      Sato, K. et al.
    • Journal Title

      NeuroImage 25

      Pages: 1292-1301

    • NAID

      130007039125

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery2005

    • Author(s)
      Nariai, T. et al.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe2005

    • Author(s)
      Wang, S. et al.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording2005

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Journal of Membrane Biology 208(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K.
    • Journal Title

      European Journal of Neuroscience (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.

    • Author(s)
      Sasaki, T., Nariai, T., Maehara, T., Sato, K., Oda, K., Ishii K.
    • Journal Title

      Brain Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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