Spontaneous depolarization waves of multiple origins in the embryonic rat CNS : Optical imaging with voltage-sensitive dyes.
| Project/Area Number |
17500206
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SATO Yoko Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (70251501)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kastushige Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (80291342)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | optical imaging / voltage-sensitive dye / central nervous system / functiogenesis / rat embryo / chick embryo / spontaneous activity / depolarization wave |
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| Research Abstract |
During development, correlated neuronal activity plays an important role in the establishment of the central nervous system (CNS). We recently reported that in the embryonic rat CNS, a widely-propagating correlated neuronal activity, termed the depolarization wave, is evoked by various sensory nerve inputs. A remarkable feature of the depolarization wave is how it spread broadly through the brain and spinal cord. In the present study, we examined whether the depolarization wave occurs spontaneously in the embryonic rat CNS, and if so, where it originates. In E15-E16 rat embryos, spontaneous optical signals appeared in association with the rhythmic discharges of cranial motoneurons, and propagated through the brain and spinal cord with similar characteristics to the evoked depolarization wave. The spontaneous wave originated in multiple locations including two restricted regions located in the dorsomedial pons and rostral ventrolateral medulla as well as the media/caudal medulla and spinal cord. When the spinal cord was intact, the wave at E16 mainly originated in the lumbosacral region, although a wave associated with the oscillatory event was also generated in the cervical cord/caudal medulla. In preparations in which the spinal cord was transected, the contribution of the pons and medulla was more significant. These results show that the depolarization wave is triggered by the spontaneous activity of multiple neuronal populations which distribute widely from the pons to the lumbosacral cord. The network possibly behaves as a self-distributing system that maintains the incidence and complicated patterns of the correlated activity in the developing CNS.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006
Author(s)
Maekawa, F., Komine, O., Sato, K., Kanamatsu, T., Uchimura, M., Tanaka, K., Ohki-Hamazaki, H.
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Journal Title
BMC Neuroscience 7
Pages: 75-87
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging.2005
Author(s)
Sato, K., 10 T., Tanaka, Y., Maehara, T., Miyakawa, N., Sasaki, S., Momose-Sato, Y., Ohno, K.
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Journal Title
NeuroImage 35
Pages: 1292-1301
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery.2005
Author(s)
Nariai, T., Sato, T., Hirakawa, K., Ohta, Y., Tanaka, Y., Ishiwata, K., Ishii, K., Kamino, K., Ohno, K.
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Journal Title
Journal of Neurosurgery 103
Pages: 414-423
Description
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[Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.
Author(s)
Sasaki, T., Nariai, T., Maehara, T., Sato, K., Oda, K., Ishii K.
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Journal Title
Brain Research (in press)
Description
「研究成果報告書概要(欧文)」より
Related Report
