Exhaustive detection and analysis of gene expression associated with radial migration and differentiation in developing neocortical neurons
| Project/Area Number |
17500207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
FURUKAWA Tomonori (2006) Hamamatsu Univ. Sch. Med., Physiol., Res. Assistant, 医学部, リサーチアシスタント (60402369)
井上 浩一 (2005) 浜松医科大学, 医学部, 助手 (80345818)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Atsuo Hamamatsu Univ. Sch. Med., Physiol., Prof., 医学部, 教授 (50254272)
KUMADA Tatsuro Hamamatsu Univ. Sch. Med., Physiol., Res. Assoc., 医学部, 助手 (00402339)
窪田 寿彦 浜松医科大学, 医学部, 助手 (80377746)
古川 智範 浜松医科大学, 医学部, リサーチアシスタント (60402369)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | development / brain / migration / microarray / cell sorting / neuron |
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| Research Abstract |
To label cortical plate cells by EGFP, in utero electroporation was applied to ICR mice embryos in embryonic day 13 (E13). EGFP labeled cells migrated radially to pial surface (radial migration). After the transfection, ventricular zone of E15, intermediate zone of E16 and cortical plate of E18 were dissected from acute brain slices. The dissected slices were dispersed by using protease, papain. EGFP positive cells were isolated from the dispersed cells by means of optical cell sorting methods. The RNA acquired from the EGFP positive cells were amplified and then investigated exhaustively by microarray analysis. Sema6C and Syntaphilin expressed over three fold in intermediate zone than ventricular zone and cortical plate. To confirm the microarray results at protein level, the localization of Sema6C and Syntaphilin in cortex was observed immunohistochemically. Sema6C and Syntaphilin were localized in intermediate zone in E17 mice. These results suggested that the expression of Sema6C and Syntaphilin in radially migrating cells were increased in intermediate zone. As it is known that Sema6C and Syntaphilin associates with cell migration, Sema6C and Syntaphilin could have functional role for radial migration in intermediate zone.
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Report
(3 results)
Research Products
(30 results)
