• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation and Analysis of Excitatory Neuronal Cell Death by Protein Transduction Therapy

Research Project

Project/Area Number 17500212
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionMitsubishi Kagaku Institute of Life Sciences

Principal Investigator

MATSUSHITA Masayuki  Mitsubishi Kagaku Institute of Life Sciences, Brain Stroke Resarch Group, Principal Investigator, 研究部門・脳梗塞研究グループ, 主任研究員 (30273965)

Co-Investigator(Kenkyū-buntansha) SAITOH Ken  Mitsubishi Kagaku Institute of Life Sciences, Brain Stroke Research Group, Postdoctoral Researcher, 研究部門・脳梗塞研究グループ, 特別研究員 (70426584)
富澤 一仁  岡山大学, 大学院・医歯薬総合研究科, 助教授 (40274287)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsProtein transduction system / PTD / Calpain / TRPM7 / Neuron / Apoptosis / Ischemia / NMDA / 脳梗塞 / 神経細胞死 / タンパク質導入 / カルシウム
Research Abstract

With the elucidation of the human genome, exhaustive analysis of genomic data related to gene transcription and the structure and function of translated protein products has progressed rapidly. Delivery of proteins and their functional domains or inhibitory peptides directly into the neuron is ideal to treat excitatory neuronal cell death and related signal transduction s.
However, the protein is often entrapped inside macropinosomes in the cytoplasm. Therefore, high dose concentrations of the protein are needed for it to function effectively. To overcome this problem, in this project, polyarginine was linked with the NH(2)-terminal domain of influenza virus hemagglutinin-2 subunit (HA2), which is a pH-dependent fusogenic peptide that induces the lysis of membranes at low pH levels. We also designed the calpain and TRPM7 inhibitory peptide fused with protein transduction domain and examined the protective effect for ischemic neuronal cell death. These designed peptides were significantly protected against ischemic cell death. These data clearly show that protein transduction system is useful method for treat ischemic neuronal cell death.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (15 results)

All 2006 2005

All Journal Article (15 results)

  • [Journal Article] Charge screening by internal pH and polyvalent cations as a mechanism for activation, inhibition and rundown of TRPM7/MIC channels.2006

    • Author(s)
      Kozak JA
    • Journal Title

      J. Gen. Physiol 126

      Pages: 499-514

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Charge screening by internal pH and polyvalent cations as a mechanism for activation, inhibition and rundown of TRPM7/MIC channels.2006

    • Author(s)
      Kozak JA
    • Journal Title

      J.Gen.Physiol. 126

      Pages: 499-514

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Protein Transduction Technology2005

    • Author(s)
      Matsushita M
    • Journal Title

      J.Mol.Med 83

      Pages: 24-28

    • NAID

      120002446478

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The NH2-terminal of influenza virus hemagglutinin-2 subunit peptides enhance the anti-tumor potency of poly-arginine-mediated p53 protein transduction.2005

    • Author(s)
      Michiue H
    • Journal Title

      J. Biol. Chem. 280

      Pages: 8285-8289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Channel function dissociated from kinase activity and autophosphorylation in TRPM7/ChaK1.2005

    • Author(s)
      Matsushita M
    • Journal Title

      J. Biol. Chem. 280

      Pages: 20793-20803

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of NMDA receptors by calpain in cortical neurons.2005

    • Author(s)
      Wu HY
    • Journal Title

      J. Biol. Chem 280

      Pages: 21588-21593

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The NH2-terminal of influenza virus hemagglutinin-2 subunit peptides enhance the anti-tumor potency of poly-arginine-mediated p53 protein transduction.2005

    • Author(s)
      Michiue H
    • Journal Title

      J.Biol.Chem. 280

      Pages: 8285-8289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Channel function dissociated from kinase -activity and autophosphorylation in TRPM7/ChaKl.2005

    • Author(s)
      Matsushita M
    • Journal Title

      J.Biol.Chem. 280

      Pages: 20793-20803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of NMDA receptors by calpain in cortical neurons.2005

    • Author(s)
      Wu HY
    • Journal Title

      J.Biol.Chem 280

      Pages: 21588-21593

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ubiquitination-resistant p53 protein transduction therapy facilitates anti-cancer effect on the growth of human malignant glioma cells2005

    • Author(s)
      Michiue H
    • Journal Title

      FEBS Lett 579

      Pages: 3965-3969

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mechanism of PDX-1 protein transduction2005

    • Author(s)
      Noguchi H
    • Journal Title

      Biochem Biophys Res Commun 332

      Pages: 68-74

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of NMDA receptors by calpain in cortical neurons2005

    • Author(s)
      Wu HY
    • Journal Title

      J Biol Chem 280

      Pages: 21588-21593

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Channel function dissociated from kinase activity and autophosphorylation in TRPM7/ChaK12005

    • Author(s)
      Matsushita M
    • Journal Title

      J Biol Chem 280

      Pages: 20793-20803

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Calpain inhibitors prevent neuronal cell death and ameliorate motor disturbances after compression-induced spinal cord injury in rats2005

    • Author(s)
      Arataki S
    • Journal Title

      J Neurotrauma 22

      Pages: 398-406

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The NH2-terminal of influenza virus hemagglutinin-2 subunit peptides enhance the anti-tumor potency of poly-arginine-mediated p53 protein transduction2005

    • Author(s)
      Michiue H
    • Journal Title

      J Biol Chem 280

      Pages: 8285-8289

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi