• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the multifunctional signaling of estrogen using the visualization of estrogen responsible neurons

Research Project

Project/Area Number 17500215
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionNippon Medical School

Principal Investigator

HAMADA Tomohiro  Nippon Medical School, Faculty of Medicine, Assistant professor (90312058)

Co-Investigator(Kenkyū-buntansha) SAKUMA Yasuo  Nippon Medical School, Graduate school of Medicine, Professor (70094307)
KIYAMA Yuko  Nippon Medical School, Faculty of Medicine, lecturer (60234390)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsestrogen / transgenic / sex difference / SDN-POA / hippocampus / potassium current / area postrema / cardiovascular reaulation / エストロジェン受容体 / 遺伝子プロモーター / 性的二型核 / 性分化 / 電気生理 / 膜電位依存性チャネル
Research Abstract

Estrogen plays an essential role for reproduction, plasticity and neuroprotection to affect on the central nervous system. The purpose of the present study is to examine the mechanisms of estrogen action using the visualized neurons that are responsible to estrogen in the estrogen receptor gene promoter transgenic rats. Most important result is that EGFP expression under the control of estrogen receptor gene promoter is a specific marker for the sexually dimorphic nucleus of the preoptic area. Because sexual dimorphism of this nucleus is established by estrogen during the perinatal period, the new, live marker would contribute to clarify the molecular mechanisms of the sexual differentiation. On the other hand, EGFP expression is observed in the hippocampus that is noted function of estrogen for the plasticity and neurogenesis. We determined acute and chronic effects of physiological concentration of estrogen on the outward potassium currents in the primary cultured EGFP hippocampal neurons. Immature neurons showed the facilitated effect of 48 h treatment of estrogen, however, mature neurons exhibited the suppressive effect on the outward potassium currents. After the 48 h treatment of estrogen, bath application of 250 pM estrogen enhanced the potassium currents. These results suggest that estrogen influence the development and excitability of hippocampal neurons. One more thing to be noted is that there is the sex difference in the number of EGFP expressed cells in the area postrema and the nucleus tractus solitarius that are known to play an important role for cardiovascular regulation. Because hypertension is inhibited by estrogen in female, we could expect to clear the mechanisms of estrogen function on the cardiovascular regulation. Thus, we can show a number of the important results and we would like to study further to make clear the molecular mechanisms of estrogen function in the central nervous system.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (28 results)

All 2008 2007 2006 2005 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (18 results) Remarks (2 results)

  • [Journal Article] Site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats2008

    • Author(s)
      Xu Q., Hamada T., 他3名
    • Journal Title

      Neuroscinece Letters (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats2008

    • Author(s)
      Xu, Q., Hamada, T., Kiyama, R., Sakuma, Y., Wada-Kiyama, Y
    • Journal Title

      Neuroscience Letters (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Site-specific regulation of gene expression by estrogen in the hypothalamus of adult female rats2008

    • Author(s)
      Xu Q, Hamada T. Kiyama R.他計5名中2人目
    • Journal Title

      Neuroscience Letters (in press)(2008年2月受理)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo visualization of estrogen receptor alpha gene promoter activity2006

    • Author(s)
      Hamada T., Sakuma Y.
    • Journal Title

      Journal of Nippon Medical School 73

      Pages: 114-115

    • NAID

      130004146845

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] In vivo visualization of estrogen receptor alpha gene promoter activity2006

    • Author(s)
      Hamada, T., Sakuma, Y
    • Journal Title

      Journal of Nippon Medical School 73

      Pages: 114-115

    • NAID

      130004146845

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Visualizing forebrain-specific usage of an estrogen receptor alpha promoter for receptor downregulation in the rat2005

    • Author(s)
      Hamada T., Wada-Kiyama Y., Sakuma Y.
    • Journal Title

      Molecular Brain Research 139

      Pages: 42-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Visualizing forebrain-specific usage of an estrogen receptor alpha promoter for receptor downregulation in the rat2005

    • Author(s)
      Hamada, T., Wada-Kiyama, Y., Sakuma, Y
    • Journal Title

      Molecular Brain Research 139

      Pages: 42-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Visualizing forebrain-specific usage of an estrogen receptor alpha promoter for receptor downregulation in the rat.2005

    • Author(s)
      Hamada T, Wada-Kiyama Y, Sakuma Y
    • Journal Title

      Molecular Brain Research 139(1)

      Pages: 42-51

    • Related Report
      2005 Annual Research Report
  • [Presentation] エストロジェン受容体遺伝子プロモーターとランスジェニックラッと海馬ニューロンの電位依存性カリウム電流に対するエストロジェンの修飾2007

    • Author(s)
      濱田 知宏、Shan H.、佐久間 康夫
    • Organizer
      第30回日本神経科学大会(Neuro2007)
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-09-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] エストロジェン受容体遺伝子プロモータートランスジェニックラット海馬ニューロンの電位依存性カリウム電流に対するエストロジェンの修飾2007

    • Author(s)
      濱田知宏、Shan HL、佐久間康夫
    • Organizer
      第30回日本神経科学大会(Neuro2007)
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-09-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラット脳の性分化臨界期におけるエストロゲン応答遺伝子2007

    • Author(s)
      許 晴、濱田 知宏、木山 裕子、木山 亮一、佐久間 康夫
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2007-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] in vivoにおける視索前野性的二型核の可視化2007

    • Author(s)
      濱田 知宏、佐久間 康夫
    • Organizer
      第84回日本生理学会大会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2007-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vivo visualization of the sexually dimorphic nucleus of the preoptic area2007

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 84th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Osaka, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estrogen modulates voltage dependent potassium currents in the EGFP-tagged neurons in the hippocampus of estrogen receptor gene promoter transgenic rats2007

    • Author(s)
      Hamada, T., Shan, H., Sakuma, Y
    • Organizer
      The 30th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] in vivoにおける視索前野性的二型核の可視化2006

    • Author(s)
      濱田 知宏、佐久間 康夫
    • Organizer
      第33回日本神経内分泌学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2006-10-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estrogen receptor alpha gene promoter activity is a marker for the sexually dimorphic nucleus of the preoptic area2006

    • Author(s)
      Hamada T., Sakuma Y.
    • Organizer
      The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      JW Marriott Hotel,Seoul
    • Year and Date
      2006-10-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 視索前野性的二型核特異的新規マーカーとしてのエストロジェン受容体遺伝子プロモーター活性2006

    • Author(s)
      濱田 知宏、佐久間 康夫
    • Organizer
      第29回日本神経科学大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2006-07-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 成熟雌ラット視床下部における神経関連遺伝子のエストロゲン応答性2006

    • Author(s)
      許 晴、濱田 知宏、木山 裕子、木山 亮一、佐久間 康夫
    • Organizer
      第83回日本生理学会大会
    • Place of Presentation
      群馬県民会館
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 脳の性分化に関わるエストロジェン応答遺伝子発現のプロファイリング2006

    • Author(s)
      濱田 知宏、佐久間 康夫
    • Organizer
      第83回日本生理学会大会
    • Place of Presentation
      群馬県民会館
    • Year and Date
      2006-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Expression profiling of estrogen responsive genes for the sexual differentiation of the brain2006

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 83rd Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Maebashi, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estrogen receptor alpha gene promoter activity is a marker for the sexually dimorphic nucleus of the preoptic area2006

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 29th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kyoto, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estrogen receptor alpha gene promoter activity is a marker for the sexually dimorphic nucleus of the preoptic area2006

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      Seoul, Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vivo visualization of the sexually dimorphic nucleus of the preoptic area2006

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 33th Annual Meeting of the Japan Neuroendocrine Society
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 雌ラット視床下部におけるエストロゲン制御因子の解析2005

    • Author(s)
      許 晴、濱田 知宏、木山 裕子、木山 亮一、佐久間 康夫
    • Organizer
      第82回日本生理学会大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2005-05-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] GFPを指標とする脳内エストロジェン受容体遺伝子プロモーターの周生期発現2005

    • Author(s)
      濱田 知宏、佐久間 康夫
    • Organizer
      第82回日本生理学会大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2005-05-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Developmental changes in the usage of an estrogen receptor gene promoter in the rat brain2005

    • Author(s)
      Hamada, T., Sakuma, Y
    • Organizer
      The 82nd Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Sendai, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.nms.ac.jp/nms/seiri1/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.nms.ac.jp/nms/seiri1/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi