| Project/Area Number |
17500230
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Shimane University |
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Principal Investigator |
YASUI Yukihiko Shimane University, Dept. of Anatomy, Professor, 医学部, 教授 (30174501)
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| Co-Investigator(Kenkyū-buntansha) |
TSUMORI Toshiko Shimane University, Dept. of Anatomy, Assistant Professor, 医学部, 助手 (30217377)
岸 敏郎 島根大学, 医学部, 助教授 (80214766)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | lateral hypothalamus / amygdala / melanocortin 4 receptor / melanin-concentrating hormone / orexin / transgenic mouse / feeding behavior |
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| Research Abstract |
Orexin (ORX)-and melanin-concentrating hormone (MCH)-containing neurons as well as melanocortin 4 receptor (MC4-R)-expressing neurons in the lateral hypothalamus (LH) are involved in the regulation of feeding behavior. To know whether or not these neurons are under the influence of the amygdala, here we examine synaptic organization between these neurons and axon terminals originating from the central amygdaloid nucleus (CeA). MC4-R-expressing neurons were visualized as green fluorescent protein (GFP)-immunoreactive (ir) neurons using a transgenic mouse model in which GFP is produced under the control of the MC4-R promoter. After biotinylated dextran amine (BDA) injection into the CeA, overlapping distribution of BDA-labeled fibers and ORX-, MCH-, or GFP-ir neurons was seen in the LH region. In this region, BDA-labeled boutons were in contiguity with cell bodies and proximal dendrites of ORX-, MCH-, or GFP-ir neurons. Under the electron microscope, the CeA axon terminals formed asymmetrical synapses predominantly with somata and dendrites of MCH-and GFP-ir neurons, and occasionally with those of ORX-ir neurons.
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