• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The effect of steroid hormones on synaptogenesis and its mechanisms

Research Project

Project/Area Number 17500241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionToyo University (2006-2007)
St. Marianna University School of Medicine (2005)

Principal Investigator

OHTNAI Ritsuko  Toyo University, Department of Life Sciences, Professor (00161183)

Co-Investigator(Kenkyū-buntansha) MIYASHITA Tomoyuki  Tokyo Metropolitan Institute of Neuroscience, Department of Life Sciences, Research Fellow (70270668)
WATANABE Chiho  The University of Tokyo, Department of Human Ecology, Professor (70220902)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordssynapse / synapsin I / development / estrogen / estrogen receptor / 細胞・組織 / 神経科学 / プロテオーム / シグナル伝達 / 大脳皮質 / シナプス形成 / プロテオミクス解析 / 培養細胞 / エストロゲン / APC蛋白質 / CaMキナーゼ
Research Abstract

It has become increasingly clear that estrogens, one of steroids hormones, play an important role in learning and memory across the lifespan. Estrogens also play critical roles in the sexual differentiation of the developing rat hypothalamus to form gender-specific neural circuit during about one perinatal week, critically sensitive period to estrogen. The purpose of this research was to investigate the effect of estrogens on neurons, especially on synaptogenesis during development. We performed this research by using cultured hypothalamic and cortical cells and observed estradiol-17 beta (E2)-mediated changes in synaptogenesis. In some studies, we also used endocrine disruptors with estrogenic activities including bisphenol-A, nonylphenol and cadmium (Cd). Treatment of E2 as well as membrane impermeable E2 conjugated to BSA (E2-BSA) for a week significantly increased the punctual area of synapsin I. The increase in punctuate area of synapsin I induced by E2 or E2-BSA was not inhibited by estrogen receptor antagonist, ICI 182,780. Subcellular distribution pattern of synapsin I, diffusely dispersing or punctually clustering, is controlled by multiple protein kinases. Immunoblots showed that E2 suppressed phosphorylation of synapsin I at sites 1, 2 and 3. These sites are known to be phosphorylated mainly by cAMP-dependent protein kinase (PKA) and Ca2+-calmodulin-dependent protein kinase (CaMK) I and II. On the other hand, E2 did not affect phosphorylation at sites 4/5 and 6 which are phosphorylated mainly by mitogen activated protein kinase (MAPK) . These studies and other inhibitor studies suggested that E2-mediated changes of synapsin I am caused through the non- genomic signaling.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (50 results)

All 2008 2007 2006 2005

All Journal Article (38 results) (of which Peer Reviewed: 17 results) Presentation (12 results)

  • [Journal Article] Effects of unoprostone on phosphorylated extracellular signal-regulated kinase expression in endothelin-l-induced retinal and optic nerve damage2008

    • Author(s)
      Munemasa Y., et. al.
    • Journal Title

      Vis Neurosci. 25

      Pages: 197-208

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of post-translational modification of neuronal plasticity-related proteins in hyperalgesia revealed by a proteomic analysis2008

    • Author(s)
      Fujisawa, et. al.
    • Journal Title

      Proteomics 8

      Pages: 1706-1719

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias2008

    • Author(s)
      Yokosuka, et. al.
    • Journal Title

      Toxicol In Vitro 22

      Pages: 1-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of unoprostone on phosphorylated extracellular signal-regulated kinase expression in endothelin-1- induced retinal and optic nerve damage.2008

    • Author(s)
      Munemasa, Y., et. al.
    • Journal Title

      Vis Neurosci 25

      Pages: 197-208

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias.2008

    • Author(s)
      Yokosuka M., et. al.
    • Journal Title

      Toxicol In Vitro 22

      Pages: 1-9

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of post-translational modification of neuronal plasticity-related proteins in hyperalgesia revealed by a proteomic analysis.2008

    • Author(s)
      Fujisawa, et. al.
    • Journal Title

      Proteomics 8

      Pages: 1706-1719

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroprotective effect of 17beta-estradiol against N-methyl-D-aspartate-induced retinal neurotoxicity via p-ERK induction2007

    • Author(s)
      Hayashi Y., et. al.
    • Journal Title

      J.Neurosci Res 85

      Pages: 386-94

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Dorsal root ganglion regulates the transient ERK activation in the dorsal horn of the spinal cord during development2007

    • Author(s)
      Kato. T., et. al.
    • Journal Title

      Neurosci Res 58

      Pages: 402-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Neuroprotective effect of 17beta-estradiol against N-methyl-D-aspartate-induced retinal neurotoxicity via p-ERK induction2007

    • Author(s)
      Hayashi, Y., et. al.
    • Journal Title

      J. Neurosci Res 85

      Pages: 386-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Dorsal root ganglion regulates the transient ERK activation in the dorsal horn of the spinal cord during development.2007

    • Author(s)
      Kato, T., et. al.
    • Journal Title

      Neurosci Res 58

      Pages: 402-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Neuroprotective effect of 17beta-estradiol against N-methyl-D-aspartate-induced retinal neurotoxicity via p-ERK induction.2007

    • Author(s)
      Hayashi Y., et. al.
    • Journal Title

      J. Neurosci Res. 85

      Pages: 386-94

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dorsal root ganglion regulates the transient ERK activation in the dorsal horn of the spinal cord during development.2007

    • Author(s)
      Kato. T., et. al.
    • Journal Title

      Neurosci Res. 58

      Pages: 402-5

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroprotective effect of 17beta-estradiol against N-methyl-D-aspartate-induced retinal neurotoxicity via p-ERK induction.2007

    • Author(s)
      Hayashi Y. et al.
    • Journal Title

      J. Neurosci Res. 85

      Pages: 386-94

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Liver-type fatty acid-binding protein attenuates renal injury induced by unilateral ureteral obstruction2006

    • Author(s)
      Kamijo-Ikemori A., et. al.
    • Journal Title

      Am J Pathol. 169

      Pages: 1107-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pro-apoptotic role of c-Jun in NMDA-induced neurotoxicity in the rat retina2006

    • Author(s)
      Munemasa Y., et. al.
    • Journal Title

      J.Neurosci Res. 83

      Pages: 907-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mechanisms underlying estrogen-induced sexual differentiation in the hypothalamus2006

    • Author(s)
      Ohtani-Kankeko R.
    • Journal Title

      Histol Histopathol 21

      Pages: 317-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Liver-type fatty acid-binding protein attenuates renal injury induced by unilateral ureteral obstruction2006

    • Author(s)
      Kamijo-Ikemori, A., et. al.
    • Journal Title

      Am J Pathol 169

      Pages: 1107-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pro-apoptotic role of c-Jun in NMDA-induced neurotoxicity in the rat retina2006

    • Author(s)
      Munemasa, Y., et. al.
    • Journal Title

      J. Neurosci Res 83

      Pages: 907-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mechanisms underlying estrogen-induced sexual differentiation in the hypothalamus2006

    • Author(s)
      Ohtani-Kankeko, R
    • Journal Title

      Histol Histopathol 21

      Pages: 317-24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Liver-type fatty acid-binding protein attenuates renal injury induced by unilateral ureteral obstruction.2006

    • Author(s)
      Kamijo-Ikemori A. et al.
    • Journal Title

      Am J Pathol. 169

      Pages: 1107-17

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Pro-apoptotic role of c-Jun in NMDA-induced neurotoxicity in the rat retina.2006

    • Author(s)
      Munemasa Y. et al.
    • Journal Title

      J. Neurosci Res. 83

      Pages: 907-18

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mechanisms underlying estrogen-induced sexual differentiation in the hypothalamus.2006

    • Author(s)
      Ohtani-Kankeko R.
    • Journal Title

      Histol Histopathol. 21

      Pages: 317-24

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mechanisms underlying estrogen-induced sexual differentiation in the hypothalamus.2006

    • Author(s)
      Ohtani-Kaneko R.
    • Journal Title

      Histol Histopathol. 21

      Pages: 317-324

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chloride turnover and ion-transporting activities of yolk-sac preparations(yolk balls)separated from Mozambique tilapia embryos and incubated in freshwater and seawater2005

    • Author(s)
      Hiroi J., et. al.
    • Journal Title

      J.Exp.Biol. 208

      Pages: 3851-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Developmental regulation of activated ERK Expression in the Spinal Cord and Dorsal Root Ganglion of the Chick Embryo2005

    • Author(s)
      Kato T., et. al.
    • Journal Title

      Neurosci.Res. 52

      Pages: 11-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia(Oreochromis mossambicus)embryos,by means of triple immunofluorescence staining for Na^+/K^+-ATPase,Na^+/K^+/2Cl^-cotransporter and CFTR anion channel2005

    • Author(s)
      Hiroi J., et. al.
    • Journal Title

      J.Exp.Biol. 208

      Pages: 2023-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Contribution of mitogen-activated protein kinases to NMDA-induced neurotoxicity in the rat retina2005

    • Author(s)
      Munemasa Y., et. al.
    • Journal Title

      Brain Res. 1044

      Pages: 227-40

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential expression of p160 steroid receptor coactivators in the rat testis and enididymis2005

    • Author(s)
      Junko Igarashi-Migitaka J., et. al.
    • Journal Title

      J.Endocrinol. 153

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater2005

    • Author(s)
      Hiroi, J., et. al.
    • Journal Title

      J. Exp. Biol 208

      Pages: 3851-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Developmental Regulation of Activated ERK Expression in the Spinal Cord and Dorsal Root Ganglion of the Chick Embryo2005

    • Author(s)
      Kato, T., et. al.
    • Journal Title

      Neurosci. Res 52

      Pages: 11-19

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2C1- cotransporter and CFTR anion channel2005

    • Author(s)
      Hiroi, J., et. al.
    • Journal Title

      J. Exp. Biol 208

      Pages: 2023-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Contribution of mitogen-activated protein kinases to NMDA-induced neurotoxicitv in the rat retina2005

    • Author(s)
      Munemasa, Y., et. al.
    • Journal Title

      Brain Res 1044

      Pages: 227-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis2005

    • Author(s)
      Junko, Igarashi-Migitaka, J., et. al.
    • Journal Title

      J. Endocrinol 153

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater.2005

    • Author(s)
      Hiroi J. et al.
    • Journal Title

      J Exp Biol, 208

      Pages: 3851-3858

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Developmental Regulation of Activated ERK Expression in the Spinal Cord and Dorsal Root Ganglion of the Chick Embryo.2005

    • Author(s)
      Kato T.et al.
    • Journal Title

      Neurosci.Res. 52

      Pages: 11-19

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na^+/K^+-ATPase, Na^+/K^+/2Cl^- cotransporter and CFTR anion channel.2005

    • Author(s)
      Hiroi J. et al.
    • Journal Title

      J.Exp.Biol. 208

      Pages: 2023-2036

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Contribution of mitogen-activated protein kinases to NMDA-induced neurotoxicity in the rat retina.2005

    • Author(s)
      Munemasa Y. et al.
    • Journal Title

      Brain Res. 1044

      Pages: 227-240

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis.2005

    • Author(s)
      Junko Igarashi-Migitaka J. et al.
    • Journal Title

      J.Endocrinol. 153

      Pages: 1-11

    • Related Report
      2005 Annual Research Report
  • [Presentation] 成熟キンギョ脳におけるc・Fos発現部位の雌雄差2008

    • Author(s)
      大谷 彰子等
    • Organizer
      第60回日本動物学会関東支部会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sexual dimorphism in the expression of c-Fos in the brain of the matured goldfish2008

    • Author(s)
      Ohtani, A., et. al.
    • Organizer
      60th Annual Kanto Branch Meeting of the Zoological Society of Japan
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-03-22
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] エストラジオールによる培養視床下部細胞での熱ショック蛋白質の発現変化-プロテオミクス解析法を用いた検討-2007

    • Author(s)
      大谷-金子 律子, 等
    • Organizer
      第30回日本神経科学大会、第50回日本神経化学会大会、第17回日本神経回路学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteomics analysis of effects of estrogen on developing hypothalamic and cortical cells2007

    • Author(s)
      Ohtani-Kaneko, R., et. al.
    • Organizer
      29th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The effect of estrogen on the developing hypothalamus2007

    • Author(s)
      Ohtani-Kaneko, R
    • Organizer
      11th symposium of Advanced Research Center for Human Sciences, Waseda University
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-01-10
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Increase of heat shock proteins by treatment of cultured hypothalamic cells with 17beta-estradiol.2007

    • Author(s)
      Ohtani-Kaneko, et. al.
    • Organizer
      Neuro 2007
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Proteomics analysis of effects of estrogen on developing hypothalamic and cortical cells2006

    • Author(s)
      R. Ohtani-Kaneko, et. al.
    • Organizer
      The 29th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      京都
    • Year and Date
      2006-07-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteomics analysis of effects of estrogen on developing hypothalamic and cortical cells2006

    • Author(s)
      Ohtani-Kaneko, R., et. al.
    • Organizer
      29th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-07-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 発生過程の視床下部ニューロンに及ぼすエストロゲンの作用2006

    • Author(s)
      大谷-金子 律子
    • Organizer
      早稲田大学人間総合研究所第11回「性と生殖」プロジェクトシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2006-01-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Tumor suppressor adenomatous polyposis coli(APC)はエストロゲン短時間処理によりリン酸化される2005

    • Author(s)
      今野 剛志, 等
    • Organizer
      第28回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2005-07-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Estrogen-induced phosphorylation of tumor suppressor protein APC n cultured neurons2005

    • Author(s)
      Konno, T., et. al.
    • Organizer
      28th Annual Meeting of the Japan Neuroscience Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      2005-07-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Neuroprotective effects of 17beta-estradiol on the rat retina exposed to NMDA administration2005

    • Author(s)
      Y. Hayashi, et. al.
    • Organizer
      The Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale in USA
    • Year and Date
      2005-05-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi