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A peripheral nerve reproduction control structure by one oxidized form galectin-1 instruction macrophage.

Research Project

Project/Area Number 17500265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKanagawa Dental College (2006)
Waseda University (2005)

Principal Investigator

HORIE Hidenori  Kanagawa Dental College, Research Center of Brain and Oral Science, Professor, 歯学部, 教授 (80046135)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroko  Waseda University, School of Advanced Science and Engineering, Associate Professor, 理工学術院, 助教授 (60184769)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsgalectin-1 / macrophage / cytokine / mRNA / LPS / 酸化型ガレクチン-1 / 発生・発達・再生神経科学 / 分子・細胞神経科学
Research Abstract

Oxidized galectin-1 (GAL-1/0x) stimulates macrophages to promote axonal regeneration in peripheral nerves after nerve injury. But, the mechanism of GAL-1/0x, how to regulate macrophages, remains to be clarified. Here we demonstrated effects of GAL-/Ox on mRNA expression of four injury related molecules, interleukin-1β (IL-1β), interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and inducible NO synthase (iNOS) in cultured rat peritoneal macrophages. RT-PCR analysis revealed that IL-6 as well as iNOS mRNA expressions were up-regulated by the treatment with 1 ng/ml GAL-1/0x, but the other mRNA did not show significant differences by the application of GAL-1/0x. Increased IL-6 may promote axonal regeneration in peripheral nerves, but up-regulated iNOS, promoting oxidization, may damage regenerating nerves. This problem was resolved by the following experiments. Using lipopolysaccharide (LPS) is one of the typical substance to stimulate macrophages to promote mRNA expressions of cytoki … More nes and iNOS. When 10 to 100 ng/ml LPS was applied to cultured macrophages, mRNA expressions of IL-1β, IL-6, LIF, and iNOS were up-regulated. The treatment of 1 ng/ml GAL-1/0x reduced their expressions. This inhibition by GAL-1/0x treatment reduced with decreasing its concentration to 0.1 ng/ml and 0.01 ng/ml GAL-1/0x showed no significant inhibitory effect. These results indicate the possibility that GAL-1/0x regulates expressions of IL-1β, IL-6, LIF, and iNOS to a suitable level, otherwise damaging injured tissues in an excess. Since it has been proved that galectin-1 locates as a reduced form in neurons, their axons, and Schwann cells in injured peripheral nerves and is secreted into extra-cellular space to change into oxidized galectin-1 after oxidization, GAL-1/0x may restrict macrophages to secrete the factors in a proper amount resulting with the promotion of recovery. Since GAL-1/0x, macrophage, and LPS are commonly seen in general inflammation, GAL-1/0x is expected to improve inflammation. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (36 results)

All 2006 2005

All Journal Article (36 results)

  • [Journal Article] Adult neurons and Schwann cells in culture as useful tools for the study of diabetic neuropathy.2006

    • Author(s)
      Sango K
    • Journal Title

      Current Diabetes Reviews 2

      Pages: 169-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A carbohydrate binding protein, Galectin-1, promotes proliferation of adult neural stem cell.2006

    • Author(s)
      Sakaguchi M
    • Journal Title

      Proc Natl Sci,USA 103

      Pages: 7112-7117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 成熟動物ニューロン・シュワン細胞の培養系を用いた糖尿病性神経障害の病態解明へのアプローチ2006

    • Author(s)
      三五一憲
    • Journal Title

      末梢神経 : Peripheral Nerve 17

      Pages: 15-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adult neurons and Schwann cells in culture as useful tools for the study of diabetic neuropathy.2006

    • Author(s)
      Sango, K
    • Journal Title

      Current Diabetes Reviews 2

      Pages: 169-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A carbohydrate binding protein, Galectin-1, promotes proliferation of adult neural stem cell.2006

    • Author(s)
      Sakaguchi M
    • Journal Title

      Proc Natl Sci, USA 103

      Pages: 7112-7117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An approach to the pathogenesis of diabetic neuropathy with cultured adult animal neurons and Schwann cells.2006

    • Author(s)
      Sango K
    • Journal Title

      Peripheral Nerve 17

      Pages: 15-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A carbohydrate binding protein, Galection-l,promotes proliferation of adult neural stem cell.2006

    • Author(s)
      Sakaguchi M, 他8名, Horie H, 他5名, Okano H.
    • Journal Title

      Proc Natl Sci, USA 103

      Pages: 7112-7117

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 成熟動物ニュ-ロン・シュワン細胞の培養系を用いた糖尿病性神経障害の病態解明へのアプロ-チ2006

    • Author(s)
      三五 一憲, 斎藤 春洋, 堀江 秀典
    • Journal Title

      末梢神経 : Peripheral Nerve 17

      Pages: 15-22

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Selective induction of ΔFosB in the brain after transient forebrain ischemia accompanied by an increased expression of galectin-1, and the implication of ΔFosB and galectin-1 in neuroprotection and neurogenesis.2005

    • Author(s)
      Kurushima H
    • Journal Title

      Cell Death Differ. 12

      Pages: 1078-1096

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidized Galectin-1 Advances the Myelination of Regenerating Fibers and the Functional Recovery after Peripheral Nerve Injury.2005

    • Author(s)
      Kadoya T
    • Journal Title

      Neurosci Lett. 380

      Pages: 284-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of galecin-1 expression by axotomy in rat primary afferent neurons.2005

    • Author(s)
      McGraw J
    • Journal Title

      Exp Neurol. 195

      Pages: 103-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Galectin-1 as an Essential Factor in Nervous System.2005

    • Author(s)
      Horie H
    • Journal Title

      Curr Drug Targets 6

      Pages: 373-374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural and Functional Studies of Galectin-1 : A Novel Axonal Regeneration-Promoting Activity for Oxidized Galectin-12005

    • Author(s)
      Kadoya T
    • Journal Title

      Curr Drug Targets 6

      Pages: 375-383

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidized galectin-1 is an essential factor for peripheral nerve regeneration.2005

    • Author(s)
      Horie H
    • Journal Title

      Curr Drug Targets 6

      Pages: 385-394

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of the neuronal cell fate by ΔFosB and its downstream target, galectin-1.2005

    • Author(s)
      Miura T
    • Journal Title

      Curr Drug Targets 6

      Pages: 437-448

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Impaired neurite regeneration in the retina of a murine model of Sandhoff disease.2005

    • Author(s)
      Sango K
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 3420-3425

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Intravitreal macrophage activation enables cat retinal ganglion cells to regenerate injured axons into the mature optic nerve.2005

    • Author(s)
      Okada T
    • Journal Title

      Exp Neurol 196

      Pages: 153-163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 末梢神経再生を誘導する酸化型ガレクチンー1. 特集「末梢神経系と脊髄の再生メカニズム」2005

    • Author(s)
      堀江秀典
    • Journal Title

      細胞 37

      Pages: 10-13

    • NAID

      40006792228

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 末梢神経系におけるガレクチンー1の発現分布と生理機能2005

    • Author(s)
      三五一憲
    • Journal Title

      末梢神経 : Peripheral Nerve 16

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Selective induction of ΔFosB in the brain after transient forebrain ischemia accompanied by an increased expression of galectin-1, and the implication of AFosB and galectin-1 in neuroprotection and neurogenesis.2005

    • Author(s)
      Kurushima H, Y.
    • Journal Title

      Cell Death Differ. 12

      Pages: 1078-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidized Galectin-1 Advances the Myelination of Regenerating Fibers and the Functional Recovery after Peripheral Nerve Injury.2005

    • Author(s)
      Kadoya T
    • Journal Title

      Nerve Injury. Neurosci. Lett. 380

      Pages: 284-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Editorial "Galectin-1 as an Essential Factor in Nervous System"2005

    • Author(s)
      Horie H
    • Journal Title

      Curr Drug Targets 6

      Pages: 373-374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Regulation of the neuronal cell fate by FosB and its downstream target, galectin-1.2005

    • Author(s)
      Miura T
    • Journal Title

      Curr Drug Targets 6

      Pages: 437-448

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidized Galectin-1 Initiates periphral nerve Regeneration.2005

    • Author(s)
      Horie H
    • Journal Title

      Cell 37

      Pages: 10-13

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histochemical localization and possible functions of galecin-1 in the peripheral nervous system.2005

    • Author(s)
      Sango K
    • Journal Title

      Peripheral Nerve 16

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Selective induction of delta-FosB in the brain after transient forebrain ischemia accompanied by an increased expression of galectin-1, and2005

    • Author(s)
      Kurushima H, 他3名, Horie H, 他5名, Nakabeppu Y.
    • Journal Title

      Cell Death Differ 12

      Pages: 1078-1096

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Oxidized Galectin-1 Advances the Myelination of Regenerating Fibers and the Functional Recovery after Peripheral Nerve Injury2005

    • Author(s)
      Kadoya T, Oyanagi, K, 他5名, Horie H.
    • Journal Title

      Neurosci.Lett 380

      Pages: 103-114

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of galecin-1 expression by axotomy in rat primary afferent neurons2005

    • Author(s)
      McGraw J, 他3名, Horie H, 他2名, Ramer MS.
    • Journal Title

      Exp Neurol. 195

      Pages: 103-114

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Editorial "Galectin-1 as an Essential Factor in Nervous System2005

    • Author(s)
      Horie H.
    • Journal Title

      Curr Drug Targets, 6

      Pages: 373-374

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structural and Functional Studies of Galectin-1 : A Novel Axonal Regeneration-Promoting Activity for Oxidized Galectin-12005

    • Author(s)
      Kadoya T, Horie H
    • Journal Title

      Curr Drug Targets 6

      Pages: 375-383

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Oxidized galectin-1 is an essential factor for peripheral nerve regeneration.2005

    • Author(s)
      Horie H, Kadoya T
    • Journal Title

      Curr Drug Targets 6

      Pages: 385-394

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of the neuronal cell fate by DFosB and its downstream target, galectin-12005

    • Author(s)
      Miura T, Ohnishi Y, Kurushima H, Horie H, Kadoya T, Nakabeppu Y
    • Journal Title

      Curr Drug Targets 6

      Pages: 437-448

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Impaired neurite regneration in the retina of a murine model of Sandhoff disease2005

    • Author(s)
      Sango K, Takano M, Ajiki K, 他3名, Horie H, Yamanaka S
    • Journal Title

      Invest Ophthalmol Vis Sci 46

      Pages: 3420-3425

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Axonal regeneration of cat retinal ganglion cells in the crusted optic nerve is promoted with activation of macrophages2005

    • Author(s)
      Okada T, Ichikawa M, Tokita Y, Horie H, 他2名, Watanabe M
    • Journal Title

      Exp Neurol 196

      Pages: 153-163

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 末梢神経再生を誘導する酸化型ガレクチン-12005

    • Author(s)
      堀江秀典
    • Journal Title

      細胞 37

      Pages: 10-13

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 末梢神経系におけるガレクチン-1の発現分布と生理機能2005

    • Author(s)
      三五一憲, 堀江秀典, 門屋利彦
    • Journal Title

      末梢神経:Peripheral Nerve 16

      Pages: 1-7

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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