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Developmental of the brainstem NTS function studied by optical imaging and living stain methods

Research Project

Project/Area Number 17500272
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SATO Katsushige  Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (80291342)

Co-Investigator(Kenkyū-buntansha) SATO Yoko (MOMOSE Yoko)  Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院医歯学総合研究科, 講師 (70251501)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsoptical recording / voltage-sensitive dye / central nervous system / functiogenesis / rat embryo / chick embryo / NTS / living stain
Research Abstract

Multiple-site optical recording with a fast voltage-sensitive dye, absorption dye NK2761, was used to study the developmental organization of functional synaptic networks in the vagal pathway. Glutamatergic excitatory postsynaptic potentials (EPSPs) evoked by vagus nerve stimulation was first detected from the nucleus of the tractus solitarius (NTS) at embryonic day 7 (E7) in chick embryos and E15 in rat embryos, when morphological differentiation of pre- and postsynaptic neurons is incomplete. When extracellular Mg^<2+> removed, small EPSPs were elicited at E6 in chick embryos and E14 in rat embryos. These results suggest that synaptic function mediated by N-methyl-D-aspartate (NMDA) receptors is latently generated one day before the expression of glutamatergic EPSP. Functional synapses related to the glossophyaryngeal nerve appear to be generated at the same time as the vagus nerve, but their spatial distribution was different from that of the vagus nerve. We further investigated the development of second synaptic pathways from the NTS to higher centers, and found that neuronal circuits from the NTS are already generated when the primary afferents form functional synapses with NTS neurons.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (37 results)

All 2007 2006 2005 Other

All Journal Article (37 results)

  • [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., et al.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      European Journal of Neuroscience (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki, T. et al.
    • Journal Title

      Brain Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GABA-induced intracellular Ca^<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., Kinoshita, M, Sato, K.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical mapping of spatiotemporal emergence of functional synaptic connections in the embryonic chick olfactory pathway.2007

    • Author(s)
      Sato, K., Kinoshita, M., Momose-Sato, Y.
    • Journal Title

      Neuroscience 144

      Pages: 1334-1346

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] GABA-induced intracellular Ca~<2+> elevation in the embryonic chick brainstem slice.2007

    • Author(s)
      Momose-Sato, Y., et al.
    • Journal Title

      Neuroscience Letters 411

      Pages: 42-46

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.2007

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      European Journal Neuroscience (,in press.)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.2007

    • Author(s)
      Sasaki, T. et al.
    • Journal Title

      Brain Research (,in press.)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1949-1962

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission.2006

    • Author(s)
      Ren, J., et al.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Optical recording of vagal pathway formation in the embryonic brainstem.2006

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical 126-127

      Pages: 39-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F. et al.
    • Journal Title

      BMC Neuroscience 7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission.2006

    • Author(s)
      Ren, J., Momose-Sato, Y., Sato, K., Greer, J.J.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y., Glover, J.C., Sato, K.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1946-1962

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F., Komine, O., Sato, K., Kanamatsu, T., Uchimura, M., Tanaka, K., Ohki-Hamazaki, H.
    • Journal Title

      BMC Neuroscience 7

      Pages: 75-87

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of functional synaptic connections in the auditory system visualized with optical recording : Afferent-evoked activity is present from early stages.2006

    • Author(s)
      Momose-Sato, Y., et al.
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1949-1962

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imprinting modulates processing of visual information in the visual wulst of chicks.2006

    • Author(s)
      Maekawa, F., et al.
    • Journal Title

      BMC Neuroscience 7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rhythmic neuronal discharge in the medulla and spinal cord of fetal rats in the absence of synaptic transmission2006

    • Author(s)
      Ren, J.et al.
    • Journal Title

      Journal of Neurophysiology 95

      Pages: 527-534

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Optical recording of vagal pathway formation in the embryonic brainstem2006

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging.2005

    • Author(s)
      Sato, K., et al.
    • Journal Title

      NeuroImage 25

      Pages: 1292-1301

    • NAID

      130007039125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS.2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 94

      Pages: 1606-1622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery.2005

    • Author(s)
      Nariai, T. et al.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe.2005

    • Author(s)
      Wang, S. et al.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording.2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Membrane Biology 208

      Pages: 183-191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging.2005

    • Author(s)
      Sato, K., Nariai, T., Tanaka, Y., Maehara, T., Miyakawa, N., Sasaki, S., Momose-Sato, Y., Ohno, K.
    • Journal Title

      NeuroImage 25

      Pages: 1292-1301

    • NAID

      130007039125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS.2005

    • Author(s)
      Momose-Sato, Y., Honda, Y., Sasaki, H., Sato, K.
    • Journal Title

      Journal of Nerophysiology 94

      Pages: 1606-1622

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery.2005

    • Author(s)
      Nariai, T., Sato, T., Hirakawa, K., Ohta, Y., Tanaka, Y., Ishiwata, K., Ishii, K., Kamino, K., Ohno, K.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe.2005

    • Author(s)
      Wang, S., Zhang, S., Sato, K., Srinivasan, M.V.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording.2005

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Journal of Membrane Biology 208

      Pages: 183-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional representation of the finger and face in the human somatosensory cortex : Intraoperative intrinsic optical imaging2005

    • Author(s)
      Sato, K. et al.
    • Journal Title

      NeuroImage 25

      Pages: 1292-1301

    • NAID

      130007039125

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Optical imaging of large-scale correlated wave activity in the developing rat CNS2005

    • Author(s)
      Momose-Sato, Y. et al.
    • Journal Title

      Journal of Neurophysiology 94

      Pages: 1606-1622

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Visualization of somatotopic representation of sensory cortex with intrinsic optical signals as guides for brain tumor surgery2005

    • Author(s)
      Nariai, T. et al.
    • Journal Title

      Journal of Neurosurgery 103

      Pages: 414-423

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Maturation of odor representation in the honeybee antennal lobe2005

    • Author(s)
      Wang, S. et al.
    • Journal Title

      Journal of Insect Physiology 51

      Pages: 1244-1254

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Primary vagal projection to the contralateral non-NTS region in the embryonic chick brainstem revealed by optical recording2005

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Journal of Membrane Biology 208(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Spontaneous depolarization waves of multiple origins in the embryonic rat CNS.

    • Author(s)
      Momose-Sato, Y., Kinoshita, M., Sato, K.
    • Journal Title

      European Journal of Neuroscience (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of bioradiography in human brain slices and preoperative PET imaging.

    • Author(s)
      Sasaki, T., Nariai, T., Maehara, T., Sato, K., Oda, K., Ishii K.
    • Journal Title

      Brain Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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