The efficient of method for chimara mice.

• Project/Area Number: 17500292
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokyo Medical University
• Principal Investigator: SUDO Ktsuko Tokyo-medical University, medical, Lecturer, 医学部, 講師 (50126091)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ES cell / mouse chimaeras / germline / rate of chimaera / aggregation / cell cycle / KO mice / 遺伝子改変動物

Research Abstract

The difficulties in introducing ES cell derived genomes into the mouse germ line. And more difficulties has germ line chimaerism.
And then we are improvement of aggregation methods.
We have many KO mice(IL-1α,IL-1β,IL-1Ra, IL-16,IL-17,MD-1, ADAM12,NR2.....).
But very low percentage of germ line chimera that cell injection into blastocyst and 8 cell stage aggregation.
Very important is establishment of good embryonic stem cell line.
2end important is ES cell cycle and embryo stage.
We had try on several medicines induced same stage cell cycle.

Research Products

• [Journal Article] Abnormal T cell activation caused by the imbalance of the IL-1/IL-1R Antagonist system is responsible for the development of experimental autoimmune encephalomyelitis. (2006)
  • Author(s): Matsuki, T., Nakae, S., Sudo, K., Horai, R., Iwakura, Y.
  • Journal Title: Int. Immunology. 18 Pages: 399-407
  • NAID: 10020621858
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genetic Dissection of the Effects of Stimulatory and Inhibitory IgG Fc Receptors on Murine Lupus. (2006)
  • Author(s): Lin, Q., Xiu, Y., Jiang, Y., Tsurui, H., Nakamura, K., Kodera, S., Ohtuji, M., Ohtsuji, N., Shiroiwa, W., Tsukamoto, K., Amano, H., Amano, E., Kinoshita, K., Sudo, K., Nishimura, H., Izui, S., Shirai, T., Hirose, S.
  • Journal Title: Journal of Immunology Pages: 1646-1654
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] IL-17 Plays an Important Role in the Development of Experimental Autoimmune Encephalomyelitis. (2006)
  • Author(s): Komiyama, Y., Nakae, S., Matsuki, T., Namubu, A., Ishigame, H., Kakuta, S., Sudo, K., Iwakura, Y.
  • Journal Title: Jouenal of Immunology Pages: 566-573
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] NR2B tyrosine phosphorylation modulates fear learning as well as amygdaloid synaptic plasticity (2006)
  • Author(s): Nakazawa, T., Komai, S., Watanabe, A., Kiyama, Y., Fukaya, M., Arima-Yoshida, F., Horai, R., Sudo, K., Ebine, K., Delawary, M., Goto, J., Umemori, H., Tezuka, T., Iwakura, Y., Watanabe, M., Yamamoto, T., Manabe, T.
  • Journal Title: EMBO Journal 25 Pages: 2867-2877
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Abnormal T cell activation caused by the imbalance of the IL-1/IL-1RAntagonist system is responsible for the development of experimental autoimmune encephalomyelitis.. (2006)
  • Author(s): Matsuki, T., Nakae, S., Sudo, K., Horai, R., Iwakura, Y
  • Journal Title: Int.Immunology. 18 Pages: 399-407
  • NAID: 10020621858
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genetic Dissection of the Effects of Stimulatory and Inhibitory IgG Fc Receptors on Murine Lupus. (2006)
  • Author(s): Lin, Q., Xiu, Y., Jiang, Y., Tsurui, H., Nakamura, K., Kodera, S., Ohtuji, M., Ohtsuji, N., Shiroiwa, W., Tsukamoto, K., Amano, H.Amano, E., Kinoshita, K., Sudo, K., Nishimura, H., Izui, S., Shirai, T., Hirose, S.
  • Journal Title: Journal of Immunology Pages: 1646-1654
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] IL-17 Plays an Important Role in the Development of Experimental Autoimmune Encephalomyelitis. (2006)
  • Author(s): omiyama, Y., Nakae, S., Matsuki, T., Namubu, A., Ishigame, H., Kakuta, S., Sudo, K., Iwakura, Y
  • Journal Title: Journal of Immunology Pages: 566-573
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] NR2B tyrosine phosphorylation modulates fear learning as well as amygdaloid synaptic plasticity (2006)
  • Author(s): Nakazawa, T., Komai, S., Watanabe, A., Kiyama, Y., Fukaya, M., Arima-Yoshida, F., Horai, R., Sudo, K., Ebine, K., Delawary, M., Goto, J., Umemori, H., Tezuka, T., Iwakura, Y., Watanabe, M., Yamamoto, T., Manabe.T.
  • Journal Title: EMBO Journal 25 Pages: 2867-2877
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Abnormal T cell activation caused by the imbalance of the IL-1/IL-1R Antagonist system is responsible for the development of experimental autoimmune encephalomyelitis. (2006)
  • Author(s): Matsuki, T., Nakae, S., Sudo, K., Horai, R., Iwakura,Y.
  • Journal Title: Int. Immunology. 18 Pages: 399-407
  • NAID: 10020621858
• [Journal Article] Genetic Dissection of the Effects of Stimulatory and Inhibitory IgG Fc Receptors on Murine Lupus. (2006)
  • Author(s): Lin, Q., Xiu, Y., Jiang, Y., Tsurui, H., Nakamura, K., Kodera, S., Ohtuji, M., Ohtsuji, N., Shiroiwa, W., Tsukamoto, K., Amano, H., Amano, E., Kinoshita, K., Sudo, K., Nishimura, H., Izui, S., Shirai,T., Hirose,S.
  • Journal Title: Journal of Immunology Pages: 1646-1654
• [Journal Article] IL-17 Plays an Important Role in the Development of Experimental Autoimmune Encephalomyelitis. (2006)
  • Author(s): Komiyama, Y., Nakae, S., Matsuki, T., Namubu, A., Ishigame, H., Kakuta, S., Sudo, K., Iwakura,Y.
  • Journal Title: Jouenal of Immunology Pages: 566-573
• [Journal Article] NR2B tyrosine phosphorylation modulates fear learning as well as amygdaloid synaptic plasticity (2006)
  • Author(s): Nakazawa, T., Komai, S., Watanabe, A., Kiyama, Y., Fukaya, M., Arima-Yoshida, F, Horai, R., Sudo, K., Ebine, K., Delawary, M., Goto, J., Umemori, H., Tezuka, T., Iwakura, Y., Watanabe, M., Yamamoto,T., Manabe,T.
  • Journal Title: EMBO Journal 25 Pages: 2867-2877
• [Journal Article] Mice Deficient in the Rab5 Guanine Nucleotide Exchange Factor ALS2/alsin Exhibit Age-Dependent Neurological Deficits and Altered Endosome Traffiking (2006)
  • Author(s): Hadano, S., Genn, SC., Kakuta, S., Otomo, A., Sudo, K.
  • Journal Title: Hum.Mol.Genet. 15 Pages: 233-250
• [Journal Article] Abnomal T cell activation caused by the imbalance of the IL-1/IL-1R antagonist system is responsible for the development experimental autoimmune encephalomyelitis. (2006)
  • Author(s): Matsuki, T., Nakae, S., Sudo, K., Horai, R., Iwakura, Y.
  • Journal Title: Int.Immunol. 18 Pages: 399-407
• [Journal Article] Aberrant Development of Mammary Glands, but Precocious Expression of β-casein in Transgenic Females Ubiquitously Expressing Whey Acidic Protein Transgene (2005)
  • Author(s): Iwamori, T., Oosawa, M., Nukumi, N., Kano, K., Sudo, K., Naito, K., Tojo, H.
  • Journal Title: J.of Reproduction and Development 51 Pages: 579-592
  • NAID: 10027325866