| Project/Area Number |
17500319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Toin University of Yokohama |
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Principal Investigator |
YOH Kodera Toin University of Yokohama, Department of Life and Environmenatal Science, Associate Professor (80205426)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Hiroyuki Toin University of Yokohama, Department of Life and Environmenatal Science, Professor (60189313)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,350,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Enzyme / Biomaterial / Protein / Chemical modification / Metabolism / Clearance time / タンパク質の化学修飾 / ポリエチレングリコール / スーパーオキシドジスムターゼ / コラーゲン関節炎 |
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| Research Abstract |
The target of this project is, 1) Chemical modification of protein drugs with a series of synthetic macromolecule, polyethylene gycol, 2) and to change their original characteristics, such as prolongation of blood circulation life-times, reduction of immune response, inducing immune tolerance, and increasing biological affinity of synthetic or allogenic protein drugs.
An anti-inflammatory enzyme, manganese-type superoxide dismutase was coupled with single-chained and double-chained polyethylene glycol derivatives (PEG). Especially, a branched PEG derivative with cyanuric acid as a spacer seems to be a best choice for prolongation of blood circulating life-time. Inflammatory activity of PEG-superoxide dismutase was checked in collagen-induced arthritis as well as reumatoid arthritis using DAB/1 and SKG mice, respectively.
Phenylalanine ammonia-lyase was also coupled with PEG derivatives, but another type of branced PEG derivatives was needed due to the enzymic stability. We have to synthesize various PEG derivatives with varied spacers.
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