| Project/Area Number |
17500480
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
KUROSAWA Hisashi Juntendo University, School of Medicine, Professor, 医学部, 教授 (50010301)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Naoki Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (50345013)
KIM Sung-gon Juntendo University, School of Medicine, Lecturer, 医学部, 助手 (70255694)
OHSAWA Aki Juntendo University, School of Medicine, Lecturer, 医学部, 助手 (50407251)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2006: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | knee osteoarethritis / home-base exercise / SNP / joint space width / MMP-1 / 運動療法 / 有効性 / 自然経過 |
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| Research Abstract |
Medical records and DNA samples were obtained from 302 knee-OA patients who began the exercise and from 115 non-OA subjects. The extent of OA severity was assessed based on tibiofemoral joint space width (JSW) in the medial compartment. Three MMP-1 genotypes were determined (rs17886048(1GT/2G), rs470221, and rs2071230).
Subjects with both 1G1G (rs17886048, 1G/2G) and GG (rs470221) genotypes showed significant protection against knee OA (OR 0.40; p=0.03), whereas those with both 2G2G and GA+AA (rs470221) SNPs were at significant risk for knee OA (OR 3.04; p=0.02). OA patients with a combination of genotypes GG (rs470221) and AA (rs2071230) had less JSW than those with other combinations (p=0.0006). Moreover, the subjects with this genotype were more sensitive to deteriorating effect of being overweight and showed a significantly more severe phenotype when they had BMI over 27 compared to other subjects (p=0.01).
This study suggested that MMP-1 SNPs are associated with susceptibility to knee OA and with the severity of condition. Therefore, these SNPs may be useful predictive and prognostic markers for knee OA, and a molecular understanding of the function of the SNPs may help to clarify the role(s) of MMP-1 in cartilage destruction.
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