| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
Isoflavones, such as genistein and daidzein, are phytoestrogens present in soybean foods and have estrogenic activity. In this study, we examined the combined estrogenic effect of soybean extract (Nijiru) containing human intake dose level of isoflavones (10 mg/kg/day) as soybean food and endocrine disruptor.
Soybeans were boiled for 40 min at 120"C in water, and Nijiru was produced and was then freeze-dried to make a powder. Nijiru powder contained daidzin, glycitin, genistin, daidzein, glycitein, and genistein. Nijiru (1900-6600 mg/kg corresponding to dose of total isoflavone 10 mg/kg) was administered to ovariectomized ICR mice by oral gavage for 11 consecutive days. The synthetic endocrine-active hormone diethylstilbestrol (DES) (0.05 mg/kg) was then co-administered orally with Nijiru and continued for another 3 days. The relative uterine weight (% of body weight) of Nijiru+DES treatment was slightly higher than that of DES alone. Similarly, synthetic endocrine-active compound bisphenol A (BPA) (300 mg/kg) or pesticide methoxychlor (MX) (50 mg/kg) were co-administered orally with Nijiru and continued for another 3 days. Although Nijiru+MX treatment increased (vs. MX alone) the relative uterine weight, Nijiru+BPA treatment did not increase (vs. BPA alone) the relative uterine weight.
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