|Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Polyphenolics came from various edible plants can be useful for the cancer prevention. Antitumor-promoting activities of polyphenolic extract prepared from heated-or unheated-various foodstuffs, were evaluated as the indicator of food function. The purpose of this study is proposing the guideline which can limit the loss of antitumor activities by various heat-cooking, for our healthy life.
Preparation of polyphenolic extracts: For the purpose of this study, it is needed to obtain the much more amount of polyphenolic extract without wounded. As various examinations, we established the preparation procedure of polyphenolic fractions, which included the pulverization of edible plants using liquid-nitrogen, methanol extraction, and cleanup of polyphenolics using Sephadex LH-20 column chromatography. To determine the amount of polyphenol components, it is used the Folin-Denis method and HPLC and/or HPLC-MS/MS analysis. Antitumor-promoting effects were estimated using the JB6 cell neoplastic transformation induced by tetradecanoyl phorbol ester (TPA).
Results and Discussions In this study, it was used 12 kinds of edible plants of ginkgo nut, burdock, perilla leaf, Crythanthemum coronarium, soybean, onion, eggplant, broccoli, spinach, lotus root, apple, raisin, which were heated or unheated on microwave oven. To compare the unheated-foodstuffs, the amounts of polyphenolic components and inhibitory effect of TPA-promotion (IC_<50>) showed decrease, increase, and unchanged were observed in heated-ones. Those are indicated that we need to more carefully prepare the foods to limit the loss of food-functions. We will propose the guideline for the art of cooking to hold the minimum loss of cancer preventive food function in the near future.