| Project/Area Number |
17510041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Chiba University |
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Principal Investigator |
FUKATA Hideki Chiba University, Graduate School of Medicine, Department of Bioenvironmental Medicine, Associate Professor, 大学院医学研究院, 特任助教授 (00359598)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Chisato Chiba University, Graduate School of Medicine, Department of Bioenvironmental Medicine, Professor, 大学院医学研究院, 教授 (90174375)
KOMIYAMA Masatoshi Chiba University, Center Environment, Health and Field Science, Assistant Professor, 環境健康都市園芸フィールド科学教育研究センター, 講師 (70175339)
MATSUNO Yoshiharu Chiba University, Graduate School of Medicine, Department of Bioenvironmental Medicine, Research Associate, 大学院医学研究院, 助手 (00376378)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | mouse / DNA methylation / RLGS / MCA-RDA |
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| Research Abstract |
Fetal and neonatal exposure to diethylstilbestrol (DES) is known to cause many abnormalities, such as cancer, in the male and female reproductive tracts later in life, and epigenetic mechanisms, such as DNA methylation, may be involved in these processes. In the present study, newborn C57BL/6 mice were exposed to 0, 0.003, 0.3, 3 μg of DES from postnatal days 1 to 5. Subsequently, restriction landmark genomic scanning (RLGS), which can analyze genome-wide DNA methylation, was performed to clarify whether or not aberrant DNA methylation was present in the epididymis and uterus of the DNA methylation, was performed to clarify whether or not aberrant DNA methylation was present in the epididymis or the uterus of the DES-treated mice at day 30. The RLGS analysis revealed that 7 loci of the genomic DNA were demethylated and 1 locus was methylated in the epididymis and 5 loci of the genomic DNA were demethylated and 6 loci were methylated in the uterus of the DES-treated mice. Four of these loci in the epididymis and 3 of these loci in uterus specifically demethylated in DES-treated mice were cloned, DES-induced abnormalities of reproductive organs are associated with altered expression levels of DNA methylation. As these data suggest that chemical exposure is responsible for epigenetic alteration, we insist the necessity of the new risk assessment focusing on epigenetic alteration.
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