| Project/Area Number |
17510044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Okayama University |
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Principal Investigator |
NEGISHI Tomoe Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Division of Pharmaceutical Sciences, Associate Professor (80116491)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Kazuaki University of Occupational and Environmental Health, Institute of Industrial Ecological Sciences, Associate Professor (60161262)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Oxidative damage / Environmental cigarette smoke / Drosophila / 8-Hydroxydeoxiquanosine / Uric acid / Reproducibility / 酸化傷害 / 体細胞突然変異 |
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| Research Abstract |
Epidemiological evidence indicates that cigarette smoke is harmful for our health. Many investigations have documented damage induced by mainstream cigarette smoke. We examined the biological effects induced by environmental cigarette smoke (ECS) using Drosophila. When 3rd instar larvae were exposed to ECS, the eclosion-rate of a urate-null mutant strain decreased significantly compared to that of a wild strain. As urate is considered to function as an antioxidant in Drosophila, cigarette smoke appears to induce oxidative damage in larvae. To estimate the effect of ECS on offspring development, we observed the viability of flies emerging from eggs that were produced by matings between males and females eclosed from larvae exposed or not exposed to ECS. Pairs of the urate-null strain in which both males and females were exposed showed the lowest viability. These results suggest that third instar larvae were damaged by ECS affecting not only their own viability but also that of the next generation.
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