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Development and application of the rapid cultivation and isolation method of petroleum-degrading bacteria by enrichment culture using soils

Research Project

Project/Area Number 17510061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionHokkaido University

Principal Investigator

OKUYAMA Hidetoshi  Hokkaido University, Faculty of Environmental Earth Science, associate Professor, 大学院地球環境科学研究院, 助教授 (90125295)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsPetroleum-degrading bacteria / Soil DNA / Naphthene degrader / Isoalkane degrader / Crude oil-degrading bacteria / Autochthonous bioaugmentation / Soil cultivation
Research Abstract

1.The bioaugmentation method (BA) is a useful technology for decontamination of petroleum-contaminated soils. For this purpose it is necessary to isolate petroleum-degrading bacteria with high activity and to apply such bacteria to soils. To promptly respond to oil spills from oil tankers and tanks isolation and characterization of oil-degrading bacteria should be also prompt. In this study I aimed to develop prompt isolation procedures of petroleum-degrading bacteria.
2.Isolation of petroleum-degrading bacteria from soils (petroleum-contaminated and petroleum-non-contaminated soils) was achieved by cultivating them with soil extracts.
3.Petroleum-degrading isolates from soils are generally able to efficiently degrade petroleum under liquid cultivation conditions. However, most of them did not exhibit significant degrading activity of petroleum in soils.
4.One strain from petroleum-non-contaminated paddy soils was isolated.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006

All Journal Article (12 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Isolation and characterization of bacteria from soil contaminated with diesel oil, and the possible use of these in autochthonous bioaugmentation.2007

    • Author(s)
      A.Ueno, Y.Ito, I.Yumoto, H.Okuyama
    • Journal Title

      World J. Microbiol. Biotechnol. (In press)

    • NAID

      120000955815

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Degradation of very long chain n-alkanes (C_36 and C_40) by Pseudomonas aeruginosa WatG2007

    • Author(s)
      M.Hasanuzzaman, A.Ueno, H.Ito, Y.Ito, Y.Yamamoto, I.Yumoto, H.Okuyama
    • Journal Title

      Int. Biodeterior. Biodegradation 59・1

      Pages: 40-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of bacteria from soil contaminated with diesel oil, and the possible use of these in autochthonous bioaugmentation.2007

    • Author(s)
      Akio Ueno, Yukiya Ito, Isao Yumoto, Hidetoshi Okuyama
    • Journal Title

      World J. Microbiol. Biotechnol (In press)

    • NAID

      120000955815

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Degradation of very long chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa WatG.2007

    • Author(s)
      M.Hasanuzzaman, A.Ueno, H.Ito, Y.Ito, Y.Yamamoto, I.Yumoto, H.Okuyama
    • Journal Title

      Int. Biodeterior. Biodegradation 59(1)

      Pages: 40-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Degradation of very long chain n-alkanes (C_<36> and C_<40>) by Pseudomonas aeruginosa WatG2007

    • Author(s)
      M.Hasanuzzaman, A.Ueno, H.Ito, Y.Ito, Y.Yamamoto, I.Yumoto, H.Okuyama
    • Journal Title

      Int. Biodeterior. Biodegradation 59・1

      Pages: 40-43

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bacterial community change analysis of diesel oil-contaminated soil microcosms biostimulated with Luria-Bertani medium and bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG.2006

    • Author(s)
      A.Ueno, Y.Ito, Y.Yamamoto, I.Yumoto, H.Okuyama
    • Journal Title

      J. Basic Microbiol. 46・4

      Pages: 310-317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Verification of degradation of diesel oil by Pseudomonas aeruginosa strain WatG in soil microcosms.2006

    • Author(s)
      A.Ueno, M.Hasanuzzaman, I.Yumoto, H.Okuyama
    • Journal Title

      Curr. Microbiol. 52・3

      Pages: 2006-2006

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bacterial community change analysis of diesel oil-contaminated soil microcosms biostimulated with Luria-Bertani medium and bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG.2006

    • Author(s)
      A.Ueno, Y.Ito, Y.Yamamoto, I.Yumoto, H.Okuyama
    • Journal Title

      J. Basic Microbiol. 46 (3)

      Pages: 310-317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Verification of degradation of diesel oil by Pseudomonas aeruginosa strain WatG in soil microcosms.2006

    • Author(s)
      A.Ueno, M.Hasanuzzaman, I.Yumoto, H.Okuyama
    • Journal Title

      Curr. Microbiol. 52(3)

      Pages: 182-185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bacterial community change analysis of diesel oil-contaminated soil microcosms biostimulated with Luria-Bertani medium and bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG.2006

    • Author(s)
      Akio Ueno, Yukiya Ito, Yusuke Yamamoto, Isao Yumoto, Hidetoshi Okuyama
    • Journal Title

      J. Basic Microbiol. 46・4

      Pages: 310-317

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bacterial community change analysis of diesel oil-contaminated soil microcosms biostimulated with Luria-Bertani medium and bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa stain WatG.2006

    • Author(s)
      Akio Ueno, Yukiya Ito, Yusuke Yamamoto, Isao Yumoto, Hidetoshi Okuyama
    • Journal Title

      J. Basic Microbiol. (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Verification of degradation of diesel oil by Pseudomonas aeruginosa strain WatG in soil microcosms.2006

    • Author(s)
      A Ueno, M.Hasanuzzaman, I.Yumoto, H.Okuyama
    • Journal Title

      Curr. Microbiol. 52・3

      Pages: 182-185

    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 核酸調製方法および核酸調製キット2006

    • Inventor(s)
      奥山英登志, 上野晃生, 寺垣純
    • Industrial Property Rights Holder
      国立大学法人北海道大学
    • Industrial Property Number
      2006-053564
    • Filing Date
      2006-02-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 核酸調製方法および核酸調整キット2006

    • Inventor(s)
      奥山英登志, 上野弘文晃生, 寺垣純
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2006-053564
    • Filing Date
      2006-02-28
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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