Development of system to recover heavy metal from ore, polluted soil and wastewater by using iron-oxidizing bacterium
| Project/Area Number |
17510069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Okayama University |
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Principal Investigator |
TAKEUCHI Fumiaki Okayama University, Health and Environment Center, Associate Professor (90294446)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIO Tsuyoshi OKAYAMA UNIVERSITY, Graduate School of Natural Science and Technology, Technology Professor (20033269)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,040,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | iron oxidizing bacterium / Acidithiohacillus ferrooxidans / Bioleaching / Rioremediation / Mercury-Resistant Strains / Electrochemical cultivation / Mercury-polluted soil treatment / Conner bioleaching |
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| Research Abstract |
Iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the most important bacteria for the bioleaching of copper and gold ores. In order to use the mercury reducing activity of A. ferrooxidans for the bioremediation of mercury, mercury-resistant A. ferrooxidans strains SUG 2-2 and MON-1 were screened among 150 strains of iron-oxidizing bacteria isolated from natural environments. It was found that strains SUG 2-2 and MON-1 have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity. Strain MON-I has volatilization activity an organomercurial lyase-like activity by cytochrome c oxidase. Nearly 100% of the total mercury in mercury-polluted soil or mercury wastewater was volatilized and recovered by incubating SUG 2-2 or MON-1 cells. suggesting that these mercury-resistant strains can be used for the bioremediation of inorganic and organic mercurial compounds.
A. ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide are dump in Chile. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm hat strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase. An electric cultivation device was developed. This was useful for the enzymatic refinement and the Applied technology.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Increase in Fe2+-Producing Activity during Growth of Acidithiobacillus ferrooxidans ATCC 23270 on Sulfur2008
Author(s)
Tsuyoshi, SUGIO, Taher, M., TAHA, Tadayoshi, KANAO, Fumiaki, TAKEUCHI
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Journal Title
Bioscience, Biotechnology, and Biochemistry 71
Pages: 2663-2669
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Existence of aa3-Type Ubiquinol Oxidase as a Terminal Oxidase in Sulfite Oxidation of Acidithiobacillus thiooxidans2006
Author(s)
Tsuyoshi, SUGIO, Tomohiro, HISAZUMI, Tadayoshi, KANAO, Kazuo, KAMIMURA, Fumiaki, TAKEUCHI, Atsunori, NEGISHI
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Journal Title
Bioscience, Biotechnology, and Biochemistry 70
Pages: 1584-1591
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Existence of aa_3-Type Ubiquinol Oxidase as a Terminal Oxidase in Sulfite Oxidation of Acidithiobacillus thiooxidans, Bioscience, Biotechnology, and Biochemistry2006
Author(s)
Tsuyoshi SUGIO, Tomohiro HISAZUMI, Tadayoshi KANAO, Kazuo KAMIMURA, Fumiaki TAKEUCHI, Atsunori NEGISHI
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Journal Title
Bioscience, Biotechnology, and Biochemistry 70,5
Pages: 1584-1591
Related Report
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[Journal Article] Isolation and same properties of sulfer dioxygenase from Acidithiobacillus thiooxidans NB1-32005
Author(s)
Tsuyoshi SUGIO, Kohta Ochi, Tadashi Muraoka, Fumiaki TAKEUCHI, Tadayoshi KANAO, Kazuo KAMIMURA and Atsunori
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Journal Title
In Proceedings of the 16th International Biohydrometallurgy Symposium. (Harrion, S. T. L, Rawlings, D. E., and J. Petersen, J. eds.) 70
Pages: 805-810
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Existence of ar Iron-oxidizing Bacterium Acidithiobacillus ferro-oxidans Resistant to Organomercurial Compounds2005
Author(s)
Fumiaki, TAKEUCHI, Atsunori, NEGISHI, Sosaku, NAKAMURA, Tadayoshi, KANAO, Kazuo, KAMIMURA, Tsuyoshi, SUGIO
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Journal Title
Journal of Bioscience and Bioengineering 99
Pages: 586-591
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Growth Inhibition by Tungsten in the Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans2005
Author(s)
Atsunori, NEGISHI, Tadashi, MURAOKA, Terunobu, MAEDA, Fumiaki, TAKEUCHI, Tadayoshi, KANAO, Kazuo, KAMIMURA, Tsuyoshi, SUGIO
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Journal Title
Bioscience, Biotechnology, and Biochemistry 69
Pages: 2073-2080
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Isolation and same properties of sulfer dioxygenase from Acidithiobacillus thiooxidans NB1-32005
Author(s)
Tsuyoshi, SUGIO, Kohta, Ochi, Tadashi, Muraoka, Fumiaki, TAKEUCHI, Tadayoshi, KANAO, Kazuo, KAMIMURA, Atsunori, NEGISHI
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Journal Title
In Proceedings of the 16th International Biohydrometallurgy Symposium. (Harrion, S. T. L, Rawlings, D. E., and J. Petersen, J. eds.) 70
Pages: 805-810
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] 児島湖浚渫土壌の酸性化を防止する試薬の開発2005
Author(s)
大内及亮.伊藤統博, 金尾忠芳, 上村一雄, 竹内文章, 杉尾 剛
Organizer
日本生物工学会大会
Place of Presentation
筑波市,つくば国際会議場
Year and Date
2005-11-16
Description
「研究成果報告書概要(和文)」より
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