pplication of Single-Molecule Observation for Kinetic Analysis of Genomic DNA Double-Strand Breaks in Relation to Genetic Activity

• Project/Area Number: 17510102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: International Pacific University (2007-2008); College of Nagoya Bunri University (2005-2006)
• Principal Investigator: YOSHIKAWA Yuko International Pacific University, 次世代教育学部, 教授 (80291871)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: ナノバイオ / 蛍光顕微鏡 / DNA二本鎖切断 / 単分子観察 / 活性酸素 / 長鎖DNA / 遺伝子機能 / 放射線損傷 / 抗酸化物質 / DNA高次構造 / 再構成クロマチン / 二本鎖切断 / ビタミンC / 原子間力顕微鏡

Research Abstract

蛍光顕微鏡を用いた単一分子観察法を活用することにより、ゲノムDNAのような巨大DNA分子(50kbp~200kbp程度)の二本鎖切断反応を、単分子観測法より定量的に観測・解析することの可能であることを明らかにした。これまでは、数kbp程度までのDNAでの二本鎖切断の計測がおこなわれるにとどまっていたが、本研究では100kbpに一箇所切断が入るような低損傷確率の条件下でも、信頼性のある速度論的な計測が可能となった。

Research Products

• [Journal Article] DNA Compaction Plays a Key Role in Radioprotection against Double-Strand Breaks as Revealed by Single-Molecule Observation (2008)
  • Author(s): Y. Yoshikawa, T. Mori, N. Magome, K. Hibino, K. Yoshikawa
  • Journal Title: Chem. Phys. Lett. 456 Pages: 80-83
  • NAID: 130006999678
  • Peer Reviewed
• [Journal Article] DNA Compaction Plays a Key Role in Radioprotection against Double-Strand Breaks as Revealed by Single-Molecule Observation (2008)
  • Author(s): Y. Yoshikawa
  • Journal Title: Chem. Phys. Lett. 456 Pages: 80-83
  • NAID: 130006999678
  • Peer Reviewed
• [Journal Article] DNA二重鎖切断反応の可視化 : ビタミンCによる抑制効果の定量的計測 (2007)
  • Author(s): 吉川祐子
  • Journal Title: ビタミン 81 Pages: 297-302
  • NAID: 110006368520
  • Peer Reviewed
• [Journal Article] 自己組織化するDNA (2007)
  • Author(s): 吉川祐子、吉川研一
  • Journal Title: 現代化学 2月号 No.431 Pages: 14-19
  • NAID: 40015255682
• [Journal Article] グルメの世界"を科学する (2007)
  • Author(s): 吉川研一、吉川祐子
  • Journal Title: 化学工学 71巻31-33 Pages: 0-0
  • NAID: 10018666337
• [Journal Article] Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation : Protective Effect of Antioxidative Compounds (2007)
  • Author(s): Y. Yoshikawa, E. Hirose, E. Sakai, C. Ikawa
  • Journal Title: Proceeding of 2007 International Symposium on Micro-Nanomehatronics and Human Science (MHS2007) Pages: 276-280
  • Peer Reviewed
• [Journal Article] Photo-induced DNA double-strand breaks monitored by single-molecule observation:protective effect of antioxidative compounds (2007)
  • Author(s): Yuko Yoshikawa
  • Journal Title: Proc.of the 2007 Int'1 Symp.on Micro-Nano Mechatronics and Human Science Pages: 276-280
• [Journal Article] DNA二重鎖切断反応の可視化:ビタミンCによる抑制効果の定量的計測 (2007)
  • Author(s): 吉川 祐子
  • Journal Title: ビタミン 81 Pages: 297-302
  • NAID: 110006368520
  • Peer Reviewed
• [Journal Article] 自己組織化するDNA (2007)
  • Author(s): 吉川祐子
  • Journal Title: 現代化学 431 Pages: 14-19
  • NAID: 40015255682
• [Journal Article] Protective Effect of Vitamin C against Double-Strand Breaks in Reconstituted Chromatin Visualized by Single-Molecule Observation (2006)
  • Author(s): Y. Yoshikawa, K. Hizume, Y. Oda, K. Takeyasu, S. Araki, K. Yoshikawa
  • Journal Title: Biophys. J. 90 Pages: 993-999
  • NAID: 120000896373
  • Peer Reviewed
• [Journal Article] Quantitative analysis of double-strand break in giant DNA by single-molecule observation (2006)
  • Author(s): Y. Yoshikawa, T. Mori, K. Yoshikawa
  • Journal Title: Proc. XIII Congress of the Society for Free Radical Research International ed. In Medimond International Proceedings Pages: 341-344
• [Journal Article] Na+ More Strongly Inhibits DNA Compaction by Spermidine (3+) than K^+ (2006)
  • Author(s): K. Hibino, Y. Yoshikawa, S. Murata, T. Saito, A. A. Zinchenko, K. Yoshikawa
  • Journal Title: Chem. Phys. Lett. 426 Pages: 405-409
  • NAID: 120000896364
  • Peer Reviewed
• [Journal Article] Na^+ more strongly inhibits DNA compaction by spermidine (3+) than K^+ (2006)
  • Author(s): K.Hibino
  • Journal Title: Chem. Phys. Lett. 426 Pages: 405-409
• [Journal Article] Quantitative analysis of double-strand break in giant DNA by single-molecule observation (2006)
  • Author(s): Y.Yoshikawa
  • Journal Title: Proc. XIII Congress of the Society for Free Radical Research International-SFRR 2006 Pages: 341-344
• [Journal Article] Protective Effect of Vitamin C against Double-Strand Breaks in Reconstituted Chromatin Visualized by Single-Molecule Observation (2006)
  • Author(s): Y.Yoshikawa
  • Journal Title: Biophys.J. 90 Pages: 993-999
  • NAID: 120000896373
• [Journal Article] Morphological Variation in a Toroid Generated from a Single Polymer Chain (2005)
  • Author(s): Y. Takenaka, K. Yoshikawa, Y. Yoshikawa, Y. Koyama, T. Kanbe
  • Journal Title: J. Chem. Phys. 123
  • Peer Reviewed
• [Journal Article] Morphological Variation in a Toroid Generated from a Single Polymer Chain (2005)
  • Author(s): Y.Takanaka
  • Journal Title: J.Chem.Phys. 123
• [Journal Article] Large Conformational Change on Giant DNA Induced by Ascorbic Acid : A Nobel Scheme on Its Antioxidative Activity (2005)
  • Author(s): Y.Yoshikawa
  • Journal Title: J.Biotech. 118
• [Journal Article] On/Off Switching of Transcription Caused by Folding Transition in Giant DNA : Novel Scenario on Genetic Control (2005)
  • Author(s): K.Yoshikawa
  • Journal Title: J.Biotech. 118
• [Presentation] 長鎖DNAの二重鎖切断を指標とした抗酸化能の定量的解析 : アスコルビン酸と他の抗酸化物質との比較検討へ向けて (2008)
  • Author(s): 吉川祐子
  • Organizer: ビタミンC研究委員会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-05
• [Presentation] 単分子観察によるDNAの放射線切断に関する研究 (2008)
  • Author(s): 森利明
  • Organizer: 大阪府立大学産学官連携機構・放射線研究センター平成19年度共同利用報告会
  • Place of Presentation: 堺
  • Year and Date: 2008-07-04
• [Presentation] 単分子観察によるDNAの放射線切断に関する研究 (2008)
  • Author(s): 森利明
  • Organizer: 大阪府立大学産学官連携機構・放射線研究センター平成19年度共同利用報告会
  • Place of Presentation: 大阪
  • Year and Date: 2008-07-04
• [Presentation] DNA compaction plays a key role in protection against double-strand break induced by ionizing radiation (2008)
  • Author(s): T. Mori
  • Organizer: 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: 福島
  • Year and Date: 2008-06-08
• [Presentation] DNA Compaction Plays a Key Role in Protection against Double-Strand Break Induced by Ionizing Radiation (2008)
  • Author(s): T. Mori
  • Organizer: 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: 福島
  • Year and Date: 2008-06-08
• [Presentation] DNA compaction plays a key role in protection against double-strand break as revealed by single-molecule observation (2008)
  • Author(s): Yuko Yoshikawa
  • Organizer: Gordon Research Conference: DNA Damage, Mutation&Cancer
  • Place of Presentation: Ventura,CA,USA
  • Year and Date: 2008-03-12
• [Presentation] DNA compaction plays a key role in protection against double-strand break as revealed by single-molecule observation (2008)
  • Author(s): Y. Yoshikawa
  • Organizer: Gordon Research Conference : DNA Damage, Mutation & Cancer
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2008-03-09
• [Presentation] 放射線による長鎖DNA二重鎖切断の新計測手法:糖付加水溶性ルチンおよびヘスペリジンによる抑制作用の定量的評価 (2007)
  • Author(s): 吉川 祐子
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 放射線による長鎖DNA二重鎖切断の新計測手法 : 糖付加水溶性ルチンおよびヘスペリジンによる抑制作用の定量的評価 (2007)
  • Author(s): 吉川祐子
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Radiation-induced double-strand break of giant DNA and its protection by antioxidative compounds (2007)
  • Author(s): Y. Yoshikawa
  • Organizer: 第1回アジア環境変異原学会・日本環境変異原学会第36回大会
  • Place of Presentation: 北九州
  • Year and Date: 2007-11-19
• [Presentation] Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation : Protective Effect of Antioxidative Compounds (2007)
  • Author(s): Y. Yoshikawa
  • Organizer: 18^<th> IEEE International Symposium on Micro-Nanomechatronics and Human Science (MHS2007)
  • Place of Presentation: 名古屋
  • Year and Date: 2007-11-12
• [Presentation] Photo-induced DNA double-strand breaks monitored by single-molecule observation:protective effect of antioxidative compounds (2007)
  • Author(s): Yuko Yoshikawa
  • Organizer: 18th IEEE International Symposium on Micro-Nano mechatronics and Human Science(MHS2007)
  • Place of Presentation: 名古屋
  • Year and Date: 2007-11-12
• [Presentation] Quantitative analysis of double-strand breaks in reconstituted chromatin by single-molecule observation (2007)
  • Author(s): Yuko Yoshikawa
  • Organizer: 16th International Chromosome Conference
  • Place of Presentation: Amsterdam,Netherland
  • Year and Date: 2007-08-27
• [Presentation] Quantitative analysis of double-strand breaks in reconstituted chromatin by single-molecule observation (2007)
  • Author(s): Y. Yoshikawa
  • Organizer: 16^<th> International Chromosome Conference
  • Place of Presentation: Amsterdam, the Netherlands
  • Year and Date: 2007-08-25
• [Presentation] 放射線照射による長鎖DNAの二重鎖切断と糖転移ルチンおよびヘスペリジンによる抑制作用 (2007)
  • Author(s): 吉川祐子
  • Organizer: 日本ビタミン学会第59回大会
  • Place of Presentation: 佐世保
  • Year and Date: 2007-05-25
• [Presentation] 単一分子観察法を用いた長鎖DNAの二重鎖切断反応の速度論的解析 (2007)
  • Author(s): 吉川祐子
  • Organizer: 核酸を中心としたケミカルバイオロジー
  • Place of Presentation: 京都
  • Year and Date: 2007-03-17
• [Presentation] 放射線によるDNAの二重鎖切断におよぼすDNAの高次構造 (2006)
  • Author(s): 森利明
  • Organizer: 第34回環境変異原学会
  • Place of Presentation: 堺
  • Year and Date: 2006-11-20
• [Presentation] DNA高次構造の自己組織化と生物活性 (2006)
  • Author(s): 吉川祐子
  • Organizer: 生理学研究所研究会「DNA構造を基盤とするゲノム生理学の展開-DNA,蛋白質、膜の相互作用」
  • Place of Presentation: 岡崎
  • Year and Date: 2006-11-09
• [Presentation] ビタミンCによるDNA二重鎖切断の抑制 : 単一分子観察法による定量的解析 (2006)
  • Author(s): 吉川祐子
  • Organizer: ビタミンC研究委員会
  • Place of Presentation: 和歌山
  • Year and Date: 2006-10-07
• [Presentation] 電離放射線によるDNA二重鎖切断と高次構造の関係について (2006)
  • Author(s): 森利明
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] Protective activity of vitamin C against double-strand breaks in reconstituted chromatin revealed by single-molecule observation (2006)
  • Author(s): Y. Yoshikawa
  • Organizer: 13th Biennial Congress of the International Society for Free Radical Research
  • Place of Presentation: Davos, Switzerland
  • Year and Date: 2006-08-15
• [Presentation] Na^+ shows higher effect than K^+ on inhibition of DNA compaction by spermidine(3^+) (2006)
  • Author(s): K. Hibino
  • Organizer: 20^<th> International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 京都
  • Year and Date: 2006-06-18
• [Presentation] 再構成クロマチンを用いた二本鎖切断反応の可視化とアスコルビン酸による抑制作用 (2006)
  • Author(s): 吉川祐子
  • Organizer: 日本ビタミン学会第58回大会
  • Place of Presentation: 徳島
  • Year and Date: 2006-05-27
• [Presentation] Relationship between Higher-order Structure of DNA Molecules and DNA Damage by Ionizing Radiation (2006)
  • Author(s): T. Mori
  • Organizer: The IXth International Workshop on Radiation Damage to DNA
  • Place of Presentation: Tekirova, Antalya, Turkey
  • Year and Date: 2006-05-13
• [Presentation] Direct visualization of double-strand breaks in reconstituted chromatin and the protective effect of ascorbic acid (2006)
  • Author(s): Y. Yoshikawa
  • Organizer: Workshop on Driven States in Soft and Biological Matter
  • Place of Presentation: Trieste, Italy
  • Year and Date: 2006-04-24
• [Presentation] Observation and Manipulation of Individual DNA Molecules : Unraveling the Intrinsic Property of Giant DNA (2005)
  • Author(s): Y. Yoshikawa
  • Organizer: Bio-Soft Matter Days 2005
  • Place of Presentation: 台北、台湾
  • Year and Date: 2005-12-19
• [Presentation] 再構成クロマチンにおける二本鎖切断反応の可視化とアスコルビン酸による抑制作用の定量的解析 : 単一分子鎖観察法の活用 (2005)
  • Author(s): 吉川祐子
  • Organizer: 第28回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2005-12-07
• [Presentation] DNA依存性プロテインキナーゼが介在する可逆的なDNA高次構造変換 (2005)
  • Author(s): 高萩真彦
  • Organizer: 日本生物物理学会第43回年会
  • Year and Date: 2005-11-23
• [Presentation] 単一分子鎖観察法による再構成クロマチンでの二本鎖切断反応の定量的解析 : アスコルビン酸による抑制作用 (2005)
  • Author(s): 吉川祐子
  • Organizer: 第34回日本環境変異原学会
  • Place of Presentation: 東京
  • Year and Date: 2005-11-16
• [Presentation] DNAの高次構造と放射線照射による損傷 (2005)
  • Author(s): 馬籠信之
  • Organizer: 第48回日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2005-11-15
• [Presentation] ビタミンCによって引き起こされるゲノムDNAの高次構造変化と遺伝子活性 (2005)
  • Author(s): 酒井博臣
  • Organizer: 第78回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-19
• [Presentation] 単一分子観察法を用いた長鎖DNA二本鎖切断反応の可視化と抗酸化ビタミンによる抑制作用 (2005)
  • Author(s): 鬼頭智子
  • Organizer: 第78回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2005-10-19
• [Presentation] Large Conformational Change on Giant DNA Induced by Ascorbic Acid : A Nobel Scheme on Its Antioxidative Activity (2005)
  • Author(s): Y. Yoshikawa
  • Organizer: The 12^<th> European Congress on Biotechnology
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2005-08-21
• [Presentation] DNAの高次構造変化と二本鎖切断反応-1分子可視化法の活用 (2005)
  • Author(s): 吉川祐子
  • Organizer: 放射線施設共同利用報告会
  • Place of Presentation: 堺
  • Year and Date: 2005-06-27
• [Presentation] アスコルビン酸によって引き起こされるゲノムDNAの高次構造変化と抗酸化作用 (2005)
  • Author(s): 吉川祐子
  • Organizer: 日本ビタミン学会第57回大会
  • Place of Presentation: 志摩
  • Year and Date: 2005-05-26
• [Presentation] DNAの高次構造制御 : 非ウィルスベクターの設計への応用 (2005)
  • Author(s): 吉川祐子
  • Organizer: 遺伝子・デリバリー研究会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2005-05-21
• [Presentation] Marked Structural Chamge on Giant DNA Induced by Ascorbic Acid : Nobel Scheme on Its Antioxidant Activity (2005)
  • Author(s): Y. Yoshikawa
  • Organizer: Human Genome Meeting 2005
  • Place of Presentation: 京都
  • Year and Date: 2005-04-18
• [Book] 『栄養・食糧学用語辞典』日本栄養・食糧学会編(項目分担 : 総著者数283名) (2007)
  • Author(s): 吉川祐子
  • Total Pages: 808
  • Publisher: 建帛社
• [Book] 栄養・食糧学用語辞典 (2007)
  • Author(s): 日本栄養・食糧学会編
  • Total Pages: 808
  • Publisher: 建帛社
• [Book] "Physical Chemistry of DNA-Carrier Complex" in Non-viral Gene Therapy : Gene Design and Delivery(Eds. K. Taira, K. Kataoka, T. Niidome) (2005)
  • Author(s): K. Yoshikawa, Y. Yoshikawa
  • Publisher: Springer-Verlag, Tokyo
• [Book] Non-viral Gene Therapy : Gene Design and Delivery (2005)
  • Author(s): K.Taira (Ed.)
  • Total Pages: 487
  • Publisher: Springer-Verlag, Tokyo