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Structural and functional elucidation of novel immunobiologically active high molecular weight glycoconjugatos from bacterial cell surface

Research Project

Project/Area Number 17510179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKagoshima University

Principal Investigator

SUDA Yasuo  Graduate School of Science and Technology, Kagoshima University, Professor, 理工学研究科, 教授 (70179282)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Masahito  Kagoshima University, Graduate School of Science and Technology, Associate Professor, 大学院理工学研究科, 助教授 (30333537)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsGram-positive bacteria / innate immunity / lipoteichoic acid / lipoprotein / lipopeptide / lipoarabinomannan / separation and purification / neutralizing antibody
Research Abstract

Many glycoconjugates are located on cell surface of bacteria and shown to activate innate immune system. Lipoteichoic acid (LTA) is a predominant glycoconjugate in Gram-positive bacteria and reported to possessed immunobiological activities. We previously reported that a purified LTA obtained from Enterococcus hirae had no immunostimulatingactivity.butasubfractionintheLTAfraction exerted the activity. In this study, we intended to elucidate a structure and function of the compound responsible for the activity.
We established an antibody neutralizing the activity of the active compound. The antibody neutralized not only LTA fraction from E. hirae but also that from.Staphylococcus aureus. The antibody also inhibited the activity of immunostimulating synthetic lipopeptides. By immunoblotting assay, it is found that the antibody recognized lipopeptides but not their non-lipidated counterparts. These results suggest that lipoprotein-like compounds are responsible for the activity of the LTA fraction.
Lipoi3rabinomannan (LAM) is a major high molecular weight glycoconjugates in acid-fast bacilli and is also reported to cause immune activation. The principal structure for the activity, however, has not been described. In this study, we aimed to elucidate the structure.
The LAM was extracted from Mycobacterium smegmatis and subjected to further purification. A highly active compounds were found in a high-anionic fraction and the activity was abrogated by protease digestion. These results indicated that the proteinous materials are active components in LAM fraction.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2007 2006

All Journal Article (5 results)

  • [Journal Article] Evidence of immunostimulating lipoprotein co-existing in natural lipoteichoic acid fraction.2007

    • Author(s)
      Masahito Hashimoto et al.
    • Journal Title

      Infection and Immunity 75(4)

      Pages: 1926-1932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Evidence of immunostimulating lipoprotein co-existing in natural lipoteichoic acid fraction.2007

    • Author(s)
      Masahito Hashimoto et al.
    • Journal Title

      Infection and Immunity 75-4

      Pages: 1926-1932

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lipoprotein is a predominant Toll-like receptor 2 ligand in Staphylococcus aureus cell wall components.2006

    • Author(s)
      Masahito Hashimoto et al.
    • Journal Title

      International Immunology 18

      Pages: 355-362

    • NAID

      10020621607

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Not lipoteichoic acid but lipoproteins appear to be the dominant immunobiologically active compounds in Staphylococcus aureus.2006

    • Author(s)
      Masahito Hashimoto et al.
    • Journal Title

      The Journal of Immunology 177

      Pages: 3162-3169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Not lipotcichoic acid but lipoproteins appear to be the dominant immunobiologically active compounds in Staphylococcus aureus.2006

    • Author(s)
      Masahito Hashimoto et al.
    • Journal Title

      The Journal of Immunology 177

      Pages: 3162-3169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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