| Project/Area Number |
17510191
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka Medical Center for Cancer and Cardiovascular Diseases Osaka Prefectural Hospital Organization |
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Principal Investigator |
MIYAMOTO Yasuhide Osaka Medical Center for Cancer and Cardiovascular Diseases Osaka Prefectural Hospital Organization, Research Institute, chief researcher, 研究所, 総括研究員 (90322742)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | colon cancer / sugar chain / mass spectrometry / glycosphingolipid / laser microdissection / 癌 |
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| Research Abstract |
We have established microanalyses methods to analyze structures of N-glycans and acidic glycosphingolipids(GSLs). Acidic GSLs from three cases of primary colonic adenocarcinoma and hepatic metastasis were analyzed. A total of 22 major acidic GSL structures were identified, and their relative quantities were revealed in detail. They are composed of one sulfated (SM3), one lacto-series (SLe^a), six kinds of ganglio-series and fourteen kinds of neolacto-series GSLs. They include most of the acidic glycosphingolipids previously reported to be present in the tissues and two hitherto unknown fucogangliosides sharing the same terminal structure : NeuAcα2-6(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4Glc, and NeuAcα2-6(Fucα1-2)Galβ1-4GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3-Galβ1-4Glc. Laser microdissection technique enabled to revealed that α2-6 terminal sialylated and fucosylated GSLs were overexpressed in colon cancer cells.
Slight difference of N-glycan structures between normal colon epithelia and tumor tissues was observed. However, comparison of the various N-glycans profiles with the aid of LMD identified seven characteristic N-glycans with significantly different expression profiles between normal and cancerous cells that could not be detected by conventional analysis. These N-glycans were bi-antennary having bisecting GlcNAc and decreased in colon cancer cells.
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