| Project/Area Number |
17540385
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics/Chemical physics
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| Research Institution | NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST) |
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Principal Investigator |
NADA HIROKI NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), RESEARCH INSTITUTE FOR ENVIRONMENTAL MANAGEMENT AND TECHNOLOGY, SENIOR RESEARCHER, 環境管理技術開発部門, 主任研究員 (90357682)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA YOSHINOR HOKKAIDO UNIVERSITY, INTSITUTE OF LOW TEMPERATURE SCIENCE, PROFESSOR, 低温科学研究所, 教授 (20113623)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ANTIFREEZE PROTEIN / ICE CRYSTAL / CRYSTAL GROWTH / MOLECULAR DYNAMICS / 不凍糖タンパク質 |
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| Research Abstract |
THE PURPOSE OF THIS PROJECT WAS TO ELUCIDATE THE MECHANISM OF INHIBITION OF ICE GROWTH BY ANTIFREEZE GLYCOPROTEINS (AFGP) AND ANTIFREEZE PROTEINS (AFP) AT THE MOLECULAR LEVEL. WE CARRIED OUT MOLECULAR DYNAMICS (MD) SIMULATIONS OF ICE-WATER INTERFACES IN THE PRESENCE OF AFPS. THE SIMULATIONS WERE CARRIED OUT FOR THE INTERFACES OF PYRAMIDAL {2021} AND PRISMATIC (1010) PLANES OF ICE.
SIMULATION RESULTS CLEARLY INDICATED THAT, DURING THE GROWTH OF ICE ON THE INTERFACE, THE AFP WAS PARTLY VARIED INTO THE GROWING ICE. THE INHIBITION OF ICE GROWTH OCCURRED ONLY ON THE PYRAMIDAL PLANE, WHEN THE AFP WAS ALIGNED ALONG THE <0112> VECTOR. THE INHIBITION OF ICE GROWTH WAS DUE TO THE GIBBS-THOMSON EFFECT, I.E., THE MELTING POINT DEPRESSION AT CURVED INTERFACES OF ICE, WHICH WAS CAUSED BY THE STABLE LOCALIZATION OF THE AFP. THE STABLE LOCALIZATION OF THE AFP ORIGINATED FROM THE THREE-DIMENSIONAL FIT OF THE AFP TO THE SURROUNDING ICE.
IN ADDITION, WE ALSO CARRIED OUT AN EXPERIMENT STUDY OF ICE GROWTH IN THE PRESENCE OF AFGPS. THE RESULTS INDICATED THAT AFGPS INHIBIT THE GROWTH OF PYRAMIDAL PLANES OF ICE. THE AFPS ALSO INHIBIT THE GROWTH OF PYRAMIDAL PLANES OF ICE. THEREFORE, WE CONCLUDE THAT THE MECHANISM OF INHIBITION OF ICE GROWTH BY THE AFGPS IS ESSENTIALLY THE SAME AS THE MECHANISM FOR THE AFPS, WHICH WAS ELUCIDATED BY THE PRESENT MD SIMULATIONS.
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