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The environmental pollution material which assumed NO produced by a cultured cell an index and a food composition

Research Project

Project/Area Number 17550146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental chemistry
Research InstitutionYamagata Promotional Organization for Industrial Technology

Principal Investigator

YOSHITAKE Jun (2007)  Yamagata Promotional Organization for Industrial Technology, Research and Development Sectionl, Researcher (70414349)

吉村 哲彦 (2005-2006)  (財)山形県産業技術振興機構, 研究開発部, 部長 (70271517)

Co-Investigator(Kenkyū-buntansha) SHIGENOBU Kasai  東北工業大学, Tohoku Institute of Technology, Environmental information engineering department, Associate professor (70342730)
TOMOKAZU Matsuei  Tokoku University, Environmental information engineering department, Professor (70173797)
吉武 淳  (財)山形県産業技術振興機構, 研究開発部, 研究員 (70414349)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsnitrogenmonoxide (NO) / endocrine disrupting chemicals (EDCs) / macrophages / lipopolysaccharide / estrogen receptor-dependent repressor / 8-ニトログアノシン / シグナル伝達系
Research Abstract

Since the endocrine and immune systems share portions of some intracellular signal pathways, endocrine disrupting chemicals (EDCs) are considered potential agents for influencing inflammatory responses. Here, we investigated the effect of EDCs on lipopolysaccharide (LPS)-induced NO production and NF-κB activation in the RAW264.7 mouse macrophage cell line. Five phenol-containing EDCs were investigated, namely bisphenol A (BPA), the alkyl phenols p-n-nonylphenol (NP) and p-n-octylphenol (OP), and the chlorinated phenols 2,4-dichrolophenol (DCP) and pentachlorophenol (PCP). Our results revealed that these chemicals dose-dependently suppressed LPS-induced NO production, as reflected by decreased NOx content. The suppressive effects of BPA, NP and OP, but not PCP or DCP, were blocked by the estrogen receptor (ER) inhibitor, ICI182780. ELISA-based quantification of the DNA-binding activity of free p65 NF-κB showed that LPS-induced NF-κB activation was significantly diminished by EDC treatment. Furthermore, immunocytochemical analysis of 8-nitroguanosine, a unique index of NO-mediated signaling, showed that 8-nitroguanosine formation increased in LPS-stimulated cells, but this increase was inhibited by the tested EDCs. These results demonstrate that EDCs suppress NO production and NF-κB activation in LPS-stimulated macrophages through ER-dependent (BPA, NP, OP) and -independent (PCP, DCP) pathways. The EDCs further inhibited 8-nitroguanosine formation, suggesting that they interfere with NO-mediated signaling. Thus, EDCs might play important roles in the inflammatory response and host defense system against foreign pathogens.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 2005

All Journal Article (14 results) (of which Peer Reviewed: 6 results) Presentation (4 results) Book (2 results)

  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages : involvement of estrogen receptor-dependent or -independent pathways.2008

    • Author(s)
      J.Yoshitake
    • Journal Title

      Nitric Oxide 18

      Pages: 223-228

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrine-disrupting chemicals in LPS-stimulated macrophages:involvement of estrogen receptor-dependent or-independent pathways2008

    • Author(s)
      J. Yoshitake
    • Journal Title

      Nitric Oxide (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toll-like receptor(TLR)2 induced through TLR4 signaling initiated by Helico-bacter pylori cooperatively amplifies iNOS induction in gastric epithelial cells.2007

    • Author(s)
      K.Uno
    • Journal Title

      Am.J.Physiol.Gastrointest. Liver Physiol. 293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Disruption of gastric barrier function by luminal nitrosative stress : A potential chemical insult to human gastro-oesophageal junction.2007

    • Author(s)
      N.Ara
    • Journal Title

      Gut 57

      Pages: 306-313

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Toll-like receptor(TLR)2 induced through TLR4 signaling initiated by Helico-bacter pylori cooperatively amplifies iNOS induction hl gastric epithelial cells2007

    • Author(s)
      K. Uno
    • Journal Title

      Am. J. Physiol. Gastrointest. Liver Physiol. 293

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disruption of gastric barrier function by luminal nitrosative stress:A potential chemical insult to human gastro-oesophageal junction2007

    • Author(s)
      N. Ara
    • Journal Title

      Gut 57

      Pages: 306-313

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fe-S cluster proteins are intracellular targets for nitric oxide generated luminally at the gastro-oesophageal junction2007

    • Author(s)
      K.Asanuma
    • Journal Title

      Nitric Oxide In press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ^<17>O NMR Study of Oxo Metalloporphyrin complexes : Correlation with Electronic Structure of M=O Moiety2006

    • Author(s)
      H.Fujii
    • Journal Title

      J.Inorg.Biochem 100

      Pages: 533-541

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The Formation of g=2.49-species of Cytochrome P450 in the Rat Liver by PCB126 Oral Administration : Indentification of Heme Axial Ligands by EPR spectroscopy2006

    • Author(s)
      H.Morita
    • Journal Title

      Biosci.Biotech.Biochem 70

      Pages: 2974-2981

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sequential changes of nitric oxide levels in the temporal lobe on applying nitric oxide synthase inhibitors and phenobarbital in kainic acid-treated mice2005

    • Author(s)
      N.Kato
    • Journal Title

      Epilep.Res. 65

      Pages: 81-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1)2005

    • Author(s)
      S.Kasai
    • Journal Title

      Anal.Chim Acta 549

      Pages: 14-19

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Diffusion of cytotoxic concentrations of nitric oxide generated luminally at the gastro-esophageal junction of rats2005

    • Author(s)
      K.Asanuma
    • Journal Title

      Gut, 54

      Pages: 1072-1077

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a heme redox state-independent mechanism2005

    • Author(s)
      D.Suto
    • Journal Title

      Biochem.J. 392

      Pages: 399-406

    • Related Report
      2005 Annual Research Report
  • [Journal Article] からだの中のラジカルを電子スピン共鳴(ESR)法で見る-ラジカル画像化の方法と最近の進歩2005

    • Author(s)
      吉村哲彦
    • Journal Title

      化学と教育 53

      Pages: 562-565

    • Related Report
      2005 Annual Research Report
  • [Presentation] LPS誘導NO産生および8-ニトログアノシン形成におけるフェノール含有内分泌かく乱物質の影響2007

    • Author(s)
      吉武淳
    • Organizer
      第29回日本フリーラジカル学会学術集会/日本過酸化脂質・フリーラジカル学会第31回大会合同学会
    • Place of Presentation
      愛知県 名古屋市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] フェノール含有内分泌かく乱物質がLPS誘導NO産生経路に及ぼす影響2007

    • Author(s)
      吉武淳
    • Organizer
      第80回日本生化学会大会
    • Place of Presentation
      神奈川県 横浜市
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] LPS誘導NO産生および8-ニトログアノシン形成におけるフェノール含有内分泌かく乱物質の影響2007

    • Author(s)
      吉武 淳
    • Organizer
      第29回日本フリーラジカル学会学術集会/日本過酸化脂質・フリーラジカル学会第31回大会合同学会
    • Place of Presentation
      愛知県名古屋市
    • Related Report
      2007 Annual Research Report
  • [Presentation] フェノール含有内分泌かく乱物質がLPS誘導NO産生経路に及ぼす影響2007

    • Author(s)
      吉武 淳
    • Organizer
      第80回日本生化学会大会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2007 Annual Research Report
  • [Book] 生体内NOのレドックス種および活性酸素窒素種の反応と活性-最近のシンポ、別冊・医学のあゆみ「酸化ストレスーフリーラジカル医学生物学の最前線Ver.2」2006

    • Author(s)
      吉村哲彦
    • Total Pages
      4
    • Publisher
      医歯薬出版
    • Related Report
      2006 Annual Research Report
  • [Book] 電子スピン共鳴(ESR,EPR)「生命元素の事典」(桜井弘編)2006

    • Author(s)
      吉村哲彦
    • Total Pages
      6
    • Publisher
      オーム社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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