| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
1. For the immobilization of glycan chains on the core, reductive amination between the amino groups of chitosan-derived hexamer as the core substance and heparin as the ligand was investigated. Due to the toxicity of sodium cyanoborohydride usually utilized for such system, use of sodium triacetoxiborohydride as an alternative reducing reagent is considered.
2. Cross-linking of sulfated polysaccharide using poly(ethyleneglycol)diglycidylether was investigated. While agarose, a neutral polysaccharide, was cross-linked successfully with this reagent, cross-linking of the sulfated polysaccharides such as kappa-and lambda-carrageenans and funoran, the 6-sulfated agarose from the red seaweed Gloiopeltis complanata, was unsuccessful. Interference of such cross-linking would not be due to the configuration of the polysaccharide chain and ionic property of the sulfate group, but due to steric hindrance of the bulky sulfate groups.
3. Sulfated polysaccharide from the red seaweed Ceratodictyon sponguisum was isolated and characterized. This polysaccharide was shown to contain 3,6-anhydrogalactose residue, sulfate group linked to 0-4 of the galactose residue and other sulfate groups of equatrial configuration on the basis of the results from infrared spectrum.
4. Five acidic polysaccharide fractions was isolated from the mucilage of Morus alba leaves. The main fraction purified homogeneously appeared to the polysaccharide containing rhamnose, galactose, glucose, galacturonate and glucuronate. The covalent structure of this polysaccharide was partly elucidated. In addition, this polysaccharide appeared to be localized in particular epidermal cells of the leaves from the immunohistological observation, and was accumulated in 20% of total epidermal cells of the mature leaves.
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