Development of advanced Scaffolds for Bone Tissue Regeneration Using Biodegradable Composites and Its Functional Evaluation.
| Project/Area Number |
17560070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Materials/Mechanics of materials
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| Research Institution | Kobe University |
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Principal Investigator |
HIWA Chiaki Kobe University, Organization of advanced Science and Technology, Assistant Professor (80294198)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAI Yoshikazu Kobe University, Graduate School of Engineering, Professor (90155656)
ADACHI Taiji Kyoto University, Graduate School of Engineering, Associate Professor (40243323)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Biodegradable Materials / Tissue Engineering / Scaffold / Composite Materials / 歯槽骨の再生 / 生分解樹脂 / 骨組織生成 |
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| Research Abstract |
We developed novel fabric structural composite scaffolds using a bioabsorbable polymer for bone tissue regeneration. This scaffold consists of a combination of high strength fiber fabric and resin coat. Bioabsorbable resins used in this investigation are Poly Lactic-Acid (PLA), ε-polycaprolactone (PCL) and Poly lactic-polyglycolic-acid copolymer (PLGA). The rigidity and yield strength of scaffold are increased owing to the bonding of fiber intersection by resin coat. In the aim of evaluation of cell proliferation and differentiation on scaffolds, mouse osteoblast-like cells (MC3T3-E1) were seeded onto the scaffolds and cultured in vitro for six weeks. The cells proliferated during the time in culture and formed a space-filling tissue between polymer fibers. Bone regenerative messenger ALP/DNA levels remained high compared with those one of culture dish. Mineralization of the deposited collagen on scaffold was initially observed at four weeks and subsequently increased, and also rigidity and strength to the radial direction of scaffold increased except in case of PLGA composite scaffold. Culture of cell on scaffold led to formation of a bone tissue for six weeks except PLGA composite scaffold. This reason is sake of a fast strength decrease of PLGA resin by hydrolysis and consequently the cell loses a scaffold to be deposited of calcium.
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Application of Novel Fabric Structural Composites for Regenerative Medical Scaffold, -Development of Tubular Knitted Fabric Composites Made of Biodegradable Polymers2008
Author(s)
Nobuo, Tanaka, Chiaki, Hiwa, Toshiyuki, Imoto, Taiji, Adachi, Masaki, Hojo, Shunsuke, Baba
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Journal Title
Material Systems Vol. 26
Pages: 79-88
Description
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[Journal Article] Application of Novel Fabric Structural Composites for Regenerative Medical Scaffold, -Formation Process of Bone Tissue by Mouse Osteoblast-like Cell(MC3T3-E 1)2008
Author(s)
Chiaki, Hiwa, Terumasa, Okizoe, Yoshikazu, Nakai, Nobuo, Tanaka, Adachi, Masaki, Hojo, Shunsuke, Baba
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Journal Title
Material Systems Vol. 26
Pages: 89-95
Description
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[Journal Article] Framework for Optimal Design of Porous Scaffold Microstructure by Computational Simulation of Bone Regeneration2006
Author(s)
Taiji, Adachi, Yuki, Osako, Mototsugu, Tanaka, Masaki, Hojo, Scott, J. Hollister
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Journal Title
Biomaterials Vol. 27, No. 21
Pages: 3964-3972
Description
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[Presentation] Development of the Three-dimensional Woven Scaffold and The Evaluation of Its Functional Properties2008
Author(s)
Kumiko, Yokota, Chiaki, Hiwa, Nobuo, Tanaka, Yoshikazu, Nakai, Taiji, Adachi, Masaki, Hojo
Organizer
JCOM-37 Symposium of JSMS
Place of Presentation
Japan
Description
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[Presentation] Cell Growth of Mouse Osteoblastic Cells on in vitro of Tree Dimensional Fabric Structural Composite scaffolds2007
Author(s)
Chiaki, Hiwa, Kumiko, Yokota, Nobuo, Tanaka, Toshiyuki, Imoto, Taiji, Adachi, Masaki, Hojo
Organizer
The 56th Annual Meeting of the JSME
Place of Presentation
Japan
Description
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[Presentation] Biodegradable Properties of PLA Fiber Composites Using Different Binder Resin2007
Author(s)
Chiaki, Hiwa, Katsuhiko, Takenouchi, Yosikazu, Nakai, Tanaka, Taiji, Adachi, Masaki, Hojo
Organizer
M&M2007 conference of Material Mechanics of JSME
Place of Presentation
Japan
Description
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[Presentation] Cell Proliferation of Mouse Osteoblast-like Cells on in vitro of Tree Dimensional Fabric Structural Composite scaffolds2007
Author(s)
Chiaki, Hiwa, Kumiko, Yokota, Nobuo, Tanaka, Toshiyuki, Imoto, Taiji, Adachi, Masaki, Hojo
Organizer
The 29th Annual Meeting of the Japanese Society for Biomaterials
Place of Presentation
Japan
Description
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[Patent(Industrial Property Rights)] 三次元製織装置および三次元製織方法2006
Inventor(s)
日和千秋, 北條正樹, 安達泰治, 馬場俊輔, 井上剛臣, 井元俊之
Industrial Property Rights Holder
科学技術振興機構, 神戸大学, 京都大学, オステオジェネシス, 井元製作所, 新産業創造機構
Industrial Property Number
2006-214247
Filing Date
2006-08-07
Description
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[Patent(Industrial Property Rights)] 組織再生用複合化スカフォールド2005
Inventor(s)
日和千秋, 北條正樹, 安達泰治, 馬場俊輔, 井上剛臣, 井元俊之
Industrial Property Rights Holder
科学技術振興機構, 神戸大学, 京都大学, オステオジェネシス, 井元製作所, 新産業創造機構
Industrial Property Number
2005-378936
Filing Date
2005-12-28
Description
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