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Research on the driving, lubrication and self assembled mechanism of bacterial flagellar motor

Research Project

Project/Area Number 17560115
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Design engineering/Machine functional elements/Tribology
Research InstitutionTokyo Institute of Technology

Principal Investigator

NAKANO Takashi  Tokyo Institute of Technology, Graduate School of Information Science and Engineering, Research associate, 大学院情報理工学研究科, 助手 (00183517)

Co-Investigator(Kenkyū-buntansha) MOMOZONO Satoshi  Tokyo Institute of Technology, Graduate School of Science and Engineering, Research associate, 大学院理工学研究科, 助手 (70262300)
AIZAWA Shin-ich  Prefectural University of Hiroshima, Faculty of Life and Environmental Sciences, Professor, 環境生命学部, 教授 (50222451)
YOSHIMURA Hideyasu  Meiji University, Faculty of Science and Engineering, Professor, 理工学部, 教授 (70281441)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsFlagellar Motor / Diffusive Double Layer / Nano Machine / Non classical lubrication / Self Assembled System / PL ring / Universal Joing / Tribology / バイオナノマシン / トライボロジー
Research Abstract

In this research, we investigated the driving, lubrication and self assembled mechanism of bacterial flagellar motor in order to develop new principle of nanomachine architecture. In nanoscale world, different kind of physical effects have essential roles on driving, lubrication and self assembled mechanism of machines. We did not know the guiding principle to construct nonomachine at the starting point of the study. Bacterial having flagellar motor sometimes called living nanomachine as it has motor, journal bearing universal joint and screw. Our main idea is to get the precise knowledge of the mechanism of bacterial flagellar motor and utilizing these mechanisms to the driving, lubrication and self assembled system of nanomachines.
In a previous study, we estimated lubrication mechanism of bacterial flagellar. By using theory of the classical hydrodynamic lubrication, we calculated load capacitance. Resulting load capacitance is order of 0.1pN( x 10^<-12>N) and this values is not adequate to compensate driving force (0.5pN) Furthermore, intermolecular force between rod and journal becomes order of 10^2pN that is far bigger than load generated by driving force.
Thus, we concluded that new lubrication mechanism must be introduced to explain the behavior of PL ring. We proposed use of diffusive double layer as possible candidate of lubrication mechanism. We estimated the load capacitance generated by this effect and showed the adequate amount of repulsive force can be obtained by this mechanism.
We formulated new lubrication mechanism using the effects of diffusive double layer that is known as the origin of stability in hydrophobic colloidal solution. It is shown that this mechanism not only gives enough load capacitance to compensate the various forces between journal of rod in axial and radial direction but also gives driving force of self assembled system.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2006 2005 Other

All Journal Article (5 results)

  • [Journal Article] バクテリアベん毛モータの運動を通して量子は見えるか2006

    • Author(s)
      中野 隆, 桃園 聡, 吉村 英恭, 相沢 慎一
    • Journal Title

      トライボロジー会議予稿集 2006-5

      Pages: 169-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] バクテリアべん毛モータの潤滑駆動機構を通して量子は見えるか2006

    • Author(s)
      中野隆, 桃園聡, 吉村英恭, 相沢慎一
    • Journal Title

      トライボロジー会議予稿集 東京 2006-5

      Pages: 169-170

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 酸化チタン表面での水分子の分解機構の理論的研究II2005

    • Author(s)
      中野 隆, 森 和秀 他
    • Journal Title

      本コンピュータ化学会2005年秋季大会予稿集

      Pages: 90-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of Diffusive Double Layer on the Nano and Bio-nao Machines2005

    • Author(s)
      Takashi NAKANO, Satoshi MOMOZONO, Shin-ich AIZAWA, Hideyasu YOSHIMURA
    • Journal Title

      International Tribology Conference KOBE 2005 Book of Synopses

      Pages: 268-268

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Can we observe the quantum mechanical effects through the and Hideyasu Yoshimura motion of bacterial flagellar motor

    • Author(s)
      Takashi NAKANO, Satoshi MOMOZON, Shin-ich Aizawa, Hideyasu Yoshimura
    • Journal Title

      Proceedings of JAST Tribology Conference Tokyo 2006-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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