| Project/Area Number |
17560660
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Okayama University |
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Principal Investigator |
KITAMURA Yoshiro Okayama University, Department of Environmental Science & Technology, Professor, 大学院環境学研究科, 教授 (90032945)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIZAWA Hidekazu Okayama University, Department of Environmental Science & Technology, Professor, 大学院環境学研究科, 教授 (20244262)
NISHINO Satoru Nara National College of Technology, Department of Chemical Engineering, Assistant Professor, 物質科学工学科, 助手 (20413817)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Nanocapsule / Biodegradable polymer / Polylactide / Polymer surfactant / Colloid / Drug delivery system / Polymer microparticle / Surface chemistry |
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| Research Abstract |
This research was conducted to clarify the effect of process parameters which affected the release and dispersion properties of functional polylactide nanocapsules
At first, poly(ethyleneoxide monooleate-block-D, L-lactide) (MOPEO-PLA) was synthesized in the presence of stannous 2-ethylhexanoate catalyst. Amphiphilic MOPEO-PLA would have potential to control of various characteristics of polymer nanocapsules, surface and internal structure, surface morphology, release property and so on, by utilizing the surfactant property and the reactive double bond. Furthermore, the interfacial tension measurement of MOPEO-PLA-toluene/water system revealed that MOPEO-PLA had a good surface activity which was almost equal to that of MOPEO. The MOPEO-PLA/PLA blend films were prepared by solvent-cast on water layer. It was confirmed from contact angle measurement that the hydrophilic PEO segments were selectivity accumulated to the oil-water interface.
Secondly, protein encapsulation was tried and proteins were effectively extracted by using self-assembly of synthesized MOPEO-PLA in the oil phase. The water content in the chloroform phase dramatically increased with the MOPEO-PLA concentration, indicating that a MOPEO-PLA microemulsion was formed with a large amount of water molecules. The largest amount of cytochrome c in the MOPEO-PLA microemulsion phase was extracted when the pH of the aqueous phase was close to p/ of cytochrome c, and the extraction performance increased at higher temperatures. From these results, the main factor for the extraction of cytochrome c was found to be the hydrophobic interaction between MOPEO-PLA and cytochrome c. This MOPEO-PLA microemulsion provides a benign extraction process for proteins. A MOPEO-PLA microemulsion system would enable a new encapsulation system in a biodegradable polymer matrix for delivery of proteins and peptides.
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